[1]郝瑞芳,于蓉,张先平,等.葡萄原花青素和花青素对丙烯酰胺致细胞损伤的保护作用[J].黑龙江农业科学,2018,(04):122-127.[doi:10.11942/j.issn1002-2767.2018.04.0122]
　HAO Rui-fang,YU Rong,ZHANG Xian-ping,et al.Protective Effects of Grape Procyanidine and Anthocyanin on Cell Injuries Induced by Acrylamide[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(04):122-127.[doi:10.11942/j.issn1002-2767.2018.04.0122]
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葡萄原花青素和花青素对丙烯酰胺致细胞损伤的保护作用

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年04期 页码: 122-127 栏目: 出版日期: 2018-04-10
Title:
Protective Effects of Grape Procyanidine and Anthocyanin on Cell Injuries Induced by Acrylamide
作者:
郝瑞芳; 于蓉; 张先平; 侯艳霞; 张兴亮
山西林业职业技术学院 园艺系，山西 太原 030009
Author(s):
HAO Rui-fang; YU Rong; ZHANG Xian-ping; HOU Yan-xia; ZHANG Xing-liang
Department of Horticulture,Shanxi Forestry Vocational Institute,Taiyuan 030009,China
关键词:
葡萄籽原花青素提取物; 葡萄皮花青素提取物; 丙烯酰胺; 细胞损伤
Keywords:
grape seed procyanidine extract (GSPE);  grape hull anthocyanin extract (GHAE);  acrylamide;  cells injury
DOI:
10.11942/j.issn1002-2767.2018.04.0122
文献标志码:
A
摘要:
用丙烯酰胺诱导人结肠癌细胞HT-29和人神经母细胞瘤SH-SY5Y损伤建立细胞损伤模型，研究葡萄籽原花青素和花青素提取物对两种细胞的保护作用。结果表明：用100～700 μg?mL-1丙烯酰胺分别对HT-29和SH-SY5Y细胞孵育6和8 h，两种细胞存活率分别下降至44.6%～80.5%和52.0%～81.0%，表明丙烯酰胺对细胞均有损伤作用。经葡萄籽提取物或葡萄皮提取物（5、10、20 μg?mL-1）预先对HT-29细胞孵育1 h后，再用300 μg?mL-1丙烯酰胺处理6 h，HT-29细胞的存活率均显著高于丙烯酰胺损伤组（69.8%），其中10 μg?mL-1的葡萄籽提取物和葡萄皮提取物对HT-29细胞的保护作用最强，细胞存活率分别达到92.4%和93.8%。用葡萄皮提取物（5、10、20 μg?mL-1）和葡萄籽提取物（5 μg?mL-1）对SH-SY5Y细胞预先孵育1 h，同样能够显著增加SH-SY5Y细胞的存活率。其中，葡萄皮提取物的保护作用较强，低、中、高浓度组的细胞存活率分别为80.7%、89.3%、91.5%。葡萄原花青素和花青素具有抑制细胞损伤的作用。
Abstract:
The preventive effects of grape seed procyanidine extract (GSPE) and grape hull anthocyanin extract (GHAE) on acrylamide-induced cell injuries of human colon cancer cells (HT-29) and human neuroblastoma cells (SH-SY5Y) were investigated by using cellular morphology in this paper.The results showed that the cell viabilities were decreased to 44.6%-80.5% when HT-29 cells were incuvated 6 h with acrylamide of 100-700 μg?mL-1.The cell viabilities were decreased to 52.0%-81.0% when SH-SY5Y cells were incuvated 8 h with acrylamide of 100-700 μg?mL-1,and it showed that acrylamide induced these two cells damage.However,when HT-29 cells were pre-protected 1 h with GSPE or GHAE of 5,10 and 20 μg?mL-1 before incubation 6 h with acrylamide of 300 μg?mL-1,GSPE and GHAE resulted in significant increases of cell viability respectively,as compared with that in acrylamide injury group (69.8%).GSPE and GHAE had the maximum protetive effects on HT-29 cell from AA-induced cytotoxicity,with HT-29 cell viability of 92.4% and 93.8%.Moreover,compared with acrylaimide group,pretreatment of GHAE (5,10 and 20 μg?mL-1) and GSPE (5 μg?mL-1) resulted in significant increases of cell viabilities on SH-SY5Y cell.GHAE had better protetive effects on SH-SY5Y cell than GSPE,with the cell viabilities of 80.7%,89.3% and 91.5% at 5,10 and 20 μg?mL-1.GSPE and GHAE could effectively protect HT-29 cells and SH-SY5Y cells from acrylamide-induced injury.

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备注/Memo

收稿日期：2018-01-07基金项目：山西省科技厅青年基金资助项目（2015021154）；山西林业职业技术学院科研教改基金资助项目（20170207）。第一作者简介：郝瑞芳（1978-），女，博士，副教授，从事食品安全与质量控制研究。E-mail: 471518969@qq.com。

更新日期/Last Update: 2018-05-17