[1]王小国.紫色马铃薯试管苗的培养及微型薯的诱导[J].黑龙江农业科学,2015,(06):12-14.[doi:DOI:10.11942/j.issn1002-2767.2015.06.0012]
　WANG Xiao-guo.Study on Testtube Seedlings Cultivation of Purple Potato and Induction of Minituber[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(06):12-14.[doi:DOI:10.11942/j.issn1002-2767.2015.06.0012]
点击复制

紫色马铃薯试管苗的培养及微型薯的诱导

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年06 页码: 12-14 栏目: 出版日期: 2015-06-10
Title:
Study on Testtube Seedlings Cultivation of Purple Potato and Induction of Minituber
文章编号:
1002-2767（2015）06-0012-03
作者:
王小国
三门峡职业技术学院 食品园林学院，河南 三门峡 472000
Author(s):
WANG Xiao-guo

College of Food and Landscape Architecture,Sanmenxia Polytechnic,Sanmenxia,Henan 472000
关键词:
紫色马铃薯; 试管苗; 培养; 微型薯; 诱导
Keywords:
purple potato; tube seedlings;  culture;  minituber;  induction
分类号:
S532
DOI:
DOI:10.11942/j.issn1002-2767.2015.06.0012
文献标志码:
A
摘要:
为促进紫色马铃薯产业的更好发展，以MS为基本培养基,黑金刚马铃薯块茎催芽后的芽体为材料，对外植体的消毒方式和微型薯诱导的影响因素进行了分析。结果表明：先用75%酒精消毒30 s，无菌水冲洗1次，再用0.1%升汞消毒7～8 min，无菌水冲洗3～5次为外植体较好的消毒方式；正交试验结果表明9种培养基对试管薯的诱导结果差异较为明显，除添加3%蔗糖的3种培养基没有诱导出微型薯外，其余培养基均能诱导出微型薯，培养基MS+蔗糖7%+6-BA1.0 mg·L-1+ KH2PO4510 mgL-1+NH4NO3550 mg·L-1上微型薯诱导率最高，为83.33%，MS+蔗糖7%+6-BA 5.0 mg·L-1+ KH2PO4 170 mg.L-1+NH4NO31 650 mg·L-1的培养基上诱导的微型薯直径最大。
Abstract:
In order to promote the development of potato industry better,taking MS as the basic culture medium and Heijingang potato tuber buds as the explants,explants disinfection method and the influence factors of minituber inducement was analyzed .The results showed that the better way to disinfect explants was 75% alcohol disinfect for 30 s firstly,sterile water flushing,0.1% HgCl2 disinfect for 7~8 min and sterile water flushing for 3~5 times; Orthogonal test showed that 9 kinds of medium could induce minituber except medium containing 3% sucrose,the induction rate was the highest on the MS+ sucrose 7%+6-BA1.0 mg〖DK1〗·L-1+KH2PO4510 mg·L-1+NH-4NO-3550 mg·L-1 (83.33%),the minituber diameter was largest on the medium MS+sucrose 7%+6-BA5.0 mg〖DK1〗·L-1+ KH2PO4170 mg·L-1+NH4NO31 650 mg·L-1.

参考文献/References:

［1］田宏,王敬锋.“黑薯”创造大效益[J].吉林农业,2014(14):4.

[2]杨智勇,李新生,马娇燕,等.紫色马铃薯“黑金刚”中花青苷组分和含量分析[J].食品科学,2013(14):271-275.

[3]岳红,卢其能,赵昶灵,等.蔗糖浓度和外源激素对马铃薯微型薯诱导的影响[J].江苏农业科学,2012,40(7):58-60.

[4]赵燕,刘清波,胡清云,等.五种基因型马铃薯微型薯诱导研究[J].黑龙江农业科学,2012(1):22-25.

[5]张瑜琨,海丽,杨茹薇,等.两种培养基培育脱毒试管苗对微型薯产量的影响[J].农村科技,2014(3):13-14.

[6]金辉.密度、季节和种源对马铃薯微型薯繁育影响的研究[D].武汉：华中农业大学,2012.

