[1]杨运良,李建勋,丁宵,等.改良CTAB法提取红肉火龙果果实不同组织总DNA及质量检测[J].黑龙江农业科学,2019,(07):36-38.[doi:10.11942/j.issn1002-2767.2019.07.0036]
　YANG Yun-liang,LI Jian-xun,DING Xiao,et al.Extraction and Quality Detection of Total DNA from Different Tissues of Red Dragon Fruit by Modified CTAB Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(07):36-38.[doi:10.11942/j.issn1002-2767.2019.07.0036]
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改良CTAB法提取红肉火龙果果实不同组织总DNA及质量检测

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年07期 页码: 36-38 栏目: 出版日期: 2019-07-10
Title:
Extraction and Quality Detection of Total DNA from Different Tissues of Red Dragon Fruit by Modified CTAB Method
作者:
杨运良; 李建勋; 丁宵;  郭创业; 董少鹏; 段国琪; 武宗信
山西省农业科学院 棉花研究所，山西 运城 044000
Author(s):
YANG Yun-liang; LI Jian-xun; DING Xiao; GUO Chuang-ye; DONG Shao-peng; DUAN Guo-qi; WU Zong-xin
Cotton Research Institute,Shanxi Academy of Agricultural Sciences,Yuncheng 044000,China
关键词:
红肉火龙果; 总DNA; 不同组织
Keywords:
dragon fruit;  total DNA;  different tissues
DOI:
10.11942/j.issn1002-2767.2019.07.0036
文献标志码:
A
摘要:
为探求红肉火龙果果实不同组织DNA含量情况，本研究以成熟期红肉火龙果果实为材料，采用改良CTAB法对火龙果果肉、果皮、种子进行总DNA提取。结果表明：红肉火龙果果肉、果皮、种子的OD260/OD280光吸收值分别为1.982 7，1.984 4，1.997 5，均在1.8≤OD260/OD280<2.0，说明DNA纯度均很好。同一红肉火龙果果实不同组织中DNA浓度各不相同，其中种子中的DNA浓度为0.135 μg·mL-1，含量最高，其次果皮中的DNA浓度0.078 μg·mL-1，果肉中DNA浓度最低，为0.036 μg·mL-1。
Abstract:
In order to explore the content of DNA in different tissues of red dragon fruit,the total DNA of flesh,pericarp and seed of dragon fruit was extracted by modified CTAB method.The results showed that the OD260/OD280 light absorption values of pulp,peel and seed were 1.982 7,1.984 4 and 1.997 5 respectively,all of which were between 1.8≤OD260/OD280 ≤2.0,indication that the purity of DNA was very good.The concentration of DNA in different tissues of the same red flesh dragon fruit was different,in which the concentration of DNA in seed was 0.135 μg·mL-1,and the concentration of DNA in pericarp was 0.078 μg·mL-1,the concentration of DNA in pulp was the lowest,0.036 μg·mL-1.

参考文献/References:

［1］邓仁菊,范建新,蔡永强.国内外火龙果研究进展及产业发展现状 [J].贵州农业科学,2011,39(6): 188-192.

[2]朱英,陶刚,刘作易,等.火龙果基因组DNA提取的新方法[J].问题探讨,2008,27(11): 111-112.
[3]余志雄,欧高政,陈清西,等.火龙果DNA提取方法比较研究[J].中国农学通报,2010,26(4): 300-303.
[4]韦茜,蔡永强,金吉芬,等.CTAB法提取火龙果基因组DNA的试验研究 [J].安徽农业科学,2008,36(13): 5325-5326.

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[1]杨运良,李建勋,郭创业,等.运城地区红肉火龙果初步筛选及日光温室关键栽培技术[J].黑龙江农业科学,2019,(10):70.[doi:10.11942/j.issn1002-2767.2019.10.0070]
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备注/Memo

收稿日期：2019-02-23

基金项目：山西省科技厅面上青年基金项目（201701D221204）；山西省农科院特色农业技术攻关项目（YGG17050）。
第一作者简介：杨运良（1986-），男，硕士，助理研究员，从事设施果树栽培及生理生化研究。E-mail：yyl_7514@163.com。

更新日期/Last Update: 2019-07-09