[1]来艳华,赵亚中.玉米蔗糖合成酶基因Sh1生物信息学分析[J].黑龙江农业科学,2019,(10):4-9,10.[doi:10.11942/j.issn1002-2767.2019.10.0004]
　LAI Yan-hua,ZHAO Ya-zhong.Bioinformatics Analysis on Sh1Gene of Maize Sucrose Synthase[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(10):4-9,10.[doi:10.11942/j.issn1002-2767.2019.10.0004]
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玉米蔗糖合成酶基因Sh1生物信息学分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年10期 页码: 4-9,10 栏目: 出版日期: 2019-10-10
Title:
Bioinformatics Analysis on Sh1Gene of Maize Sucrose Synthase
作者:
来艳华; 赵亚中
黑龙江省农业综合开发设计所，黑龙江 哈尔滨 150040
Author(s):
LAI Yan-hua; ZHAO Ya-zhong
Heilongjiang Agriculture Development Design Institute,Harbin 150040,China
关键词:
玉米; 蔗糖合成酶; Sh1基因
Keywords:
maize;  sucrose synthase;  Sh1 gene
DOI:
10.11942/j.issn1002-2767.2019.10.0004
文献标志码:
A
摘要:
蔗糖合成酶是玉米生物合成及代谢途径实现蔗糖合成和分解的关键酶，蔗糖合成酶基因Sh1位于玉米第9号染色体的短臂，基因全长5 816 bp。Sh1编码蛋白SH1含802个氨基酸，由1个蔗糖合成酶亚基（PF00862）和1个糖基转移酶亚基（PF00534）组成，是一种具有催化合成和分解双重作用的可逆酶，不存在跨膜结构，属于稳定型膜外蛋白，3种可能的结构模型显示其具有复杂的空间结构，玉米籽粒蔗糖生物合成的关键时期是授粉后12~27 d。生物进化结果显示，玉米和高粱、甘蔗的蔗糖合成酶基因Sh1在物种进化演变中序列高度保守。为揭示玉米蔗糖代谢途径的分子机制，本研究对Sh1基因结构及功能进行了分析，并对Sh1编码蛋白结构进行了基本理化性质和物种进化分析。
Abstract:
Sucrose synthase is a key enzyme in the biosynthesis and metabolic pathway of maize to achieve sucrose synthesis and decomposition.Sucrose synthase gene Sh1 is located in the short arm of chromosome 9 of maize,with the total length of 5 816 bp.Sh1-encoded protein SH1,containing a sucrose synthetase subunit(PF00862) and a glycosyl transferase subunit (PF00534) with 802 amino acids,was a combination of reversible enzyme catalytic synthesis and decomposition,which belongs to the stable type of membrane protein without transmembrane structure,and the three possible model shows that it has the complicated space structure.The key period of sucrose biosynthesis in maize is 12-27 days after pollination.In order to reveal the molecular mechanism of sucrose metabolism pathway in maize,the structure and function of Sh1 gene were analyzed,and the basic physical and chemical properties and species evolution of Sh1encoding protein structure were studied.

参考文献/References:

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备注/Memo

收稿日期：2019-04-17

第一作者简介：来艳华（1980-），女，硕士，高级农艺师，从事农业开发项目管理工作。E-mail：15004508409@163.com。

更新日期/Last Update: 2019-10-25