[1]刘巾瑞,迟宇新,林俊俊,等.低温处理不同恢复时间高粱幼苗渗透调节相关指标的变化[J].黑龙江农业科学,2018,(07):4-7.[doi:10.11942/j.issn1002-2767.2018.07.0004]
　LIU Jin-rui,CHI Yu-xin,LIN Jun-jun,et al.Variations of Osmotic Adjustment-related Indexes in Different Recovery Time of Sorghum Seedlings Treatedat Low Temperature[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(07):4-7.[doi:10.11942/j.issn1002-2767.2018.07.0004]
点击复制

低温处理不同恢复时间高粱幼苗渗透调节相关指标的变化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年07期 页码: 4-7 栏目: 出版日期: 2018-07-10
Title:
Variations of Osmotic Adjustment-related Indexes in Different Recovery Time of Sorghum Seedlings Treatedat Low Temperature
作者:
刘巾瑞1; 2; 迟宇新1; 林俊俊1; 董洁静1; 朱广石2; 赵长江1; 聂鑫1
1.黑龙江八一农垦大学 农学院/黑龙江省寒地作物现代栽培及种质改良重点实验室/黑龙江省秸秆资源化利用工程技术研究中心，黑龙江 大庆 163319；2.黑龙江省农垦科学院 作物研究所，黑龙江 哈尔滨 150038
Author(s):
LIU Jin-rui1; 2; CHI Yu-xin1; LIN Jun-jun1; DONG Jie-jing1; ZHU Guang-shi2; ZHAO Chang-jiang1; NIE Xin1
1.College of Agriculture,Heilongjiang Bayi Agricultural University,Heilongjiang Provincial Key Laboratory of Modern Crops Cultivation and Germplasm Improvement,Heilongjiang Engineering Technology Research Center for Crop Straw Utilization,Daqing 163319,China; 2.Crops Institute of Heilongjiang Land Reclamation Academy of Sciences,Harbin 150038,China
关键词:
高粱; 低温; 渗透调节
Keywords:
sorghum bicolor;  low temperature;  osmotic adjustment
DOI:
10.11942/j.issn1002-2767.2018.07.0004
文献标志码:
A
摘要:
为揭示低温对高粱幼苗生长的影响，以高粱龙杂17为研究对象，对三叶一心期高粱幼苗进行5 ℃低温处理，分别于恢复1、3、5和7 d取样测定生理生化指标。结果表明：与未处理对照相比，除低温恢复1 d的高粱幼苗根冠比显著抑制外，其余处理均表现为显著增加；高粱幼苗的株高和地上鲜重变化在供试时间点均表现为显著抑制。供试时间点脯氨酸和可溶性糖含量基本都增加，其中恢复1 和3 d的高粱幼苗脯氨酸含量显著增加，恢复3 d的高粱幼苗可溶性糖含量显著增加。高粱幼苗TBARS含量也在整个供试期增加，在恢复3 和5 d显著增加；但过氧化氢含量变化不显著。上述结果表明，低温处理造成了生物膜不同程度的损伤，脯氨酸和可溶性糖等渗透调节在高粱幼苗耐冷的过程中发挥重要作用。
Abstract:
In order to reveal the effects of low temperature on the growth of sorghum seedlings,the three-leaf with one new leaf sorghum Longza 17 seedlings were treated with low temperature at 5 ℃,and they were restored and collected at 1,3,5 and 7 d,respectively.The indicators of growth and physiological and biochemical function were analyzed.The results showed that compared with the untreated controls,except for the root-shoot ratio of sorghum seedlings under 1 d recovered was significant suppression,the other treatments showed a significant increase; the plant height and fresh weight of above ground of sorghum seedlings were significantly inhibited at the time tested.The contents of proline and soluble sugar always increased.Among them,the proline content significantly increased at 1 and 3 d,and the content of soluble sugar significantly increased at 3 d.The contents of TBARS in sorghum seedlings also increased during the whole period tested,and significant increase showed at 3 and 5 d.However,the contents of hydrogen peroxide remained unchanged significantly.The above results indicated that low temperature treatment caused the various degree damage of membranes,and osmotic adjustment materials such as proline,played an important role in tolerance to low temperature of sorghum seedlings.

参考文献/References:

[1]马纯艳,李玥莹,邹建秋,等.低温对高粱后代选系苗期生理生化的影响[J].沈阳农业大学学报,2006,37(2):147-150.

