[1]张誉稳,李孟南,赵雁.月季定品种波塞尼娜变异株的发现与初步认[J].黑龙江农业科学,2018,(04):85-88.[doi:10.11942/j.issn1002-2767.2018.04.0085]
　ZHANG Yu-wen,LI Meng-nan,ZHAO Yan.The Primary Cognizance of New Variation of Rosa hybrida L.Porcelina[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(04):85-88.[doi:10.11942/j.issn1002-2767.2018.04.0085]
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月季定品种波塞尼娜变异株的发现与初步认

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年04期 页码: 85-88 栏目: 出版日期: 2018-04-10
Title:
The Primary Cognizance of New Variation of Rosa hybrida L.Porcelina
作者:
张誉稳; 李孟南; 赵雁
云南农业大学 园林园艺学院，云南 昆明 650201
Author(s):
ZHANG Yu-wen; LI Meng-nan; ZHAO Yan
College of Horticulture and Landscape，Yunnan Agricultural University，Kunming 650201，China
关键词:
波塞尼娜; 蔷薇属DUS测试; 倍性鉴定
Keywords:
Rosa hybrida L.Porcelina; DUS guidelines of Rosa;  ploidy identification
DOI:
10.11942/j.issn1002-2767.2018.04.0085
文献标志码:
A
摘要:
为探究月季品种波塞尼娜繁育过程中发现的优株是否具有新品种特性，依据蔷薇属DUS测试指南对其外部形态进行测定，并通过叶片气孔长度和流式细胞仪测试结果推断其倍性。结果表明：疑似变异株的花朵直径、花瓣数量、花瓣长度和宽度、叶片长度和宽度、茎秆上下部粗细、株高分别高于正常株34.77%、18.87%、28.43%、38.20%、47.72%、52.24%、50.92%、62.74%、32.42%；叶片气孔数量高于正常株6.93%，气孔长度低于正常植株0.09%；流式细胞术结果显示疑似变异株未发生整倍体变异，两者倍性趋于一致，均为四倍体；通过与已知月季变异品种比较，初步推断波塞尼娜疑似变异株是一个芽变株系。
Abstract:
In order to explore whether the new variation of Rosa hybrida L.Porcelina which we discovered in productive process have new plants characteristics.We measured its external morphology,according to DUS guidelines of rosa,and inferred its ploidy by stomatal length and Flow cytometer.The results showed that the suspected variation plant were higher 34.77%,18.87%,28.43%,38.20%,47.72%,52.24%,50.92%,62.74% and 32.43% than the normal plant in flowers diameter,petals number,petals length and width,leaves length and width,upper stem diameter,under stem diameter and plant height.The variation plant′s stomatal number in leaves was higher than normal plant for 6.9% and stomatal length was lower than normal plant for 0.09%,and ploidy of them tend to be same.The results of Flow cytometer showed that there were no aneuploidy variation in variation rose,and the two tended to be consistent,both of which were tetraploid.It can preliminarily inferred that suspected variation plant is a sport lines by comparing with known rose varieties.

参考文献/References:

[1]许凤,张颢,杨春梅,等.利用隶属函数值法评价月季耐寒性[J].西南农业学报,2012,25(5): 1870-1873.
[2]吴昊,任永波,余前媛.保鲜剂对切花月季的生理效应研究[J].西南农业学报,2007(5): 1088-1090.
[3]孟令宁,晏慧君,张颢,等.大花香水月季再生体系的初步建立[J].云南农业大学学报,2012,27(6): 870-874.
[4]王国良,上田善弘,巫水钦.切花月季芽变品种的分子标记与鉴别研究[J].江苏林业科技,2001(1): 1-9.
[5]孙曾丽,刘耀玺,王天旭.‘锦上添花’月季选育研究[J].林业科技通讯,2016(9): 53-55.
[6]谢吉容,梁国鲁,李树发,等.月季 ‘金银岛’的红花芽变品系的分析鉴定[J].北方园艺,2007(11): 186-188.
[7]宗荣林.月季芽变育种[J].中国花卉盆景,1996(4):5.
[8]秦贺兰.观赏植物芽变育种研究进展[J].北京园林,2009,25(3):33-36.
[9]常牛山,樊花妍,党建军.大花月季却可克芽变前后形态特征观察[J].特种经济动植物,2016(10):37-38.
[10]张军云,张钟,董春富,等.月季新品种‘温馨’的选育[J].北方园艺,2017(8):164-165.
[11]岳玲.月季新品种‘红坤特利’[J].北方园艺,2014(2):82.
[12]黄平.月季品种分子鉴定与遗传关系分析[D].北京：中国林业科学研究院,2012.
[13]宋金耀,刘永军,王贺山,等.山海关杨一变异株的发现和初步认证[J].华北农学报,2008(S1): 297-300.
[14]杨艳琼,何丽萍,和凤美,等.用气孔保卫细胞叶绿体计数法鉴定烟草染色体倍性方法初探[J].种子,2002(3): 24-25，56.
[15]武荣花,葛蓓蓓,王茂良,等.应用流式细胞术测定18个中国古老月季基因组大小[J].北京林业大学学报,2016(6): 94-100.
[16]中华人民共和国林业行业标准LY/T 1868-2012-植物新品种特异性、一致性、稳定性测试指南-蔷薇属[Z].2010-02-09.
[17]Joly S,Bruneau A.Delimiting species boundaries in Rosa sect.Cinnamomeae(Rosaceae) in eastern North America[J].Systematic Botany,2007,32(4): 819-836.
[18]Roberts A V,Lloyd D,Short K C.In vitro,procedures for the induction of tetraploidy in a diploid rose[J].Euphytica,1990,49(1): 33-38.
[19]巢阳,周燕.通过测量叶片气孔鉴定月季染色体倍性的研究[J].北京农学院学报,2015(3): 79-85.
[20]丁晓六,刘佳,赵红霞,等.现代月季和玫瑰杂交后代的鉴定与评价[J].北京林业大学学报,2014(5): 123-130.
[21]Giday H,Fanourakis D,Kjaer K H,et al.Foliar abscisic acid content underlies genotypic variation in stomatal responsiveness after growth at high relative air humidity[J].Annals of Botany,2013,112(9): 1857-1867.
[22]Zlesak D C.Pollen diameter and guard cell length as predictors of ploidy in diverse rose cultivars,species,and breeding lines[J].Floriculture and Ornamental Biotechnology,2009.
[23]邹平洲,孙曾丽,赵磊.‘粉扇’月季适应性调查[J].河南林业科技,2014(3): 22-24.
[24]王丽花,段金辉,薛丽萍,等.月季新品种‘金辉’[J].园艺学报,2015(6): 1217-1218.

备注/Memo

收稿日期：2018-01-13第一作者简介：张誉稳（1994-），女，在读硕士，从事园林植物资源利用与创新的研究。E-mail：775197562@qq.com。通讯作者：赵雁（1974-），女，博士，副教授，从事园林植物资源利用与创新研究。E-mail：zhaoyan@ynau.edu.cn。

更新日期/Last Update: 2018-05-17