[1]朱立新,冯华健,李鑫,等.紫叶稠李组织培养与快速繁殖[J].黑龙江农业科学,2019,(10):15-17.[doi:10.11942/j.issn1002-2767.2019.10.0015]
　ZHU Li-xin,FENG Hua-jian,LI Xin,et al.Tissue Culture and Rapid Propagation of Padus virginiana[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(10):15-17.[doi:10.11942/j.issn1002-2767.2019.10.0015]
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紫叶稠李组织培养与快速繁殖

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年10期 页码: 15-17 栏目: 出版日期: 2019-10-10
Title:
Tissue Culture and Rapid Propagation of Padus virginiana
作者:
朱立新; 冯华健; 李鑫; 刘敏; 梁艳; 徐洪国
齐齐哈尔大学 生命科学与农林学院/抗性基因工程与寒地生物多样性保护黑龙江省重点实验室，黑龙江 齐齐哈尔 161006
Author(s):
ZHU Li-xin; FENG Hua-jian; LI Xin; LIU Min; LIANG Yan; XU Hong-guo
College of Life Sciences and Agriculture and Forestry,Qiqihar University,Key Laboratory of Resistance Gene Engineering and Cold Biodiversity Conservation,Qiqihar 161006,China
关键词:
紫叶稠李; 组织培养; 快速繁殖
Keywords:
Padus virginiana; tissue culture; rapid propogation
DOI:
10.11942/j.issn1002-2767.2019.10.0015
文献标志码:
A
摘要:
为建立紫叶稠李组织培养快速繁殖方法，对紫叶稠李重要种质材料的保存扩繁提供技术支持。本研究以紫叶稠李为材料，以MS培养基为基本培养基，通过不同种类和浓度的植物生长调节剂配比试验，筛选紫叶稠李快速繁殖与生根的最佳培养基组成。结果表明：紫叶稠李试管苗最佳增殖培养基为MS+2~3 mg?L-1 6-BA+0.1 mg?L-1 NAA+30 g?L-1 蔗糖+8 g?L-1 琼脂，最佳生根培养基为1/2 MS+0.1 mg?L-1 IBA+30 g?L-1蔗糖+8 g?L-1琼脂，生根诱导20 d，生根率达90％。将生根的试管苗在室外进行炼苗后，移栽到营养钵中，成活率达到90％以上。
Abstract:
In order to establish a rapid propagation method for tissue culture of Padus virginiana,technical support was provided for the preservation and propagation of important germplasm materials of Padus virginiana.In this study,Padus virginiana was used as material,MS medium was used as basic medium,and the optimum medium composition for rapid propagation and rooting of Padus virginiana was screened through the ratio test of plant growth regulators of different kinds and concentrations.The results showed that the optimum medium for multiplication was MS+2-3 mg?L-16-BA+0.1 mg?L-1 NAA+30 g?L-1 sucrose+8 g?L-1 agar,and the optimum medium for rooting was 1/2 MS+0.1 mg?L-1 IBA+30 g?L-1 sucrose+8 g?L-1 agar.Rooting induction lasted for 20 days and the rooting rate reached 90%.After the rooting test-tube seedlings were refined outdoors,they were transplanted into nutrient bowls,and the survival rate reached more than 90%.

参考文献/References:

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备注/Memo

收稿日期：2019-04-01

基金项目：2018年齐齐哈尔大学大学生创新创业训练计划（201810232147）；黑龙江省省属高等学校基本科研业务项目（135109252、135109256）；黑龙江省自然科学基金项目（C2018063）。
第一作者简介：朱立新（1998-），男，在读学士，专业为园艺。E-mail：1362559285@qq.com。
通讯作者：徐洪国（1977-），男，博士，副教授，从事园艺植物生物技术研究。E-mail：xuhongguo1977@163.com。

更新日期/Last Update: 2019-10-25