LIN Si-si,PU Xiao-qiu,FEI Guang-xue,et al.Study on the Changes of Metabolism Enzyme Activity and Sugar Components in Winter Jujube and Jun Jujube During Fruit Development[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(07):8-11.[doi:10.11942/j.issn1002-2767.2018.07.0008]
冬枣和骏枣果实发育过程中糖组分及代谢酶活性变化的研究
- Title:
-
Study on the Changes of Metabolism Enzyme Activity and Sugar Components in Winter Jujube and Jun Jujube During Fruit Development
- 文献标志码:
- A
- 摘要:
- 为深入研究果实糖积累机制,以冬枣、骏枣果实为试验材料,测定并分析枣果实发育过程中糖组分及其含量以及相关代谢酶活性的变化规律。结果表明:冬枣、骏枣果实均为蔗糖积累型,生长发育早期以积累葡萄糖和果糖为主,后期以快速积累蔗糖为主,且蔗糖含量的积累均表现前期缓慢-后期快速增加趋势,而果糖和葡萄糖含量变化趋势不同;骏枣和冬枣坐果初期果实中蔗糖转化酶活性表现为发育前期急剧下降-后期缓降、蔗糖磷酸合成酶(SPS)活性和蔗糖合成酶(SS)合成方向活性总体升高、SS分解方向活性总体下降的趋势。两个品种果实SS合成方向活性在8月26日前均小于分解方向,但之后则相反。SS分解方向活性则以冬枣始终大于骏枣,SPS活性以骏枣总体大于冬枣;结合骏枣和冬枣果实糖积累与代谢酶活性变化的关系分析,4种酶共同影响枣果实的糖积累,蔗糖积累受SS调控为主,葡萄糖、果糖积累受转化酶调控为主。
- Abstract:
- In order to study the mechanism of fruit sugar accumulation,two processing jujube fruits(winter jujube and Jun jujube) were used to study and analyze the changes of sugar components,sugar contents and enzyme activities involved in related metabolism during fruit development.The results showed that sucrose was accumulated in winter jujube and Jun jujube,during development of the early growth accumulated more fructose and glucose,in the late stage rapid accumulated more sucrose.The accumulations of sucrose contents showed gently decline in early growth and rapidly increase in late stage,but the changes of fructose and glucose contents tendency were different.The early stage of fruit setting of winter jujube fruit and Jun jujube fruit’ activity of invertase was rapidly decline in the early growth and gently decline in the late stage.The totally activity of sucrose phosphate synthetase(SPS) and sucrose synthetase of synthetic direction(SS-s) was increasing,sucrose synthetase of cleavage direction(SS-c) was declining.The two cultivar of fruit activity of SS-s was less than activity of SS-c in August 26th,but on the contrary in the later.The activity of winter jujube SS-c was greater than Jun jujube,the totally activity of Jun jujube SPS was greater than winter jujube.The analyzing was via the accumulations of sugar combined metabolic enzyme activities which of Winter jujube and Jun jujube,four enzyme was common influence the accumulations of fruit sugar.Sucrose accumulated was more impacted SS and fructose and glucose was impacted Invertase.
参考文献/References:
[1]王晨,房经贵,王涛,等.果树果实中的糖代谢[J].浙江农业学报,2009(5):529-530.
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备注/Memo
收稿日期:2018-02-02基金项目:国家级大学生创新创业训练计划资助项目(201610757005)。第一作者简介:林思思(1996-),女,在读学士,从事果树种质资源与品种选育研究。E-mail:1078754465@qq.com。通讯作者:吴翠云(1968-),女,博士,教授,硕导,从事果树种质资源与品种选育研究。E-mail:wcyby@163.com。