[1]李 晶,杨洪,刘 崇.病毒宏基因组学的研究概况及应用[J].黑龙江农业科学,2015,(12):182-185.[doi:10.11942/j.issn1002-2767.2015.12.0182]
　LI Jing,YANG Hong-yi,LIU Chong.Research and Application of Viral Metagenomics[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(12):182-185.[doi:10.11942/j.issn1002-2767.2015.12.0182]
点击复制

病毒宏基因组学的研究概况及应用

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年12 页码: 182-185 栏目: 出版日期: 2015-12-10
Title:
Research and Application of Viral Metagenomics
文章编号:
1002-2767（2015）12-0182-04
作者:
李 晶; 杨洪 ; 刘 崇
东北林业大学 生命科学学院，黑龙江 哈尔滨 150040
Author(s):
LI Jing; YANG Hong-yi; LIU Chong
?College of Life Science,Northeast Forestry Univesity,Harbin,Heilongjiang 150040
关键词:
病毒宏基因组学;  高通量测序;  随机引物;  PCR 
Keywords:
viral metagenomics;  high throughput sequencing;  random primers;  PCR
分类号:
S852.65
DOI:
10.11942/j.issn1002-2767.2015.12.0182
文献标志码:
A
摘要:
病毒宏基因组学（Viral metagenomics）是新兴起的研究环境中病毒多样性及其分子生物学信息的技术。此技术已在人类健康、动植物病毒检测、环境中病毒多样性分析方面得到广泛应用，对病毒的系统研究、病原体分析及预防具有重要意义。为拓展病毒宏基因组学的应用范围和现实作用，综述了病毒宏基因组学在动植物健康、环境问题以及病毒研究领域的研究概况及其应用展望。
Abstract:
?Viral metagenomics is the rise of new technique,which research viruses’ diversity in the environment and their molecular biology information.Viral metagenomics was widely used on human health,animal and plant virus detection,environment diversity analysis,the systematic study of the virus,the pathogen analysis and prevention had great significance.In order to extend the application scope and reality function,the research and application prospects of viral metagenomics on animal and plant health,environmental issues,as well as virus general situation were reviewed.

参考文献/References:

［1］Mokili J L,Rohwer F,Dutilh B E.Metagenomics and future perspectives in virus discovery[J].Virology,2012,2(1):63-77.

