[1]朱宏芬,刘健,黄坚.60Co-γ射线对蓝莓组培苗的诱变效应[J].黑龙江农业科学,2018,(05):21-25.[doi:10.11942/j.issn1002-2767.2018.05.0021]
　ZHU Hong-fen,LIU Jian,HUANG Jian.Effects of 60Co-γ Rays on in vitro Mutagenesis of Blueberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(05):21-25.[doi:10.11942/j.issn1002-2767.2018.05.0021]
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60Co-γ射线对蓝莓组培苗的诱变效应

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年05期 页码: 21-25 栏目: 出版日期: 2018-05-10
Title:
Effects of 60Co-γ Rays on in vitro Mutagenesis of Blueberry
作者:
朱宏芬; 刘健; 黄坚
宁波市农业科学研究院,浙江 宁波 315040
Author(s):
ZHU Hong-fen;  LIU Jian; HUANG Jian
Ningbo Academy of Agricultural Sciences，Ningbo 315040,China
关键词:
蓝莓; 60Co-γ; 诱变效应
Keywords:
blueberry; 60Co-γ; mutagenic effect
DOI:
10.11942/j.issn1002-2767.2018.05.0021
文献标志码:
A
摘要:
为探讨不同剂量60Co-γ射线对蓝莓组培苗的诱变效应，以奥尼尔、南大、南金、莱格西蓝莓无菌组培苗为试材，采用6个剂量的60Co-γ射线辐射处理，研究辐照组培苗继代和移栽的存活率以及生长变异情况。结果表明：随着辐射剂量的增加，组培继代苗的存活率、增殖率、苗高都呈下降趋势，高剂量辐射使叶片变小，叶色变淡，超过80 Gy后生长停滞，逐渐褐化死亡。辐照对蓝莓组培移栽苗的存活率和长势均有显著影响，存活率、苗高和基生枝粗随着剂量增加而显著下降，辐照各处理对继代组培苗移栽存活率影响较小。4个品种组培苗继代生长的半致死剂量分别为 79.4、68.2、81.7和72.2 Gy；组培辐照苗直接移栽的半致死剂量分别为 69.2、57.5、75.6和67.6 Gy。
Abstract:
In order to investigate the mutagenic effect of different doses of 60Co-gamma ray on blueberry tissue culture seedlings,four blueberry cultivars(O’Neal，Southbig,Southgold,and Legacy)in vitro were irradiated by 60Co-γrays from 20 to 120 Gy for induced mutation breeding,to study on the survival rate and growth variation irradiated tissue culture seedings．The results indicated that with the increase of radiation dose,the survival rate,proliferation rate and the height of the tissue culture plants all declined.The high dose radiation made the leaves smaller and the leaf color were light,growth stagnated and died exceed 80 Gy.The survival rate and the growth potential of the transplanted blueberry plants were significantly affected by irradiation and decreased significantly with the increase of dose.However，the effects of transplanting survival rate of blueberry tissue subculture plants were less.the LD50 of four varieties (O’Neal，Southbig,Southgold,Legacy) in proliferation culture were 79.4，68.2，81.7 and 72.2 Gy，and in transplanting were 69.2,57.5,75.6 and 67.6 Gy.

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备注/Memo

收稿日期：2018-01-08基金项目：宁波市科技局农业科技重大专项资助项目(2014C11004)。第一作者简介：朱宏芬（1974-），女，学士，高级农艺师，从事蓝莓育种、栽培技术研究。E-mail：nbnkyzhu@163.com。

更新日期/Last Update: 2018-08-02