[1]鲁珍,谌馥佳,李恩中,等.高产中性蛋白酶菌株的筛选及其产酶条件的研究[J].黑龙江农业科学,2016,(05):110-114.[doi:10.11942/j.issn1002-2767.2016.05.0110]
　LU Zhen,CHEN Fu-jia,LI En-zhong,et al.Screening and Isolation of High Neutral Protease Yielding Strains and Study on the Best Fermentation Performance[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(05):110-114.[doi:10.11942/j.issn1002-2767.2016.05.0110]
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高产中性蛋白酶菌株的筛选及其产酶条件的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2016年05 页码: 110-114 栏目: 出版日期: 2016-05-10
Title:
Screening and Isolation of High Neutral Protease Yielding Strains and Study on the Best Fermentation Performance
文章编号:
1002-2767（2016）05-0110-04
作者:
鲁珍1; 谌馥佳1; 李恩中1; 刘家扬1; 卢自周1; 张建设2; 郜胜勤2
（1.黄淮学院 生物工程系，河南 驻马店 463000；2.河南豫坡酒业有限责任公司，河南 西平 463900）
Author(s):
LU Zhen1; CHEN Fu-jia1; LI En-zhong1; LIU Jia-yang1; LU Zi-zhou1; ZHANG Jian-she2; GAO Sheng-qin2
(1.Biological Engineering Department of Huanghuai University,Zhumadian,Henan 463000; 2.Henan Yupo Limited Company,Zhumadian,Henan 463900)
关键词:
微生物; 酱香菌; 中性蛋白酶; 酶活
Keywords:
microorganism;  sauce bacterium;  neutral protease;  enzyme activity
分类号:
Q939.9
DOI:
10.11942/j.issn1002-2767.2016.05.0110
文献标志码:
A
摘要:
为从高温大曲中筛选出高产中性蛋白酶菌株，利用生理生化实验和16S rDNA序列同源性鉴定和分析产酶条件。结查表明：从已获得的8株产酱香菌株筛选得到高产中性蛋白酶菌株为GX03（706.13 U?g-1），并鉴定该菌株为解淀粉芽孢杆菌。通过单因素和正交试验得到最适产酶条件：含水量为35%，接种量为11%，氮源为硝酸钠，初始pH 5.5，培养时间为6 d，经优化后该菌产酶性能大幅度提高，可达567.30 U?g-1。
Abstract:
In order to extract the strains with high yield neutral protease from high temperature Daqu，enzyme production conditions were studied and authenticated.8 Jiangxiang bacterial strains were isolated from the high temperature Daqu before，and the strain GX03 with high-yield of neutral protease was isolated from the 8 Jiangxiang bacterial strains.It was identified as Bacillus amyloliquefaciens through the physiological and biochemical characteristics and molecular identification.The optimal condition for the fermentation were as follows:moisture content 35%,the strain inoculation amount 11%,NaNO3 as nitrogen source,the initial pH 5.5,fermentation time for 6 days.Under this conditon,the neutral protease was 567.30 U?g-1.

参考文献/References:

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备注/Memo

收稿日期：2016-04-01

基金项目：国家青年自然科学基金资助项目（51503074）；2015年河南省重点科技攻关资助项目（152102210025）
第一作者简介：鲁珍（1987-），女，硕士，助教，从事食品科学研究。E-mail:lzz937272481@163.com。

更新日期/Last Update: 2016-12-11