[1]苏玲,王鹏飞,杨阳,等.葡萄全基因组WRKY转录因子鉴定和分析[J].黑龙江农业科学,2019,(01):13-22.[doi:10.11942/j.issn1002-2767.2019.01.0013]
　SU Ling,WANG Peng-fei,YANG Yang,et al.Genome-wide Identification and Analysis of WRKY Transcription Factors in Grape[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(01):13-22.[doi:10.11942/j.issn1002-2767.2019.01.0013]
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葡萄全基因组WRKY转录因子鉴定和分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年01期 页码: 13-22 栏目: 出版日期: 2019-01-10
Title:
Genome-wide Identification and Analysis of WRKY Transcription Factors in Grape
作者:
苏玲; 王鹏飞; 杨阳;  任凤山; 王咏梅; 陈万钧
山东省葡萄研究院/山东省葡萄栽培与深加工工程技术研究中心/农业部华东都市农业重点实验室,山东 济南 250100
Author(s):
SU Ling; WANG Peng-fei; YANG Yang; REN Feng-shan; WANG Yong-mei; CHEN Wan-jun
Shandong Academy of Grape,Shandong Engineering Research Center for Grape Cultivation and Deep-processing,MOA Key Laboratory of East China Urban Agriculture,Jinan 250100,China
关键词:
葡萄; WRKY; 转录因子; 进化分析; 表达分析
Keywords:
grape;  WRKY;  transcription factor; phylogenetic analysis; expression analysis
DOI:
10.11942/j.issn1002-2767.2019.01.0013
文献标志码:
A
摘要:
为鉴定和分析葡萄全基因组中WRKY家族转录因子基因，利用生物信息学的方法，分析葡萄全基因组WRKY转录因子类型、进化分析、染色体定位、基因结构、保守元件和基因表达模式。结果表明：葡萄全基因组中有56个WRKY转录因子。序列比对和系谱进化表明，分为Ⅰ、Ⅱ和Ⅲ三个组，Ⅱ组分为Ⅱ-a、Ⅱ-b、Ⅱ-c、Ⅱ-d 和Ⅱ-e五个亚组。染色体定位表明，存在串联复制和分布不均现象，但是在3号染色体上无该家族基因定位。结构域和保守元件分析都说明葡萄WRKY（VvWRKY） 家族的 WRKY 结构域是高度保守的。葡萄叶片中基因表达模式分析表明， VvWRKY家族的部分基因参与非生物胁迫。
Abstract:
In order to identify and analyze WRKY transcription factor family in grape genome,gene classification,phylogenetic analysis,chromosome location,sequence alignment,conserved motif analysis and expression profiles of VvWRKY proteins were analyzed by bioinformatics method.The results showed that there were 56 WRKY genes in the Vitis vinifera genome.Sequence alignment and phylogenetic analysis showed that they were divided into three main groups(groupⅠ,Ⅱand Ⅲ),with five subgroups(groupⅡ-a,Ⅱ-b,Ⅱ-c,Ⅱ-d and Ⅱ-e) in groupⅡ.The chromosome localization was found that they were localized in all chromosomes except chromosome 3,and these genes were duplicated and distributed in chromosomes.The WRKY domain analysis demonstrated that the WRKY regions were highly conserved.Expression analysis indicated that the expression levels of most VvWRKY genes were altered in response to abiotic stress response.

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备注/Memo

收稿日期：2018-09-13

基金项目：山东省农业重大应用技术创新资助项目（特色酒用葡萄产期精准调控与产品创新研究应用资助项目（2017）、山东葡萄酒地标性酒种开发与关键技术集成应用资助项目（2016））。
第一作者简介：苏玲（1988-），女，博士，农艺师，从事葡萄酒分子生物学研究。E-mail：susulingzi@126.com。通讯作者：陈万钧（1961-），男，学士，高级工程师。从事葡萄栽培研究。E-mail:sdvwac@139.com。

更新日期/Last Update: 2019-03-11