ZHANG Ni-wen,WU Yue,WU Yun,et al.Effect of Na2S2O3 and Citric Acid on PPO Activity of Annotinous Branch of Magnolia dawsoniana[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(04):68-71.[doi:10.11942/j.issn1002-2767.2016.04.0068]
Na2S2O3和柠檬酸处理对康定木兰一年生枝多酚氧化酶活性的影响
- Title:
- Effect of Na2S2O3 and Citric Acid on PPO Activity of Annotinous Branch of Magnolia dawsoniana
- 文章编号:
- 1002-2767（2016）04-0068-04
- 关键词:
- 康定木兰; 多酚氧化酶活性PPO; Na2S2O3; 柠檬酸
- Keywords:
- Magnolia dawsoniana; PPO activity; Na2S2O3; citric acid
- 分类号:
- Q948
- 文献标志码:
- A
- 摘要:
- 以康定木兰一年生枝条为实验材料，采用不同浓度的Na2S2O3溶液和柠檬酸溶液对枝条进行处理，测定其多酚氧化酶（PPO）活性及其变化规律。结果表明：不同Na2S2O3和柠檬酸处理浓度均对康定木兰枝条PPO活性具有抑制作用，其中1.0 g?L-1 Na2S2O3和0.2 g?L-1柠檬酸处理对康定木兰枝条中PPO活性的抑制效果最佳；随着处理时间的增加，PPO活性亦呈现先下降后上升的趋势，处理6 h时的抑制作用最强。综合来看，1.0 g?L-1Na2S2O3或0.2 g?L-1柠檬酸溶液处理6 h对康定木兰枝条PPO活性的抑制效果最好。
- Abstract:
- The effect of different concentration of Na2S2O3 and citric acid solution on PPO activity and its variations with time of Magnolia dawsonianawas studied.The results showed that PPO activity reduced significantly under the treatments of Na2S2O3 and citric acid.The lowest PPO activity was when the concentration was 1.0 g?L-1 and 0.2 g?L-1 for Na2S2O3 and citric acid,respectively.Similar with the concentration treatments,with the rising of time,PPO activity was reduce-increase trend,the lowest was 6 h treatment.In a word,might carefully make conclusion that concentration of 1.0 g?L-1 Na2S2O3,0.2 g?L-1 citric acid and 6 h treatment bestly reduced PPO activity of Magnolia dawsoniana.
参考文献/References:
[1]
李捷
.
木兰科植物的分支分析
[J].
云南植物研究
,1997,19(4):342-356.
[2]徐凤霞.木兰科植物的分支分析[J].热带亚热带植物学报,1997，8(3):207-214. [3]刘玉壶,周仁章.中国木兰科植物及濒危种类的引种繁殖研究初报[J].植物引种驯化集刊,1987 (5): 39-41. [4]Gooding P S,Bird C,Robinson S P.Molecular cloning and charaterisation of banana fruit Polyphenol oxidase[J].Planta,2001,213(5): 748. [5]傅立国,金鉴明.中国植物红皮书[M].北京:科学出版社,1992：404-455. [6]刘玉壶,夏念和,杨惠秋.木兰科的起源进化和地理分布[J].热带亚热带植物学报,1995,3(4): 1-12. [7]程永现,周俊,丁中涛.粗梗木莲的酚性成分[J] .云南植物研究,2000,22(3) : 365- 367. [8]黎明,马焕成.木兰科植物无性繁殖研究概况[J].西南林学院学报,2003（2）: 92-96. [9]黄海欣.河南辛夷生长习性观察[J].中药材,1990（4）:3-6. [10]王曼玲,胡中立,周明全,等.植物多酚氧化酶的研究进展[J].植物学通报,2005,22(2):215-222. [11]周丽艳,郭振清,秦子禹,等.白玉兰组织培养中的褐化控制[J].河北科技师范学院学报,2008（4）:19-22.
备注/Memo
收稿日期：2016-02-25