[1]刘晓丹,王霞,杨祥波.非生物胁迫下山腊梅CpCBF基因的表达模式分析[J].黑龙江农业科学,2017,(12):18-21.[doi:10.11942/j.issn1002-2767.2017.12.0018]
　LIU Xiao-dan,WANG Xia,YANG Xiang-bo.Expression Profiling Analysis of ?CpCBF ?Gene from Chimonanthus praecox Under Abiotic Stress[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(12):18-21.[doi:10.11942/j.issn1002-2767.2017.12.0018]
点击复制

非生物胁迫下山腊梅CpCBF基因的表达模式分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年12期 页码: 18-21 栏目: 出版日期: 2017-12-10
Title:
Expression Profiling Analysis of ?CpCBF ?Gene from Chimonanthus praecox Under Abiotic Stress
文章编号:
1002-2767（2017）12-0018-03
作者:
刘晓丹; 王霞; 杨祥波
吉林农业科技学院 生物工程学院，吉林 吉林 132101
Author(s):
LIU Xiao-dan; WANG Xia; YANG Xiang-bo
School of Biological Engineering,Jilin Agricultural Science and Technology University,Jilin,Jilin 132101
关键词:
山腊梅; CpCBF基因; 非生物胁迫; 表达模式
分类号:
S945.78
DOI:
10.11942/j.issn1002-2767.2017.12.0018
文献标志码:
A
摘要:
为探讨腊梅CpCBF基因在非生物胁迫下的响应机制，对山腊梅幼苗分别进行低温、干旱、NaCl和ABA 处理，利用 qRT-PCR技术对CpCBF 基因的表达进行检测。结果表明: 腊梅CpCBF基因能快速响应低温、盐、干旱和 ABA 胁迫，其中对低温和干旱的响应较为显著和持久。4 ℃低温处理12 h后，其表达量达峰值；对于干旱胁迫，处理6 h后表达量最大。初步推测CpCBF基因可能在腊梅抵御低温、干旱和高盐等非生物胁迫时发挥重要作用。
Abstract:
In order to explore the mechanism of CpCBF gene from Chimonanthus praecox in response to abiotic stress,the seedlings were treated with low temperature,drought,NaCl and ABA,respectively,the expression of CpCBF?gene were detected using quantitative real-time PCR.The results showed that CpCBF?gene was induced immidately in response to low temperature,drought,salt and ABA,among them,the response to low temperature and drought was more significant and lasting.At 4 ℃ for 12 h,the expression reached its peak.For drought stress treatment,the expression level reached the maximum after 6 h.It is preliminarily speculated that CpCBF gene may play an important role in resistance to abiotic stress such as low temperature,drought and salt stress.Keywords：Chimonanthus praecox; CpCBF gene;abiotic stress;expression profiling

参考文献/References:

[1]李勇鹏,张力维,张佳佳,等.香樟CcCBFs基因的克隆及表达模式[J].东北林业大学学报,2016,44(8):34-40.
[2]李科友,朱海兰.植物非生物逆境胁迫DREB/CBF转录因子的研究进展[J].林业科学,2011,47(1):124-134.
[3]Pagter M,Arora R.Winter survival and deacclimation of perennials under warming climate: physiological perspectives[J].Physiologia Plantarum,2013,147(1): 75-87.
[4]李月,代培红,刘超,等.海岛棉5个CBF/DREB基因的克隆与表达分析[J].棉花学报,2016,28(1):42-51.
[5]Agarwal P K,Agarwal P,Reddy M K,et al.Role of DREB transcription factors in abiotic and biotic stress tolerance in plants[J].Plant Cell Rep,2006,25(12):1263-1274.[6]Shi H，Qian Y，Tan D，et al． Melatonin induces the transcripts of CBF/DREB1s and their involvement in both abiotic and biotic stresses in Arabidopsis[J]．J Pineal Res,2015,59(3):334-342．
[7]Dubouzet J G,Sakuma Y,Ito Y,et al.OsDREB?genes in rice,Oryza sativa L.,encode transcription activators that function in drought-,high salt- and cold-responsive gene expression[J].Plant Journal,2003,33:751-763.
[8]Egawa C,Kobayashi F,Ishibashi M,et al.Differential regulation of transcript accumulation and alternative splicing of a DREB2 homolog under abiotic stress conditions in common wheat[J].Genes Genet Syst，2006，81(2): 77-91．
[9]Li X,Tian A,Luo G,et al.Soybean DRE-binding transcription factors that are responsive to abiotic stresses[J].Theor Appl Genet,2005,110(8): 1355-1362．
[10]Gu L,Zhang Y,Zhang M,et al.ZmGOLS2,a target of transcription factor ZmDREB2A,offers similar protection against abiotic stress as ZmDREB2A[J].Plant Mol Biol,2016,90(1-2): 157-170．
[11]贾会霞,李建波,孙佩,等.胡杨CBF基因家族的鉴定及表达特性分析[J].分子植物育种,2017,15 (2) :492-500.
[12]严君,孙焕,王雪华,等.麻疯树JcCBF3基因的克隆及其抗寒功能初探[J].四川大学学报（自然科学版）,2016,53(6):1369-1374.
[13]郭淑朋,张亮,李疆,等.扁桃AcCBF2基因的克隆及其在逆境胁迫下的表达分析[J].分子植物育种,2017,15(3):814-820.
[14]雷恒久,苏淑钗,马履一,等.平欧杂交榛CBF/DREB1转录因子ChaCBF1基因的克隆与功能分析[J].北京林业大学学报,2016,38(10):69-79.
[15]Jaglo K R,Kleff S,Amundsen K L,et al.Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species[J].Plant Physiol,2001,127(3): 910 -917.
[16]Liu D F,Sui S Z,Ma J,et al.Transcriptomic Analysis of flower development in wintersweet(Chimonanthus praecox）[J].PLoS One,2014,9(1):e86976.
[17]Wang Z,Wang F,Tang J,et al.C-repeat binding factor gene family identified in non-heading Chinese cabbage is functional in abiotic and biotic stress response but different from that in Arabidopsis[J].Acta Physiologiae Plantarum,2014,36(12): 3217-3229.
[18]YangW,Liu X D,Chi X J,et al.Dwarf apple MbDREB1 enhances plant tolerance to low temperature,drought,and salt stress via both ABA-dependent and ABA-independent pathways[J].Planta,2011,233(2):219-229

备注/Memo

收稿日期：2017-10-24

基金项目：吉林省教育厅“十二五”科学技术研究资助项目（吉教科合字[2015]第372号）
第一作者简介： 刘晓丹(1981-)， 女，吉林省吉林市人，博士，讲师，从事抗逆基因工程研究。E-mail: liuxiaodan1981@ 126.com。

更新日期/Last Update: 2018-02-22