[1]杨焕春,李勇,单宏,等.黑龙江省粳稻田间与储藏真菌群落演替规律研究[J].黑龙江农业科学,2020,(12):86-92.[doi:10.11942/j.issn1002-2767.2020.12.0086]
　YANG Huan-chun,LI Yong,SHAN Hong,et al.Study on Field and Storage Fungal Community Diversity in Japonica Rice of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(12):86-92.[doi:10.11942/j.issn1002-2767.2020.12.0086]
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黑龙江省粳稻田间与储藏真菌群落演替规律研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2020年12期 页码: 86-92 栏目: 出版日期: 2020-12-10
Title:
Study on Field and Storage Fungal Community Diversity in Japonica Rice of Heilongjiang Province
作者:
杨焕春1; 李勇1; 单宏2; 李莉莉2; 兰静2; 赵琳2; 孙向东2
1.安顺学院，贵州 安顺 561000；2.黑龙江省农业科学院 农产品质量安全研究所，黑龙江 哈尔滨 150086
Author(s):
YANG Huan-chun1; LI Yong1; SHAN Hong2; LI Li-li2; LAN Jing2; ZHAO Lin2; SUN Xiang-dong2
1.Anshun University，Anshun 561000，China；2.Quality and Safety Institute of Agricultural Products,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China
关键词:
粳稻; 田间真菌; 群落构成; 多样性; 功能预测
Keywords:
japonica rice;  field fungi;  community structure;  diversity;  functional prediction
DOI:
10.11942/j.issn1002-2767.2020.12.0086
文献标志码:
A
摘要:
为促进黑龙江省稻谷合理仓储，保障黑龙江省粳稻品质安全，采用Illumina Hiseq高通量测序方法，于2016年秋季在黑龙江省五常地区分别采收3份新鲜水稻样品、将这些样品仓储1，2和3 a后进行ITS区的高通量测序，分析了物种组成和丰度、Alpha多样性和菌群结构。结果表明：入库储藏3 a后，稻谷样品储藏真菌群落构成演化为子囊菌门（Ascomycota）仍占绝对优势，但比重由94.4%下降为82.1%；散囊菌目（Eurotiales,25.6%)和煤炱目（Capnodiales,20.1%)为优势目；曲霉属（Aspergillus,24.7%)和节担菌属（Wallemia,7.4%)为优势属；帚状曲霉（Aspergillus penicillioides,14.3%)为优势种。不同储藏时间的水稻样品真菌物种组成丰富,真菌种群结构存在一定差异。
Abstract:
In order to promote reasonable storage and quality safety of paddy rice in Heilongjiang Province,three freshly harvested rice samples were collected in 2016 and stored for 1,2,and 3 years,then analyzed through high-throughput sequencing of Illumina Hiseq to investigate the composition of storage fungal community.These rice samples were conducted high-throughput sequencing of ITS region,species composition,abundance,alpha diversity,and community structure were also analyzed.The results indicated that through three years storage,the storage fungal community in rice samples changed to as follow: Ascomycota was still the absolute predominant phylum,however the proportion declined from 94.4% to 82.1%; Eurotiales(25.6%) and Capnodiales (20.1%) were the predominant order;Aspergillus(24.7%) and Wallemia(7.4%) were the predominant genus;Aspergillus penicillioides(14.3%) was the predominant specie.Fungal species composition of rice samples stored for different times were abundant and differences of fungal composition structure existed among these rice samples.

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备注/Memo

收稿日期：2020-07-24

基金项目：哈尔滨市优秀学科带头人基金资助项目（2013RFXYJ049,2016RAXYJ085）；国家农产品质量安全风险评估重大专项（GJFP2019016）；安顺学院博士基金（Asxybsjj201901）。
第一作者：杨焕春（1978-），女，硕士，实验师，从事食品质量安全研究。E-mail：541557434@qq.com。
通信简介：孙向东（1965-），男，博士，研究员，从事农产品质量安全风险评估研究。E-mail: xdsun65@yahoo.com。

更新日期/Last Update: 2020-12-25