[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(08):79-84.[doi:10.11942/j.issn1002-2767.2021.08.0079]
甘草愈伤组织的诱导与再分化研究
- 文献标志码:
- A
- 摘要:
- 为提高甘草育种效率，促进优良品种的保存和开发利用，以甘草（Glycurrihiza uralensis Fisch.）无菌苗的愈伤组织为材料，通过正交试验比较不同激素组合对甘草愈伤组织再分化的影响，探究愈伤组织再分化的最适条件。结果表明：生芽最佳培养基为MS+IBA 0.4 mg?L-1+IAA 0.4 mg?L-1+KT 0.3 mg?L-1，芽数4.87个?块-1，芽长2.32 cm，诱导率达64.44%；诱导甘草不定根最佳培养基配方为MS+IBA 0.4 mg?L-1 +IAA 0.4 mg?L-1 +KT 0.1 mg?L-1，根数5.67条?块-1，根长3.56 cm，诱导率达55.56%。
相似文献/References:
[1]王玉洋,蒋柳青,董雪梅,等. 不同因素对水稻愈伤组织形成的影响[J].黑龙江农业科学,2013,(01):11.
WANGYu-yang,JIANGLiu-qing,DONGXue-mei,et al.EffectofDifferentFactorsonRiceCallusFormation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(08):11.
[2]陈文武.火棘离体叶片愈伤组织的诱导研究[J].黑龙江农业科学,2014,(07):21.
CHEN Wen-wu.Study on Inducing Callus of Vitro Leaves of Pyracantha fortuneana[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):21.
[3]王琳,关云.辐射徐长卿愈伤组织的研究[J].黑龙江农业科学,2014,(04):14.
WANG Lin,GUAN Yun.Study on Cynanchum paniculatum Callus by Radiation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):14.
[4]魏锐,何耀奇,彭丹,等.不同激素配比对花生种胚的离体培养与植株再生的影响[J].黑龙江农业科学,2012,(09):21.
WEI Rui,HE Yao-qi,PENG Dan,et al.Effect of Different Plant Hormones Combinations on the Peanut Embryo in vitro Culture and Plant Regeneration[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(08):21.
[5]刘晓晨,曹君迈,陈彦云,等.罗布麻外植体的筛选及愈伤组织诱导[J].黑龙江农业科学,2012,(09):24.
LIU Xiao-chen,CAO Jun-mai,CHEN Yan-yun,et al.Explants Selection and Callus Induction of Apocynum venetum L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(08):24.
[6]龚娜,杨涛,肖军,等.非洲菊花托愈伤组织的诱导研究[J].黑龙江农业科学,2011,(03):21.
GONG Na,YANG Tao,XIAO Jun,et al.Study on Callus Induction of Gerbera Jiamesonii Receptacle[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(08):21.
[7]邱玲玉.天人菊无性系建立的研究[J].黑龙江农业科学,2010,(12):1.
QIU Ling-yu.Study of Establishment of Asexual Line for Gaillardia pulchella[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(08):1.
[8]谢礼洋,匡华琴,赖钟雄,等.辣椒愈伤组织诱导的研究[J].黑龙江农业科学,2013,(11):17.
XIE Li-yang,KUANG Hua-qin,LAI Zhong-xiong,et al.Study on Callus Induction from in vitro Capsicum annuum L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(08):17.
[9]宋艳东,刘青,赵恒寿.鸡血藤和甘草制剂对金鱼及泥鳅急性毒性的研究[J].黑龙江农业科学,2014,(10):65.
SONG Yan-dong,LIU Qing,ZHAO Heng-shou.Research on Acute Toxicity of Caulis spatholobi and Radix glycyrrhizae to Goldfish and Loach[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):65.
[10]崔瑞峰,张丽霞,韩晓红.不同激素配比对百合鳞片愈伤组织诱导的影响[J].黑龙江农业科学,2014,(09):8.
CUI Rui-feng,ZHANG Li-xia,HAN Xiao-hong.Effect of Different Hormone Combinations on Callus Induction Frequency of Lily Scale[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):8.
备注/Memo
收稿日期：2021-05-06