[1]赫卫,张慧,王莹.辣椒种质资源的形态学和SRAP分析[J].黑龙江农业科学,2019,(05):7-12.[doi:10.11942/j.issn1002-2767.2019.05.0007]
　HE Wei,ZHANG Hui,WANG Ying.Analysis of Morphology and SRAP of Pepper Germplasm Resources[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(05):7-12.[doi:10.11942/j.issn1002-2767.2019.05.0007]
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辣椒种质资源的形态学和SRAP分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年05期 页码: 7-12 栏目: 出版日期: 2019-05-10
Title:
Analysis of Morphology and SRAP of Pepper Germplasm Resources
作者:
赫卫; 张慧; 王莹
黑龙江省农业科学院 园艺分院，黑龙江 哈尔滨 150069
Author(s):
HE Wei;  ZHANG Hui;  WANG Ying
Institute of Horticulture，Heilongjiang Academy of Agricultural Sciences，Harbin 150069,China
关键词:
辣椒; 形态学; SRAP; 聚类分析
Keywords:
Capsicum annuum L.;  morphology;  SRAP;  cluster analysis
DOI:
10.11942/j.issn1002-2767.2019.05.0007
文献标志码:
A
摘要:
为探索辣椒种质的遗传多样性和亲缘关系，对43份辣椒种质进行形态学和SRAP分子标记分析。结果表明：根据辣椒的28个形态学性状进行聚类分析，在遗传距离系数6处可以聚为6个组群。94对SRAP引物组合中，每对引物平均扩增的清晰条带数为8.34条，其中多态性比率为13.90%， 从中筛选出28对多态性丰富的引物用于SRAP分析。 绘制亲缘关系聚类图，43份辣椒种质间的相似系数分布为0.59～0.96，在0.72的相似系数处分为6个组群。SRAP聚类与形态学聚类相似度高，二者都可为辣椒的遗传分析及育种亲本的选择提供一定的借鉴作用。
Abstract:
In order to explore the genetic diversity and genetic relationship of pepper germplasms,43 pepper germplasms were analyzed by morphology and SRAP molecular markers.The results showed that cluster analysis based on 28 morphological characters of pepper could be clustered into 6 groups at 6 genetic distance coefficients.Among 94 pairs of SRAP primer combinations,the average number of clear bands amplified by each pair of primers was 8.34,and the polymorphism ratio was 13.90%.28 pairs of primers with rich polymorphism were selected for SRAP analysis.The similarity coefficient of 43 pepper germplasms ranged from 0.59 to 0.96,and divided into 6 groups at 0.72 similarity coefficient.SRAP clustering and morphological clustering have high similarity,both of them can provide some reference for genetic analysis and selection of breeding parents of pepper.

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备注/Memo

收稿日期：2018-11-26

基金项目：〖JP2〗现代农业产业技术体系建设专项资金资助(CARS-24-G-04)；黑龙江省农业科学院园艺分院青年人才项目。〖JP〗
第一作者简介：赫卫（1981-），女，博士，助理研究员，从事辣椒遗传育种研究。E-mail：la_jiao800@163.com。
通讯作者：张慧（1971-），女，硕士，副研究员，从事辣椒育种研究。E-mail:la_jiao8000@163.com。

更新日期/Last Update: 2019-06-12