?[2]何彪,涂长春.病毒宏基因组学的研究现状及应用[J].畜牧兽医学报,2012(12):1865-1870.
?[3]李金松.应用非序列依赖单引物PCR（SISPA-PCR）技术筛查腹泻相关的病毒[D].北京：中国疾病预防控制中心,2010.
?[4]许黎黎,秦川.埃博拉出血热动物模型研究进展\[J\].中国比较医学志,2010,20(9)：67-71.
?[5]Handelsman J,Rondon MR,Brady SF,et al.Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products[J].Chem Biol,1998,5(10):R245-R249.
?[6]Reyes G R,Kim J P.Sequenceindependent,single-primer amplification (SISPA) of complex DNA populations\[J\].Mol Cell Probes，1991,5(6):473-481.
?[7]Froussard P.A random-PCR method (r PCR) to construct whole c DNA library from low amounts of RNA[J].Nucleic Acids Res， 1992,20(11):2900.
?[8]Breitbart M,Rohwer F.Method for discovering novel DNA viruses in blood using viral particle selection and shotgun sequencing[J].Biotechniques,2005,39(5):729-736.
?[9]Djikeng A,Halpin R,Kuzmickas R,et al.Viral genome sequencing by random priming methods[J].BMC Genomics,2008,9(5):1-9.
?[10]王兴春,杨致荣,王敏，等.高通量测序技术及其应用[J].中国生物工程志,2012（1）:109-114.
?[11]Thurber R V,Haynes M,Breitbart M,et al.Laboratory procedures to generate viral metagenomes [J].Nat Protoc,2009,4（4）:470-483.
?[12]Djikeng A,Halpin R,Kuzmickas R,et al.Viral genome sequencing by random priming methods[J].GMC Genomics,2008,9(1)：5.
?[13]Fancello L,Raoult D,Desnues C.Computational tools for viral metagenomics and their application in clinical research[J].Virology,2012，434:162-174.
?[14]Li L,Deng X,Mee E T,et al.Comparing viral metagenomics methods using a highly multiplexed human viral pathogens reagent[J].J Virol Methods,2015， 213:139-46.
?[15]浦绍艳,张鑫磊,蒋太交，等.宏基因组学研究方法及其在病原体检测中的应用\[J\].生物物理学报,2014（1）:3-14.
?[16]Palacios G,Druce J,Du L,et al.A new arenavirus in a cluster of fatal transplantassociated diseases[J] .N Engl J Med，2008， 358(10): 991-998.
?[17]Law J,Jovel J,Patterson J,et al.Identification of hepatotropic viruses from plasma using deep sequencing: a next generation diagnostic tool[J].PLos One,2013,8（4）:e60595.
?[18]单同领.病毒宏基因组学分析儿童和猪肠道病毒群落及23株病毒的初步研究[D].上海：上海交通大学,2011.
?[19]Zhang T,Breitbart M,Lee WH,et al.RNA viral community in human feces: prevalence of plant pathogenic viruses[J]. PLos Biol,2006,4(1):e3.
?[20]Willner D,Furlan M,Haynes M,et al.Metagenomic analysis of respiratory tract DNA viral communities in cystic fibrosis and non cystic fibrosis individuals[J].PLos One,2009,4(10):e7370.
[21]Greninger A L,Chen E C,Sittler T,et al.A metagenomic analysis of pandemic influenza A (2009H1N1) Infection in patients from North America[J].PLos One,2010,185(10):e13381.
?[22]Ng TFF,Duffy S,Polston J E,et al.Exploring the diversity of plant DNA viruses and their satellites using vector enabled metagenomics on whiteflies[J].PLos One,2011,6:e19050.
?[23]Li L,Victoria J G,Wang C,et al．Bat guano virome: predominance of dietary viruses from insects and plants plus novel mammalian viruses[J].J Virol,2010,84(14):6955-6965.
?[24]Donaldson E F,Haskew A N,Gates J E,et al．Metagenomic analysis of the viromes of three North American bat species: viral diversity among different bat species that share a common habitat[J].J Virol，2010,84(24):13004-13018.
?[25]杨凡力,王意银,郑文成,等.中国部分地区蝙蝠携带病毒的宏基因组学分析[J].生物工程学报,2013,29(5):586-600.
?[26]Sullivan N J,Sanchez A,Rollin P E,et al.Development of a preventive vaccine for Ebola virus infection in primates[J].Nature,2000,408(6812):605609.
?[27]Rosario K,Nilsson C,Lim Y W,et al.Metagenomic analysis of viruses in reclaimed water[J].Environ Microbiol,2009,11(11):2806-2820.
?[28]Schoenfeld T,Liles M,Wommack K E,et al.Functional viral metagenomics and the next generation of molecular tools[J].Trends Microbiol,2010,18:20-29.
?[29]Willner D,Furlan M,Haynes M,et al.Metagenomic Analysis of Respiratory Tract DNA Viral Communities in Cystic Fibrosis and Non Cystic Fibrosis Individuals[J].PLos One,2009,4(10): e7370.
[30]Maurice ?C F,Haiser H J,Turnbaugh P J.Xenobiotics Shape the Physiology and Gene Expression of the Active Human Gut Microbiome [J].Cell,2013,152(1):39-50.

备注/Memo

收稿日期：2015-09-18

第一作者简介：李晶（1989-），女，黑龙江省青冈县人，在读硕士，从事微生物研究。Email:1401787417@qq.com

更新日期/Last Update: 2017-02-06