ZHAO Meng-yang,YIN Fa-ting,WANG Jiang-li.Effects of Nitrogen Application Rates of Previous Crop on Soil Microbial Diversity and Urease Activity in Spring Wheat-Silage Maize Mode[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(11):14-19.[doi:10.11942j.issn1002-2767.2018.11.0014]
春小麦-青贮玉米前茬不同施氮量对土壤微生物多样性及脲酶活性的影响
- Title:
- Effects of Nitrogen Application Rates of Previous Crop on Soil Microbial Diversity and Urease Activity in Spring Wheat-Silage Maize Mode
- 文献标志码:
- A
- 摘要:
- 为探究适宜北疆地区滴灌小麦-青贮玉米种植模式下的合理施氮方式，实现作物高产高效。本文通过在滴灌小麦-青贮玉米模式中前茬小麦设置W0（0 kg?hm-2）、W1（300 kg?hm-2）以及W2（30 kg?hm-2）3个不同施氮量处理，研究不同施氮量对后茬根系土壤微生物多样性及脲酶活性的影响。结果表明：前茬施氮处理的微生物多样性均低于未施肥处理，在Phylum、Order、Family与Genus四个分类水平上，各处理的微生物类群OTU数差异性均不显著（P>0.05），具体表现为W0＞W2＞W1，与Simpson、Shannon多样性指数表现一致；Chao1与ACE指数均表现为W2＞W0＞W1；施肥处理的微生物多样性随施氮量增加而上升，即W2＞W1，没有显著性差异（P>0.05）；脲酶活性随前茬施氮量的增加而上升，差异性不显著（P>0.05），施氮处理的脲酶活性与微生物多样性表现相一致。
- Abstract:
- In order to explore the mechanism of yield-increasing effect of the optimal allocation of nitrogen in the drip irrigation wheat-silage corn planting mode,we assessed the effects of different nitrogen application rates on soil microbial diversity and urease activity in the planting mode by setting three nitrogen levels,i.e.W0（0 kg?hm-2）,W1（300 kg?hm-2）,W2（450 kg?hm-2）,in the previous crop.The results showed that microbial diversity in the nitrogen treatments was higher than that in treatment without nitrogen of the preceding crop of wheat-silage corn mode.At the Phylum,Order,Family and Genus levels,the differences in the number of OTUs among microbial groups were not significant(P>0.05),the specific performance for W0>W2>W1.Similar trends were observed in Simpson and Shannon indices.Chao1 and ACE indices were shown as W2>W0>W1.Although the microbial diversity of fertilization treatment increase with the increase of nitrogen fertilization(i.e.,W2>W1),no significant difference(P>0.05) was found.In wheat-silage corn preceding treatments,the urease activity increased with the application of nitrogen fertilizer.Urease activity in nitrogen fertilizer application treatments was consistent with microbial diversity.
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备注/Memo
收稿日期：2018-06-14