ZHANG Qian,LI Ming-zhang,WANG Li-hua,et al.Establishment of Regeneration System from Stemwith Axillary Bud of Hongyang Kiwifruit[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(04):5-9.[doi:10.11942/j.issn1002-2767.2018.04.0005]
红阳猕猴桃带芽茎段再生体系的建立
- Title:
- Establishment of Regeneration System from Stemwith Axillary Bud of Hongyang Kiwifruit
- 文献标志码:
- A
- 摘要:
- 为建立红阳猕猴桃快速繁育高效再生体系,以带芽茎段为外植体材料,通过诱导带芽茎段直接出芽,研究不同浓度激素的MS培养基对腋芽萌发、不定芽增殖及生根的影响。结果表明:最适宜带芽茎段腋芽萌发的培养基是MS+1.0 mg?L-1 6-BA+0.1 mg?L-1 NAA,萌芽率达88.37%。在MS+1 mg?L-1 ZT+0.1 mg?L-1 NAA培养基上,不定芽增殖效果最好。1/2MS+0.8 mg?L-1 IBA培养基最利于不定芽诱导生根,根系发达,再生苗长势健壮。
- Abstract:
- In order to establish the rapid breeding and efficient regeneration system of Hongyang Kiwifruit,the stem with axillary bud was used as explants to be cultured in the MS medium with different concentrations of hormones.The effects of different concentrations of hormones on axillary bud sprouting,multiplication and rooting were examined in this study.The results showed that the optimum medium for axillary bud sprouting was MS+1.0 mg?L-16-BA+0.1 mg?L-1NAA,the germination rate was 88.37%.The medium with MS+1 mg?L-1ZT+0.1 mg?L-1NAA had best effect on adventitious bud multiplication.The rooting effect was best on the rooting medium with 1/2 MS+0.8 mg?L-1IBA,roots developed well and plants growed vigorous.
参考文献/References:
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备注/Memo
收稿日期:2017-11-13基金项目:四川省科技厅国合资助项目(2015HH0038);四川省“十三五”农作物及畜禽育种攻关资助项目(2016NYZ0034);四川省中央引导地方科技发展专项资金资助项目(2016TZYD0001);四川省科技支撑计划资助项目(2016NZ0105)。第一作者简介:张茜(1990-),女,硕士,研究实习员,从事猕猴桃资源保存与育种研究。E-mail:zqxabc@126.com。通讯作者:王丽华(1978-),女,硕士,副研究员,从事猕猴桃资源保存与育种研究。E-mail:514935456@qq.com。