[1]王继洋,王坤芃,敬文宪,等.鸭源鸡杆菌外膜蛋白W基因扩增及序列分析[J].黑龙江农业科学,2017,(05):6-11.[doi:10.11942/j.issn1002-2767.2017.05.0006]
　WANG Ji-yang,WANG Kun-peng,JING Wen-xian,et al.Amplification and Sequence Analysis of Outer Membrane Protein W Gene of ?Gallibacterium anatis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(05):6-11.[doi:10.11942/j.issn1002-2767.2017.05.0006]
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鸭源鸡杆菌外膜蛋白W基因扩增及序列分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年05期 页码: 6-11 栏目: 出版日期: 2017-05-10
Title:
Amplification and Sequence Analysis of Outer Membrane Protein W Gene of ?Gallibacterium anatis
文章编号:
1002-2767（2017）05-0006-06
作者:
王继洋; 王坤芃; 敬文宪; 王 艳; 彭志锋; 杨 霞
河南农业大学 禽病研究所，河南 郑州 450002
Author(s):
WANG Ji-yang; WANG Kun-peng; JING Wen-xian; WANG Yan; PENG Zhi-feng; YANG Xia
Institute of Poultry Diseases,Henan Agricultural University,Zhengzhou,Henan 450002
关键词:
鸭源鸡杆菌; ompW; 扩增; 序列分析
Keywords:
Gallibacterium anatis;  ompW gene;  amplification; sequence analysis
分类号:
S852.1
DOI:
10.11942/j.issn1002-2767.2017.05.0006
文献标志码:
A
摘要:
为了解不同鸭源鸡杆菌菌株外膜蛋白W基因（ompW）的差异性，对不同地域32株鸭源鸡杆菌ompW基因进行扩增、测序，并应用DNAstar/MegAlign和MEGA.5.05软件对其进行比对及遗传进化分析。结果表明：ompW基因存在于所有检测的鸭源鸡杆菌中。全长在630~711 bp，编码209~236个氨基酸。与参考株UMN179的ompW核苷酸同源性为88.7%~100%，氨基酸同源性为86.7%~99.6%。不同来源的鸭源鸡杆菌的核苷酸同源率为84.1%~100%，氨基酸同源性为78.7%~99.6%。进化树分析表明，鸭源鸡杆菌ompW序列差异与分离地理位置，毒力无明显的相关性。
Abstract:
To study the distribution of discrepancy of the outer membrane protein W gene (ompW) bacteria in ?Gallibacterium anatis,a pair of specific primers were designed for the?ompW?gene of UMN179,which was published by NCBI.Amplification and sequencing of ompW?gene of 32 strains of G.anatis?from different region were compared using DNAstar/MegAlign and MEGA.5.05 software,as well as the sequence of ompW?gene and the genetic evolution analysis.The results showed that the ompW?gene was detected in G.anatis.Length between 630 ~711 bp,encoding 209~236 amino acids.The ompW?nucleotide identity of the reference strain by 88.7%~100% was UMN179,the homology of amino acid was 86.7%~99.6%.The nucleotide homology of the different source of the G.anatis?was 84.1%~100%,and the amino acid homology was 78.7%~99.6%.Phylogenetic tree analysis,there was no significant correlation between the ompW?sequence and the geographical location of the isolate of the G.anatis.In order to further study its biological function,the establishment of differential diagnosis method and the development of subunit vaccine were provided.

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备注/Memo

收稿日期：2017-03-14基金项目：河南省自然科学基金资助项目（162300410153）；河南省高校科技创新团队与支持计划资助项目（14IRTSHN015）第一作者简介：王继洋（1990-），男，河南省商丘市人，在读硕士，从事动物传染病发病机理及防制研究。E-mail:247132049@qq.com。〖ZK）〗通讯作者：杨霞（1973-），女，博士，副教授，从事动物传染病发病机理及防制研究。E-mail: yangxia66@163.com。

更新日期/Last Update: 2017-08-07