ZHANG Ming-hui,ZHANG Yu-zhu,SUN Dong-mei.Identifiction of Rhizoctonia solani?Kühn’s Anastomosis Groups and Its Infection Observation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(03):51-53,54.[doi:10.11942/j.issn1002-2767.2016.03.0051]
马铃薯立枯丝核菌融合群的确定及其对马铃薯侵染的观察
- Title:
- Identifiction of Rhizoctonia solani?Kühn’s Anastomosis Groups and Its Infection Observation
- 文章编号:
- 1002-2767(2016)03-0051-03
- 分类号:
- S435.32
- 文献标志码:
- A
- 摘要:
- 为了研究不同地区马铃薯立枯丝核融合群的变化,对不同取样地的马铃薯立枯丝核菌进行了分离培养。通过番红O-KOH 染色观察,确定分离获得的丝核菌菌株均为多核菌丝体。对获得的35株分离株与标准菌株进行对峙培养,利用菌丝融合试验判断其融合群,发现丝核菌菌株主要归属于AG4融合群;并在显微镜下观察到丝核菌菌丝对马铃薯的侵染。
- Abstract:
- In order to research the change of Rhizoctonia solani?Kühn′s anastomosis groups,the strains of different areas were collected.Through safranin OKOH staining,almost all of these fungi belonged to multinucleate mycelium.The results showed that the obtained 35 strains of R.solani belonged to AG4 anastomosis groups,by the means of bi-culture with standard strains,the hyphae infection of potato were observed by R.solani?through microscope.
参考文献/References:
[1]北京农业大学植保系植物病理生态研究室.植物根际生态学与根病生物防治进展[M].北京:中国人民大学出版社,1991:498-517.
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备注/Memo
收稿日期:2016-01-18