[2]史红梅,张桂香,张海燕,等.低温胁迫对高粱幼苗脯氨酸积累的影响［J］.山西农业科学,2014，42（9）：945-947
[3]张海燕,史红梅,杨彬,等.外源水杨酸对高粱耐冷性的影响[J].天津农业科学,2017,23(5):19-22.
[4]Hodges D M,Delong J M,Forney C F,et al.Improving the thiobarbituric acid-reactive-substance assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds[J].Planta,1999,207:604-611.
[5]Lutts S,Kinet J M,Bouharmont J.NaCl-induced senescence in leaves of rice(Oryza sativa L.) Cultivars differing in salinity resistance[J].Ann Bot,1996,78:389-398.
[6]Velikova V I,Edreva Y A,Oxidative stress and some antioxidant systems in acid rain-treated bean plants-protective role of exogenous polyamines[J].Plant Sci,2000,151:59-66.
[7]Bates L S,Waldren R P,Teare I D.Rapid determination of free proline for water-stress studies[J].Plant Soil,1973,39:205-207.

相似文献/References:

[1]马长山,王胜华,张浩然,等.88%异丙甲草胺乳油防除高粱田杂草田间药效评价[J].黑龙江农业科学,2014,(07):70.
　MA Chang-shan,WANG Sheng-hua,ZHANG Hao ran,et al.Efficacy Evaluation of 88% Metolachlor EC on Sorghum Weed[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):70.
[2]曾宪国,项洪涛,王立志,等.孕穗期不同低温对水稻空壳率的影响[J].黑龙江农业科学,2014,(06):19.
　ZENG Xianguo,XIANG Hongtao,WANG Lizhi,et al.Effect of Different Low Temperature on the Percentage of Rice Unfilled Grains in Booting Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):19.
[3]沈海军.黑龙江省高粱出苗不全的原因与对策[J].黑龙江农业科学,2012,(02):152.
　SHEN Hai-jun.Reasons and Countermeasures of Sorghum Incomplete Emergence in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):152.
[4]王粟,刘杰,裴占江,等.提高低温沼气发酵效果的研究[J].黑龙江农业科学,2012,(05):53.
　WANG Su,LIU Jie,PEI Zhan-jiang,et al.Study on Improving the Effect of Biogas-fermentation at Low Temperature[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):53.
[5]邢慧清,黄赛楠,张云鹤,等.分子标记在高粱抗病虫基因定位上的研究进展[J].黑龙江农业科学,2012,(09):139.
　XING Hui-qing,HUANG Sai-nan,ZHANG Yun-he,et al.Research Advances of Mapping the Sorghum’s Resistance Genes to Disease and Inset by Molecular Markers[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):139.
[6]王春艳,王立志,李锐,等.黑龙江水稻冷害Ⅶ苗期低温对水稻秧苗电导率及可溶性糖含量的影响[J].黑龙江农业科学,2010,(05):21.
　WANG Chun-yan,WANG Li-zhi,LI Rui,et al.Rice Cooling Injury In Heilongjiang ProvinceⅦ Effect of Low Temperature on Relative Electric Conductivity and Soluble Sugar Content of Rice Seedlings[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(07):21.
[7]李鹤鹏,杨广益.高粱除草药剂的筛选[J].黑龙江农业科学,2010,(12):65.
　LI He-peng,YANG Guang-yi.Preemergence and Postemergence Herbicides Screening in Sorghum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(07):65.
[8]边道林,孙磊,王爽,等.嗜冷产甲烷菌群的选育及产甲烷效能研究[J].黑龙江农业科学,2014,(10):44.
　BIAN Dao-lin,SUN Lei,WANG Shuang,et al.Study on Cultivation and Performance of Psychrophilic Methane-Producing Bacteria[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):44.
[9]焦少杰,王黎明,姜艳喜,等.黑龙江省高粱生产发展演变过程分析[J].黑龙江农业科学,2015,(05):138.[doi:10.11942/j.issn1002-2767.2015.05.0138]
　JIAO Shao-jie,WANG Li-ming,JIANG Yan-xi,et al.Analysis on Evolution Process of Sorghum Production Development in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(07):138.[doi:10.11942/j.issn1002-2767.2015.05.0138]
[10]季志强,张 勤,赵共鹏,等.承德地区高粱发展现状与应对措施分析[J].黑龙江农业科学,2015,(06):133.[doi:10.11942/j.issn1002-2767.2015.06.0133]
　JI Zhi-qiang,ZHANG Qin,ZHAO Gong-peng,et al.Current Status and Countermeasures of the Sorghum Production in Chengde Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(07):133.[doi:10.11942/j.issn1002-2767.2015.06.0133]

备注/Memo

收稿日期：2018-03-13基金项目： 中央引导地方科技发展专项资助项目（ZY16A06）; 黑龙江农垦总局科技攻关资助项目（HNK135-02-05）。第一作者简介：刘巾瑞（1993-），女，在读硕士，从事作物逆境生理方向研究。 E-mail：445471830@qq.com。通讯作者：赵长江（1979-），男，博士，副教授，从事作物逆境生理及秸秆炭化利用研究。E-mail：zhaocj15@126.com。

更新日期/Last Update: 2018-09-13