[1]韩晓云,郑桂华,郑宝江,等. Pseudomonas sp.H8的分离和鉴定及其产絮凝剂成分分析[J].黑龙江农业科学,2019,(09):115-120.[doi:10.11942/j.issn1002-2767.2019.09.0115]
　HAN Xiao-yun-,ZHENG Gui-hua-,ZHENG Bao-jiang-,et al.Isolation and Identification of Pseudomonas sp.H8 with Flocculant-producing Capacity and Analysis of the Flocculant[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(09):115-120.[doi:10.11942/j.issn1002-2767.2019.09.0115]
点击复制

Pseudomonas sp.H8的分离和鉴定及其产絮凝剂成分分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年09期 页码: 115-120 栏目: 出版日期: 2019-09-10
Title:
Isolation and Identification of Pseudomonas sp.H8 with Flocculant-producing Capacity and Analysis of the Flocculant
作者:
韩晓云1; 2; 郑桂华1; 2; 郑宝江3; 王运来1; 2; 刘杨1; 2; 康传红1; 2; 李秀凉1; 2; 孙庆申1; 2
1.黑龙江大学 农业微生物技术教育部工程研究中心，黑龙江 哈尔滨 150500；2.黑龙江大学 生命科学学院/黑龙江省普通高等学校微生物重点实验室，黑龙江 哈尔滨 150080；3.东北林业大学 生命科学学院，黑龙江 哈尔滨 150040
Author(s):
HAN Xiao-yun1; 2-; ZHENG Gui-hua1; 2-; ZHENG Bao-jiang3-; WANG Yun-lai1; 2-; LIU Yang1; 2-; KANG Chuan-hong1; 2-; LI Xiu-liang1; 2-; SUN Qing-shen1; 2-
1.Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500,China
关键词:
低温絮凝菌筛选; 菌种鉴定; H8絮凝剂; 成分分析
Keywords:
low temperature flocculants-producing bacteria screening;  strain identification;  strain H8-derived flocculants;  component analysis
DOI:
10.11942/j.issn1002-2767.2019.09.0115
文献标志码:
A
摘要:
为挖掘产生物絮凝剂的菌株，利用生物絮凝剂对污水、淤泥进行环保处理。本研究经过菌种分离纯化和对高岭土悬浊液沉降效果分析，从低温污水处理生物膜中获得1株高效絮凝菌，命名为H8，该菌株在4 ℃条件下生长良好，其LB发酵液对高岭土的絮凝效率达到85.79%，针对H8菌株的一系列菌种鉴定，包括菌落形态、菌体形态、生理生化试验、Biolog菌种鉴定系统分析和细菌16S rDNA序列分析比对，与菌种Pseudomonas mandelii亲缘关系最近，确定H8菌株为假单胞菌属。采用乙醇沉淀法对H8菌株发酵液中的絮凝剂进行提取，再经过Sevage法除去絮凝剂中蛋白质，获得絮凝剂产率0.807 g·L-1-，与发酵液相比其絮凝率也提高到98%以上，定性定量分析结果确定H8絮凝剂的化学成分主要为多糖，占51%，还含有2.64%蛋白质、1.3%-葡萄糖醛酸和少量氨基糖。
Abstract:
The use of bioflocculants for the treatment of sewage and sludge has such advantages as environmentally friendly and no secondary pollution.Therefore,the exploitation of flocculants-producing strains,especially those that produce flocculants at low temperature,has important significance theoretically and application prospects.In this study,the strain named H8 with efficient flocculation activities was isolated and purified from the low-temperature sewage treatment biofilm,the flocculation effect towards kaolin suspension was also evaluated.The strain grew well at 4 ℃,and its LB fermentation broth showed 85.79% flocculation efficiency towards kaolin.The H8 strain was characterized with colony and cell morphology,physiological and biochemical tests,Biolog strain identification system analysis and bacterial 16S rDNA sequence analysis,which showed close relation to Pseudomonas mandelii and identified as Pseudomonas.The flocculants in the H8 strain-derived fermentation broth was extracted by ethanol precipitation with flocculants yield of 0.807 g·L-1- after the protein removal by sevage method.The flocculation rate was also increased to 98%.Qualitative and quantitative analysis confirmed that the chemical constituents of H8 flocculants were mainly composed of polysaccharides which accounted for 51% (w/w),protein (2.64%,w/w),glucuronic acid (1.3%,w/w) and a small amount of amino sugar.

参考文献/References:

［1］-张一卉,朱迟,梁慧芳,等.微生物絮凝剂概况及发展前景[J].-生命科学研究,2005(S1):39-42.

[2]Yang P,Li D,Zhang W,et al.Flocculation-dewatering behavior of waste activated sludge particles under chemical conditioning with inorganic polymer flocculant: Effects of typical sludge properties[J].Chemosphere,2019,218:930-940.
[3]李梦茜,莫松柏,钱荣坤.微生物絮凝剂应用于污水处理的研究进展[J].大众科技,2019,21(4):30-32.
[4]李春玲,金若菲,周集体,等.利用污泥残渣制备生物絮凝剂及其絮凝机理的探讨[J].环境科学与技术，2012，35(1)：38-41，94.
[5]任敦建,宋汕柯,李红阳,等.利用木薯淀粉酒精废水培养复合型生物絮凝产生菌条件优化及其应用研究[J].广东农业科学，2013，40(10)：97-100.
[6]易小畅,蒋智,陆兵,等.糖蜜酒精废液培养微生物絮凝剂产生菌的研究[J].酿酒科技，2014(1)：85-88.
[7]Merkel- T,Blattler O,Konigsson S.Flocculant screening method at lab scale for application in disc stack centrifuges with Hermetic Design[J].Chemical Engineering & Technology，2018，41(12)：2312-2322.
[8]刘泽群.低温复合产絮菌去除铁离子的试验研究[D].哈尔滨：黑龙江大学,2010.
[9]Shi C,Sun W,Sun Y,et al.Synthesis,characterization,and sludge dewaterability evaluation of the chitosan-based flocculant CCPAD[J].Polymers(Basel)，2019，11(1)：E95.DOI:10.3390/polym11010095.
[10]Wang Z,Huang W,Yang G,et al.Preparation of cellulose-base amphoteric flocculant and its application in the treatment of wastewater[J].Carbohydrate Polymers,2019,215:179-188.
[11]沈萍,范秀荣,李广武.微生物学实验[M].4版.北京:高等教育出版社,2007.
[12]〖JP2〗Yoon J H,Cho Y G,Kang S S,et al.Rhodococcus koreensis sp.nov.,a 2,4-dinitrophenol-degrading bacterium[J].〖JP〗International Journal of Systematic and Evolutionary Microbiology,2000,50:1193-201.

备注/Memo

收稿日期：2019-04-17

基金项目：黑龙江省教育厅面上项目（12521410）。
第一作者简介：韩晓云(1970-)，女，博士，副教授，从事环境微生物、食品和药物物质功能的挖掘及开发研究。E-mail: hanxiaoyun70@126.com。
通讯作者：孙庆申（1977-），男，博士，教授，从事食品和药物物质功能的挖掘及开发研究。E-mail: sunqingshen@hlju.edu.cn。

更新日期/Last Update: 2019-09-17