MAO Liang,WU Jiu-yun,JI Yan-ling,et al.Determination of Extracellular Enzyme Activity of Hami Melon Bacterial Fruit Blotch[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(11):31-33.[doi:10.11942/j.issn1002-2767.2017.11.0030]
哈密瓜细菌性果斑病胞外酶活性测定
- Title:
- Determination of Extracellular Enzyme Activity of Hami Melon Bacterial Fruit Blotch
- 文章编号:
- 1002-2767（2017）11-0030-03
- Keywords:
- Hami melon; bacterial fruit blotch; enzyme activity; pathogenicity
- 分类号:
- S652.1；S432.4 +2
- 文献标志码:
- A
- 摘要:
- 为了防治哈密瓜细菌性果斑病初期侵染，通过测定哈密瓜细菌性果斑病病菌生长曲线、病菌胞外酶、果胶酶和纤维素酶变化，掌握哈密瓜细菌性果斑病侵染初期胞外酶、果胶酶和纤维素酶活性的变化规律。结果表明:哈密瓜细菌性果斑病病菌同时产生果胶酶和纤维素酶，说明该病菌在侵染初期的致病力跟这两个酶活性有关。结合病菌生长曲线，果胶酶未呈现明显趋势，而纤维素酶随细菌的生长进入衰退期后活性呈逐渐增加趋势，在44 h果胶酶和纤维素酶同时出现波峰，但后期44~72 h果胶酶再没有出现大幅增长趋势，而纤维素酶呈明显上升趋势，说明病害后期的扩展纤维素酶起到了主要作用。
- Abstract:
- In order to prevent the initial Hami melon bacterial fruit spot infection， through the determination of pathogen bacteria growth curve of melon bacterial fruit blotch,extracellular enzymes pectinase change,extracellular cellulase change,the law of extracellular enzyme pectinase and cellulose was mastered in the early stages of infection of melon bacterial fruit blotch.The results showed that pectinase and cellulase were produced by bacterial fruit blotch of melon,which indicated that pathogenicity of the pathogen was related to the activities of these two enzymes at the initial stage of infection.With the growth curve of bacteria,the pectinase did not show obvious trend,but the activity of cellulase increased gradually after the growth of bacteria entered the recession period.Both pectinase and cellulase appeared peaks at the same time in 44 h,but there was no significant increase in pectinase in the late 44 h to 72 h,cellulase showed an obvious upward trend,indicating that cellulase plays a major role in the late growth of the disease.
参考文献/References:
相似文献/References:
[1]苏拉依曼?沙特尔.春提早日光温室小高垄滴灌哈密瓜定植及管理技术要点[J].黑龙江农业科学,2021,(03):137.[doi:10.11942/j.issn1002-2767.2021.03.0137]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(11):137.[doi:10.11942/j.issn1002-2767.2021.03.0137]
备注/Memo
收稿日期：2017-09-10基金项目：新疆维吾尔自治区科技厅基层青年科技人才资助项目（qn2015jc073）；国家自然科学基金资助项目（31460485）第一作者简介：毛亮（1981-），男，山东省即墨市人，硕士，高级农艺师，从事设施病虫害研究。E-mail：mao2548@163.com。通讯作者：王晓东（1975-），男，山东省莱州市人，博士，副教授，从事植物病害生物防治研究。E-mail：wxd_agr@shzu.edu.cn。