[1]刘建新,吴立成,王冠,等.黑龙江省一、二积温区水稻花药培养条件优化[J].黑龙江农业科学,2019,(08):9-14.[doi:10.11942/j.issn1002-2767.2019.08.0009]
　LIU Jian-xin,WU Li-cheng,WANG Guan,et al.Conditional Optimization of Rice Anther Culture in First and Second Accumulated Temperature Zones of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(08):9-14.[doi:10.11942/j.issn1002-2767.2019.08.0009]
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黑龙江省一、二积温区水稻花药培养条件优化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年08期 页码: 9-14 栏目: 出版日期: 2019-08-10
Title:
Conditional Optimization of Rice Anther Culture in First and Second Accumulated Temperature Zones of Heilongjiang Province
作者:
刘建新1; 2; 吴立成2; 3; 王冠4; 李希臣1; 2; 李炜1; 2; 刘明1; 2; 刘淼1; 2; 王玲1; 2
1.黑龙江省农业科学院 耕作栽培研究所，黑龙江 哈尔滨 150086；2.黑龙江省作物分子设计与种质创新重点实验室,黑龙江 哈尔滨 150086；3.黑龙江省农业科学院 生物技术研究所，黑龙江 哈尔滨 150086； 4.黑龙江省农业科学院 黑龙江农业科技杂志社，黑龙江 哈尔滨 150086
Author(s):
LIU Jian-xin1; 2; WU Li-cheng2; 3; WANG Guan4; LI Xi-chen1; 2; LI Wei1; 2; LIU Ming1; 2; LIU Miao1; 2; WANG Ling1; 2
1.Institute of Crop Cultivation and Tillage,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China; 2.Heilongjiang Provincial Key Laboratory of Crop Molecular Design and Germplasm Innovation,Harbin 150086,China; 3.Biotechnology Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086,China；4.Heilongjiang Journal Press of Agricultural Science and Technology,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China
关键词:
水稻; 花药培养效率; 条件优化
Keywords:
rice;  anther culture efficiency;  optimization
DOI:
10.11942/j.issn1002-2767.2019.08.0009
文献标志码:
A
摘要:
为提高黑龙江省第一、二积温带水稻花药培养效率，促进该技术在粳稻新品种选育中的应用，以五优稻2号及黑龙江省第一、二积温区骨干亲本的F1、F2为材料，对提高花培效率的几个关键环节如取材标准、关键时期培养基配方和培养条件等进行了方法改良。结果表明：取穗以叶枕距、颖壳颜色和花药长度等多重因素为标准；适用于以五优稻2号为亲本的诱导培养基配方为进口N6+凝胶3.6 g·L-1+蔗糖60 g·L-1+2,4-D 2 mg·L-1；分化培养基配方为MS+KT 2 mg·L-1+IAA 1 mg·L-1 +蔗糖30 g·L-1 +琼脂4 g·L-1，同时辅以16 h光照；生根培养基中加入3~6 mg〖DK1〗·L-1多效唑，可以壮苗促分蘖，提高移栽成活率。
Abstract:
In order to improve the anther culture efficiency of rice and promote the application of the technology in the breeding of new japonica rice varieties in Heilongjiang Province,especially in the first and second accumulative temperate zones,in this paper,we used Wuyoudo,F1 and F2 of backbone parents in the first and second accumulated temperature zonse of Heilanyjiang Province as materials,and imqroved several key links of rice anther culture,such as the selection criteria,medium formulation and culture conditions in the critical period.The results showed that the multiple criteria of choosing rice spikes:the color of glume,anther length and length of pulvinus; Formula of induction media suitable for Wuyoudao 2: imported N6+phytagel 3.6 g·L-1+sugar 60 g·L-1+2,4-D 2 mg·L-1;Formula of regenerate media: MS+KT 2 mg·L-1+IAA 1 mg·L-1+sugar 30 g·L-1+agar 4 g·L-1,simultaneously supplemented by 16 h illumination;3-6 mg·L-1 MET in rooting media is good for tillering and improving transplant survival rate.

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备注/Memo

收稿日期：2019-03-15

基金项目：黑龙江省农业科技创新工程院级重点项目（2014ZD032）；黑龙江省应用技术研究与开发计划重大项目（GA18B101）。
第一作者简介：刘建新（1976-），女，博士，助理研究员，从事分子生物学研究。E-mail：wendyliujx@163.com。

更新日期/Last Update: 2019-09-12