HUANG Yan-ning,XIE Jin,FAN Hai-shan,et al.Effect of Matrix Physical and Chemical Properties on Transplanting Survival and Growth of Tin Caulis Dendrobium officinale Kimura et Migo Somaclone[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(10):141-145.[doi:10.11942/j.issn1002-2767.2015.10.0141]
基质理化性质对铁皮石斛组培苗移栽成活及生长的影响
- Title:
- Effect of Matrix Physical and Chemical Properties on Transplanting Survival and Growth of Tin Caulis Dendrobium officinale Kimura et Migo Somaclone
- 文章编号:
- 1002-2767（2015）10-0141-05
- Keywords:
- Dendrobium officinale Kimura et Migo; ; in vitro; culture substrate; physicochemical characteristic
- 分类号:
- S567.239
- 文献标志码:
- A
- 摘要:
- 基质理化性质是影响组培苗移栽时生根成活的关键因素，通过不同基质种类的不同比例配比，对铁皮石斛培养基质的理化性质测定和调查组培苗成活率等指标进行研究。结果表明：铁皮石斛组培苗在各配方的生根基质中较适宜的理化性质配方为锯木∶泥炭∶珍珠岩=1∶2∶1，其容重0.11 g?cm-3，总孔隙度60.58%，毛管孔隙度14.25%，非毛管孔隙度71.56%，大小孔隙比1∶1.3。基质中的氮磷钾养分含量分别为0.059、0.016、0.153 g?kg-1，pH6.8，电导率为0.90 Ec。铁皮石斛在不同基质栽培中的成活率和生根、生长有显著影响，栽培于锯木∶泥炭∶珍珠岩=1∶2∶1基质中的组培苗的成活率、生根情况和生长情况均显著高于其它基质配方，是一种比较适合铁皮石斛组培苗生长的栽培基质。
- Abstract:
- The physical and chemical properties of matrix are the key factors for the somaclone took root transplanting survival.Through the ratio of different proportions of various matrix,determination of matriz physical and chemical properties of Dendrobium officinale Kimura et Migo and survey the somaclone indices such as survival rate were studied.The results showed that the Dendrobium officinale Kimura et Migo somaclone took root in the formula of the matrix was suitable in the physical and chemical properties of the formula for lumber: peat: pearlite was 1:2:1,its bulk density was 0.11 g?cm-3,60.58% of the total porosity and capillary porosity were 14.25% and 71.56%,the capillary porosity size void ratio was 1:1.3.Matrix of NPK nutrient content were 0.059,0.016 and 0.153 g?kg-1,pH value was 6.8,the conductivity was 0.90 Ec.The survival rate,rooting and growth of Dendrobium officinale Kimura et Migo somaclone grew in sawing wood,peat,perlite of 1∶2∶1 matrix which were significantly higher than other matrix formula.It was more suitable for the Dendrobium officinale Kimura et Migo somaclone growth of cultivation matrix.
参考文献/References:
［1］洪森荣.“救命仙草"铁皮石斛研究进展[J].生物学教学2010,11(35):6-7.
[2] 席刚俊,赵桂华.铁皮石斛栽培技术概况[J].安徽农业科学，2011,39(32)：19740-19742.
[3] 段俊，吴坤林.皮石斛生产及问题探讨[J].花卉专业论坛，2011(12):18-19.
[4] 丁长春,浅议铁皮石斛种苗工厂化组培生产与驯化移栽技术[J].文山学院学报,2012,12,25(6):35-36.
[5] 万琳,肖昌泰,毛昆明.优质铁皮石斛栽培基质及其营养研究进展[J].云南农业,2012(5):27-29.
[6] 潘颖,李孝良.几种无土栽培基质理化性质比较[J].安徽农学通报,2007,13(5):55-56.
[7] 赵九洲,陈洁敏,王义庆,等.六种无土栽培代用基质理化特性的比较[J].莱阳农学院学报，2001,18(3)：161-164,200.
[8] 李静,赵秀兰,魏世强,等.无公害蔬菜无土栽培基质理化特性研究[J].西南农业大学学报,2000,4,22(2):112-115.
[9] 尹淑莲,郭伟珍,林艳,等.基质理化性质对组培苗生根状况的影响[J].辽宁林业科技,2005(5):18-19,24.
[10] 齐海鹰,张淑霞,宋朝玉,等.不同基质和供水方式对基质理化特性和一品红观赏品质的影响[J].植物资源与环境学报,2009,18(3):74-80.
[11] 张沛健,谢耀坚,彭彦,等.桉树皮基质理化性质变化对苗木生长的影响[J].广东农业科学，2011(10):59-61,72.
[12] 潘颖,李孝良.几种无土栽培基质理化性质比较[J].安徽农学通报,2007,13(5)：55-56.
