[1]刘畅,卜海东,冯章丽,等.果树授粉蜂种凹唇壁蜂体内生理指标与抗寒性之间的关系[J].黑龙江农业科学,2016,(02):72-76.[doi:10.11942/j.issn1002-2767.2016.02.0072]
　LIU Chang,BU Hai dong,FENG Zhang li,et al.Relationship Between Physiological Indicators and Cold Resistance on Fruit Pollinators Osmia excovata Alfken[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(02):72-76.[doi:10.11942/j.issn1002-2767.2016.02.0072]
点击复制

果树授粉蜂种凹唇壁蜂体内生理指标与抗寒性之间的关系

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2016年02 页码: 72-76 栏目: 出版日期: 2016-02-10
Title:
Relationship Between Physiological Indicators and Cold Resistance on Fruit Pollinators Osmia excovata Alfken
文章编号:
1002-2767（2016）02-0072-05
作者:
刘畅1; 卜海东1; 冯章丽1; 顾广军1; 张武杰1; 曹阳2; 赵德英3
1.黑龙江省农业科学院 牡丹江分院，黑龙江 牡丹江 157041；2.黑龙江省农业经济职业学院，黑龙江 牡丹江 157041；3.中国农业科学院 果树研究所，辽宁 兴城 125100
Author(s):
LIU Chang1; BU Haidong1; FENG Zhangli1; GU Guangjun1; ZHANG Wujie1; CAO Yang2; ZHAO Deying3
1.Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences,Mudanjiang,Heilongjiang 157041；2.Heilongjiang Agricultural Economy Vocational College,Mudanjiang,Heilongjiang 157041；3.Institute of Polomogy of CAAS,Xingcheng,Liaoning 125100
关键词:
凹唇壁蜂; 过冷却点; 体液冰点; 过氧化氢酶; 过氧化物酶; 超氧化物歧化酶
Keywords:
Osmia excovata Alfken; supercooling point;  humor freesing point;  catalase; peroxidase;  superoxidase
分类号:
S66
DOI:
10.11942/j.issn1002-2767.2016.02.0072
文献标志码:
A
摘要:
为进一步研究凹唇壁蜂低温下安全越冬技术，通过对凹唇壁蜂体内生理指标检测，初步研究了生理指标变化与抗寒性之间的关系。结果表明：凹唇壁蜂体内的含水量与体内各种酶含量的变化与抗寒性之间没有直接联系，而氨基酸、脂肪、蛋白质和甘油随着温度的逐渐降低，有逐渐增加并积累的趋势，在凹唇壁蜂的越冬过程中起促进作用，是重要的抗寒物质。同时在人工模拟条件下计算凹唇壁蜂的过冷却点和体液冰点，温度分别为雄性-24.46和-19.41℃，雌性-25.02℃和-19.97℃。本地的最低极端温度能达到-30℃以下，说明凹唇壁蜂在室外不能安全越冬。采取保护措施可以使凹唇壁蜂在本地安全越冬，安全温度应该是-15～-10℃，而决不能低于-18℃。
Abstract:
In order to research the safe wintering technology at low temperature of Osmia excovata Alfken, through the excovata in vivo physiological index detection，a preliminary study of the relationship between physiological changes and cold tolerance was analyzed.The results showed that the change of moisture content and different enzyme content did not have a direct link with the cold resistance.And amino acids,fats,proteins and glycerine with decreasing temperature,there was an increasing trend and gradually accumulated which played a catalytic role in the excovata wintering process,it was an important cold substances.Meanwhile in artificial conditions,the Osmia excavata supercooling points(SCP)and humor freezing point(HFP) were determinated,that indicated that the winter minimum supercooling point and humor freezing point of male Osmia excavata were -24.46 and -19.41℃,-25.02 and -19.97℃ for female Osmia excavata.As the lowest temperature of the local areas -30℃,Osmia excavata could not survive safely the winter.Taking protective measures could make Osmia excovata in the local security,safety temperature should be between -15~-10 ℃,and must not be lower than -18℃.

参考文献/References:

［1］李海茂,丛斌,李建平,等.壁蜂及其在果树授粉中的应用[J].吉林农业大学学报，2004,26(4):422-425. [2]前田泰生,北村泰三.マメコバチ手用人工巢の開凳[J].畏野果榭银告，1983,12(1):24-30. [3]Torchio P F.Field and Experiment with the pollinator species,Osmia lignaria propinqua cresson,in apple orchards[J].Journal of the Kansas Entomological Society,1982,55(1):136-144. [4]魏枢阁,王韧.利用驯化的角额壁蜂提高苹果树座果率及产量[J].中国果树,1990,15(1):31-32. [5]牛力武.关于牡丹江地区果树产业发展的几点思考[J].黑龙江农业科学，2007(4)：101-102. [6]李周直,沈惠娟,蒋巧根,等.几种昆虫体内保护酶系统活力的研究[J].昆虫学报,1994， 37(4):399-403. [7]王学奎.植物生理生化实验原理和技术[M].北京：高等教育出版社， 2006.

备注/Memo

黑龙江省农业科技创新工程资助项目（2014ZD009）；现代农业产业技术体系建设专项资金资助项目（CARS28）

更新日期/Last Update: 2016-11-11