[1]王丽娜,金勋,李泽宇,等.一种高效的蝴蝶兰花梗诱导丛生芽体系[J].黑龙江农业科学,2018,(05):17-20.[doi:10.11942/j.issn1002-2767.2018.05.0017]
　WANG Li-na,JIN Xun,LI Ze-yu,et al.An Efficient System for Inducing Cluster Buds of Phalaenopsis Pedicels[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(05):17-20.[doi:10.11942/j.issn1002-2767.2018.05.0017]
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一种高效的蝴蝶兰花梗诱导丛生芽体系

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年05期 页码: 17-20 栏目: 出版日期: 2018-05-10
Title:
An Efficient System for Inducing Cluster Buds of Phalaenopsis Pedicels
作者:
王丽娜; 金勋; 李泽宇; 顾鑫; 齐国超
黑龙江省农业科学院 大庆分院，黑龙江 大庆 163316
Author(s):
WANG Li-na; JIN Xun; LI Ze-yu; GU Xin; QI Guo-chao
Daqing Branch，Heilongjiang Academy of Agricultural Sciences,Daqing 163316,China
关键词:
花梗腋芽; 诱导; 增殖和分化; 蝴蝶兰
Keywords:
pedicel axillary buds;  induction;  proliferation and differentiation;  Phalaenopsis amabilis
DOI:
10.11942/j.issn1002-2767.2018.05.0017
文献标志码:
A
摘要:
为提高蝴蝶兰组织培养效率，分别以幼嫩花梗和已开花蝴蝶兰花梗腋芽为外植体，采用两种消毒剂HgCl2、NaClO，3种消毒方式0.1%HgCl2，10 min ；10%NaClO，10 min；5%NaClO，10 min为处理，以VW（大量）+MS（微量、铁盐、有机）+100 g?L-1马铃薯+0.1 g?L-1Ga3（PO4）2+6 mg?L-16-BA+0.2 mg?L-1NAA为诱导基本培养基；VW（大量）+MS（铁盐、有机、微量）+100 g?L-1马铃薯+ 3 mg?L-16-BA +0.2 mg?L-1NAA为增殖分化培养基；1/2MS+0.1 mg?L-1NAA为生根培养基诱导丛生芽，拟建立以蝴蝶兰花梗腋芽为外植体的高效丛生芽诱导体系。结果表明：在0.1%HgCl2，10 min消毒方式下，幼嫩花梗腋芽的污染率为0，丛生芽诱导率为95.6%；增殖率为216%；在生根培养基1/2MS+ 0.1 mg?L-1NAA上生根率为95.6%，建立了高效的蝴蝶兰幼嫩花梗腋芽诱导丛生芽。
Abstract:
In order to improve the efficiency of tissue culture of Phalaenopsis,we used the axillary buds of young and flowering butterfly orchids as explants,and adopted two disinfectants(HgCl2 and NaClO),and three disinfection methods(0.1%HgCl2,10 min；10%NaClO,10 min;5% NaClO，10 min) to compare the disinfection effects.The mediums were as follows,VW (A large number of elements) +MS (trace,iron,organic)+100 g? L-1 potato+0.1 g ?L-1Ga3 (PO4) 2+6 mg?L-16-BA+0.2 mg ?L-1 NAA as the basic medium,VW (A large number of elements) +MS (trace,iron,and organic) + 100 g?L-1potato + 3 mg?L-16-BA+ 0.2 mg?L-1NAA as proliferation and differentiation medium,and 1/2MS +0.1 mg? L-1NAA induced clustered buds for rooting medium.The results showed that the induction rates of pollution rate was 0,the young shoots of axillary buds were 95.6%,the reproducibility rate was 216%,and the rooting rate of 1/2MS+0.1 mg?L-1NAA in the rooting medium was 95.6%,under 0.1% HgCl2 10 min disinfection method indicated the use of two kinds of medium,disinfection methods and induced proliferation and differentiation,was suitable for inducing shoots of axillary buds of young peduncle.

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备注/Memo

收稿日期：2018-03-01基金项目：大庆市指导性科技计划资助项目（zd-2016-118）。第一作者简介：王丽娜（1983-），女，硕士，助理研究员，从事植物组织培养及生理生化研究。E-mail：dqnkywln@163.com。通讯作者：金勋（1962-），男，博士，研究员，从事作物分子育种研究。E-mail：jinxun6268@126.com。

更新日期/Last Update: 2018-08-02