[1]王玉珍,蔡进宇,李振,等.烟草PPO氨基酸序列分析[J].黑龙江农业科学,2019,(04):18-21.[doi:10.11942/j.issn10022767.2019.04.0018]
　WANG Yuzhen,CAI Jinyu,LI Zhen,et al.Analysis of Amino Acid Sequence of Tobacco PPO[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(04):18-21.[doi:10.11942/j.issn10022767.2019.04.0018]
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烟草PPO氨基酸序列分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年04期 页码: 18-21 栏目: 出版日期: 2019-04-10
Title:
Analysis of Amino Acid Sequence of Tobacco PPO
作者:
王玉珍1; 蔡进宇2; 李振3; 吴杰4; 杨志孝2; 苏家恩5
1.大理州烟草公司 云龙县分公司，云南 云龙 672700；2.大理州烟草公司 大理市分公司，云南 大理 671000；3.大理州烟草公司 漾濞县分公司，云南 漾濞 672500；4.大理州烟草公司 洱源县分公司，云南 洱源 671200；5.云南省烟草公司 大理州公司，云南 大理 671000
Author(s):
WANG Yuzhen1; CAI Jinyu2; LI Zhen3; WU Jie4; YANG Zhixiao2; SU Jiaen5
1.Yunlong County Branch Company,Dali Prefecture Tobacco Company,Yunlong 672700,China; 2.Dali Branch of Dali Prefecture Tobacco Company,Dali 671000,China; 3.Yangbi County Branch Company,Dali Prefecture Tobacco Company,Yangbi 672500,China; 4.Eryuan Branch of Dali Prefecture Tobacco Company,Eryuan 671200,China; 5.Dali Prefecture Tobacco Company,Yunnan Tobacco Company,Dali 671000,China
关键词:
烟草; PPO氨基酸; 序列
Keywords:
tobacco;  PPO amino acid;  sequence
DOI:
10.11942/j.issn10022767.2019.04.0018
文献标志码:
A
摘要:
为研究烟草PPO的理化特性，采用PROTPARAM、SMART和NPSAPRABI工具解析烟草PPO成员的生理特性、结构域和二级结构。结果表明：烟草PPO为酸性氨基酸，NtPPO1、NtPPO2和NtPPO3属于不稳定的蛋白，其中以NtPPO1的耐热性相对较强，三者共同含量较高的氨基酸为亮氨酸和脯氨酸；NtPPO1、NtPPO2和NtPPO3均含有Tyrosinase、PPO1_DWL和PPO1_KFDV结构域，且NtPPO1和NtPPO2含有信号肽；烟草PPO成员的二级结构主要为无规则卷曲，其次是α螺旋，各二级结构排列较为分散。
Abstract:
In order to study the physicochemical properties of tobacco PPO,the physiological characteristics,domain and secondary structure of tobacco PPO members were analyzed by PROTPARAM,SMART and NPSAPRABI tools.The results showed that tobacco PPO was acidic amino acid,NtPPO1,NtPPO2 and NtPPO3 belonged to unstable protein,among which NtPPO1 had relatively strong heat resistance,and the three amino acids with higher common content were leucine and valine; NtPPO1 both NtPPO2 and NtPPO3 contain Tyrosinase,PPO1_DWL and PPO1_KFDV domains,and NtPPO1 and NtPPO2 contain signal peptides; the secondary structure of tobacco PPO members was mainly irregular coiling,followed by αhelix,and the secondary structure was more dispersed.

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备注/Memo

收稿日期：20181119

第一作者简介：王玉珍（1975），助理农艺师，从事烟叶生产技术研究指导。Email：705763042@qq.com。
通讯作者：苏家恩（1976），男，硕士，高级农艺师，从事烟叶烘烤技术及烤房研究和推广工作。Email：dlyc8816@163.com。

更新日期/Last Update: 2019-05-08