[1]于昕,王玉晗,薛腾,等.抗生素对马铃薯甲虫嗅觉基因的影响[J].黑龙江农业科学,2021,(04):49-54.[doi:10.11942/j.issn1002-2767.2021.04.0049]
　YU Xin,WANG Yu-han,XUE Teng,et al.Effects of Antibiotics on Olfactory Genes of Leptinotarsa decemlineata[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(04):49-54.[doi:10.11942/j.issn1002-2767.2021.04.0049]
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抗生素对马铃薯甲虫嗅觉基因的影响

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2021年04 页码: 49-54 栏目: 出版日期: 2021-04-10
Title:
Effects of Antibiotics on Olfactory Genes of Leptinotarsa decemlineata
作者:
于昕1; 2; 王玉晗3; 薛腾4; 李红卫1; 郑超5; 石旺鹏2; 于艳雪1
1.中国检验检疫科学研究院 植物检疫研究所，北京 100176； 2.中国农业大学 植物保护学院，北京 100193；3.重庆微浪生物科技有限公司，重庆 400021；4.中华人民共和国大连海关，辽宁 大连 116000；5.哈尔滨海关技术中心，黑龙江 哈尔滨 150000
Author(s):
YU Xin1; 2; WANG Yu-han3; XUE Teng4; LI Hong-wei1; ZHENG Chao5; SHI Wang-peng2; YU Yan-xue1
1.Institute of Plant Quarantine，Chinese Academy of Inspection and Quarantine，Beijing 100176，China; 2.Institute of Plant Protection,China Agricultural University,Beijing 100193,China; 3.Chongqing BioWavelet Technology Limited,Chongqing 400021,China; 4.Dalian Customs of the People’s Republic of China,Dalian 116000,China; 5.Harbin Customs Technology Center,Harbin 150000,China
关键词:
马铃薯甲虫; 嗅觉基因; 肠道微生物; 抗生素
Keywords:
Leptinotarsa decemlineata (say);  olfactory genes;  intestinal microorganisms;  antibiotics
DOI:
10.11942/j.issn1002-2767.2021.04.0049
文献标志码:
A
摘要:
为促进马铃薯甲虫嗅觉行为及生物防治研究，本文对马铃薯甲虫成虫饲喂抗生素后，利用转录组测序探究健康和取食抗生素后成虫头部的嗅觉基因差异。结果表明：通过Illumina HiseqTM测序和差异基因的筛选富集，发现饲喂抗生素后，在代谢途径上的相关基因显著上调（q-value≤0.05），并且筛选出4种差异显著的气味分子结合蛋白（OBP），分别是LdecOBP6、LdecOBP11、LdecOBP26和LdecOBP28；利用实时荧光PCR和数字PCR验证了健康与饲喂抗生素的嗅觉基因表达量的变化，饲喂抗生素后LdecOBP6、LdecOBP11、LdecOBP26和LdecOBP28这4种基因的表达量确有显著变化。
Abstract:
In order to promote the study on olfactory behavior and biological control of potato beetles,after the potato beetle being fed with antibiotics,we used transcriptome sequencing to explore the differences of olfactory genes in the heads of healthy potato beetles and those fed antibiotics.The results showed that through Illumina Hiseq sequencing and screening of differential genes,we found that the related genes in the metabolic pathway were significantly up-regulated (q-value≤0.05) after feeding antibiotics.Besides,there were 4 types of odorant binding proteins (OBP) with significant differences were also screened,which were LdecOBP6,LdecOBP11,LdecOBP26 and LdecOBP28.Real time PCR and digital PCR were used to verify the changes of olfactory gene expression in healthy and treated groups.There were significant changes in the expression levels of the four genes,LdecOBP 6,LdecOBP 11,LdecOBP 26 and LdecOBP 28 after feeding antibiotics.

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备注/Memo

收稿日期：2020-12-15

基金项目：中国检验检疫科学研究院基本科研业务费项目（2019JK021)。
第一作者：于昕（1994-），女，在读硕士，从事农业昆虫与害虫防治研究。E-mail：yuxin19940604@163.com。
通信作者：于艳雪（1978-），女，博士，副研究员，从事检疫性昆虫监测技术及昆虫化学生态学和嗅觉分子机理研究。E-mail：yuyx@caiq.org.cn。

更新日期/Last Update: 2021-04-22