[13] 童贯和,张科贵,刘天骄,等.新型无土栽培基质配比对4种叶菜类蔬菜生长发育和产量的影响——以煤矸石、油菜秸秆等组成的栽培基质为例[J].农业现代化研究,2012,33(6): 762-765.
[14] 陈晨甜,吕长平,陈建,等.不同配比混合基质对非洲菊生长和开花的影响[J].湖南农业大学学报：自然科学版,2009(6):656-659.
[15] 王欢妍,黄科,高琪昕,等.马铃薯微型薯繁育基质配比的优化[J].湖南农业大学学报：自然科学版,2013 (5):505-509.
[16] 吴志华．花卉生产技术[M].北京：中国林业出版社，2003.
相似文献/References:
[1]曹天旭,宿肇辉,韩超慧.铁皮石斛组培快繁技术的研究[J].黑龙江农业科学,2014,(05):30.
CAO Tian-xu,SU Zhao-hui,HAN Chao-hui.Study on Tissue Culture and Rapid Propagation of Dendrobium candidum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):30.
[2]黎 萍,刘连军,彭靖茹,等.多效唑在木薯组织培养中的应用研究[J].黑龙江农业科学,2012,(10):61.
LI Ping,LIU Lian-Jun,PENG Jing-ru,et al.Study on Application of Paclobutrazol in Tissue Culture of Cassava[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(10):61.
[3]石晓云,王僧虎.马铃薯组培苗壮苗培养的研究[J].黑龙江农业科学,2010,(06):11.
SHI Xiao-yun,WANG Seng-hu.Research on Strong Seedlings Culture of Potato Tissue Culture Seedlings[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):11.
[4]尹明华,聂凤琴,胡文韬,等.NAA和KT对铁皮石斛原球茎增殖与分化的影响[J].黑龙江农业科学,2011,(08):25.
YIN Ming-hua,NIE Feng-qin,HU Wen-tao,et al.Effects of KT and NAA on the Proliferation andDifferentiation of Dendrobium candidum Protocorm[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(10):25.
[5]李景云.铁皮石斛组织培养研究进展[J].黑龙江农业科学,2013,(12):148.
LI Jing-yun.Advance on Tissue Culture of Dendrobium Candidum Wall. Ex Lindl.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(10):148.
[6]王华宇,陈乃明,何贵整,等.玉簪属植物工厂化组培育苗关键技术概述[J].黑龙江农业科学,2013,(12):150.
WANG Hua-yu,CHEN Nai-ming,HE Gui-zheng,et al.Summary of Key Techniques of Industrial Tissue Culture Seedling for Hosta Plants[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(10):150.
[7]桂明春,管艳,田海,等.星点木组培芽扦插技术研究[J].黑龙江农业科学,2015,(01):85.[doi:10.11942/j.issn1002-2767.2015.01.0085]
GUI Ming-chun,GUAN Yan,TIAN Hai,et al.Study on Cutting Technique of Tissue Culture Plantlets of Dracaena godseffiana[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(10):85.[doi:10.11942/j.issn1002-2767.2015.01.0085]
[8]刘丽.培养条件对黑木相思组培苗玻璃化的影响[J].黑龙江农业科学,2015,(05):29.[doi:10.11942/j.issn1002-2767.2015.05.0029]
LIU Li.Effect of Culture Conditions on Vitrification of Acacia melanoxylon[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(10):29.[doi:10.11942/j.issn1002-2767.2015.05.0029]
[9]王疆江,刘建新,王秋玉,等.饲料型四倍体刺槐组培苗的遗传学鉴定[J].黑龙江农业科学,2009,(02):7.
WANG Jiang-jiang,LIU Jian-xin,WANG Qiu-yu,et al.The Genetic Evaluation of Plantlets on Fodder-type Tetraploid Robiniapseudoacacia[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(10):7.
[10]陆 兵.铁皮石斛组织培养研究进展[J].黑龙江农业科学,2009,(02):164.
LU Bing.Advance on the Tissue Culture of Dendrobiumcandidum Wall .ex Lindl[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(10):164.
备注/Memo
收稿日期：2015-01-12
基金项目：湖南省农业科学院创新基金资助项目（2011-hnnkycx20）；长沙药用植物特色产业科技示范基地建设资助项目（K1303011-21）
第一作者简介：黄艳宁（1982-），女，湖南省新宁县人，硕士，助理研究员，从事药用植物栽培及种苗快繁研究。E-mail:huangyanning1121@163.com。通讯作者：朱校奇（1961-），男，湖南省岳阳市，博士，研究员，从事药用植物资源与利用研究。E-mail:zhuxiaoqi222@163.com。