[1]李 宁,张 巍,张治成.己烷雌酚抗原合成和多克隆抗体制备[J].黑龙江农业科学,2016,(10):83-86.[doi:10.11942/j.issn1002-2767.2016.10.0083]
　LI Ning,ZHANG Wei,ZHANG Zhi-cheng.Synthesis of Antigen and Preparation of Multi-colonial Antibody Against Hexoestrol[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(10):83-86.[doi:10.11942/j.issn1002-2767.2016.10.0083]
点击复制

己烷雌酚抗原合成和多克隆抗体制备

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2016年10 页码: 83-86 栏目: 出版日期: 2016-10-10
Title:
Synthesis of Antigen and Preparation of Multi-colonial Antibody Against Hexoestrol
文章编号:
1002-2767（2016）10-0083-04
作者:
李 宁; 张 巍; 张治成
（哈尔滨市兽药饲料监察所，黑龙江 哈尔滨 150070）
Author(s):
LI Ning; ZHANG Wei; ZHANG Zhi-cheng
(Institute of Veterinary Drug and Food Control in Harbin,Harbin,Heilongjiang 150070）
关键词:
己烷雌酚; 多克隆抗体; 酶联免疫吸附分析
Keywords:
hexoestrol;  multi-clonial antibody;  ELISA
分类号:
S859.84
DOI:
10.11942/j.issn1002-2767.2016.10.0083
文献标志码:
A
摘要:
为了对己烷雌酚进行检测，采用混合酸酐法，合成具有己烷雌酚基本分子结构特性的半抗原。以己烷雌酚-牛血清白蛋白为抗原免疫家兔，获得了特异性的己烷雌酚多克隆抗体。结果表明：己烷雌酚半抗原与牛血清白蛋白偶联的摩尔比为12.93∶1。今后可将所得抗体用于间接竞争性酶联免疫吸附分析法用于己烷雌酚的检测。
Abstract:
In order to detect hexoestrol,the antibody of HES was prepared with anhydride method for detecting HES residues later.Hapten with carboxylic that preserved the molecule structure character of diethylstilbestrol was synthesized.Polyclonal antibodies for diethylstilbestrol were obtained by immunizing rabbits with immune antigen that obtained by conjugated hapten with bovine serumalbum in DES-BSA.The results showed that hexoestrol hapten and bovine serum albumin (BSA) conjugated molar ratio was 12.93∶1.In the future,the resulting antibodies could be used for the detection of indirect competitive enzyme-linked immunosorbent assays for the hexoestrol.

相似文献/References:

[1]于涵,王丽娜,郭东华,等.牛坏死杆菌43K OMP的截短表达及多克隆抗体的制备[J].黑龙江农业科学,2022,(04):68.[doi:10.11942/j.issn1002-2767.2022.04.0068]
　YU Han,WANG Li-na,GUO Dong-hua,et al.Expression and Polyclonal Antibody Preparation of Truncated 43K OMP Protein from Fusobacterium necrophrum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(10):68.[doi:10.11942/j.issn1002-2767.2022.04.0068]

备注/Memo

收稿日期：2016-09-05

基金项目： 哈尔滨市科技攻关计划资助项目（2014AB3BN041）
第一作者简介： 李宁（1979-），女，黑龙江省哈尔滨市人，硕士，高级兽医师，从事饲料及兽药检测研究。 E-mail:liningcatherine@163.com。

更新日期/Last Update: 2016-12-16