[7]郑敏.多效唑对马铃薯试管薯形成的影响[J].陕西农业科学,2012,58(3):56-58.

[8]肖旭峰,刘明月.不同生长调节剂对马铃薯微型薯结薯的影响[J].中国马铃薯,2008,22(1):14-16.

[9]陈巧玲,曾黎辉.‘红皮’马铃薯试管苗培养和微型薯诱导[J].亚热带植物科学,2011,40(2):47-49.

[10]史俊,王永平.蔗糖浓度及光照对马铃薯微型薯诱导的影响[J].安徽农学通报,2009,15(14):34-35,159.

[11]徐邦会,姜晓峰,周殿革，等.脱毒马铃薯试管苗快繁及微型薯诱导技术[J].种子世界,2011(4):40-42.

[12]丁运华,李桥文,陈兆贵，等.马铃薯茎尖试管苗及微型试管薯繁育的影响因子研究[J].中国马铃薯,2008,22(2):91-93.

[13]王红梅,李淑洁,张正英，等.不同基因型马铃薯试管薯的诱导及其再生研究[J].甘肃农业大学学报,2008,43(5):55-58.

相似文献/References:

[1]吕俊俐,荆婷,张瑜,等.耐寒茵陈蒿试管苗培养的研究[J].黑龙江农业科学,2013,(02):10.
　LYU Jun-li,JING Ting,ZHANG Yu,et al.Study on Clone Establishment of Frigostabile Artemisia capilaris[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):10.
[2]陈 富,杨 谋,马廷蕊,等.马铃薯试管苗分层立体栽培繁育脱毒微型种薯[J].黑龙江农业科学,2012,(06):27.
　CHEN Fu,YANG Mou,MA Ting-rui,et al.Plantlets Layered Hierarchical Cultivation toBreed Free-Virus Potato Minituber[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):27.
[3]孙婧靓,孙胜香,刘强强,等.ZT对地被菊品种——醉西施试管苗的影响[J].黑龙江农业科学,2013,(06):8.
　SUNJing-liang,SUNSheng-xiang,LIUQiang-qiang,et al.EffectofZTontheGrowthof Chrysanthemum morifolium Ramat invitro[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):8.
[4]邱广伟,夏平,夏静波,等.紫色马铃薯茎尖培养基筛选试验[J].黑龙江农业科学,2011,(03):24.
　QIU Guang-wei,XIA Ping,XIA Jing-bo,et al.Selection of Stem Culture Medium of Purple Potato[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(06):24.
[5]曲艺姣,周清,孙佳,等.美国紫薯试管苗微型繁殖的研究[J].黑龙江农业科学,2013,(07):10.
　QU Yi-jiao,ZHOU Qing,SUN Jia,et al.Study on Micro Propagation of Tube Seedlings of the Ipomoea batatas[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):10.
[6]夏静波,夏 平,邱广伟,等.活性炭在马铃薯组织培养中的应用[J].黑龙江农业科学,2011,(05):16.
　XIA Jing-bo,XIA Ping,QIU Guang-wei,et al.Application of Activated Carbon in Potatoes Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(06):16.
[7]刘书宇,刘 祥,朱 明,等.紫薯试管苗热处理的效应[J].黑龙江农业科学,2011,(11):10.
　LIU Shu-yu,LIU Xiang,ZHU Ming,et al.Effect of Heat Treatment on Test-tube Seedlings of Ipomoea batatas[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(06):10.
[8]赵玉芬,储博彦,刘满光,等.卡拉胶对大花萱草试管苗生长的影响[J].黑龙江农业科学,2015,(10):24.[doi:10.11942/j.issn1002-2767.2015.10.0024]
　ZHAO Yu-fen,CHU Bo-yan,LIU Man-guang,et al.Effect of Carrageenan on the Tube Seedings of Hemerocallis hybridas [J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(06):24.[doi:10.11942/j.issn1002-2767.2015.10.0024]
[9]周淑芹,朱光新,苏俊,等. 应用电镜技术对试管保存马铃薯毒源效果的鉴定研究[J].黑龙江农业科学,1995,(03):41.[doi:10.11942/j.issn1002-2767.1995.03.0041]
　[J].HEILONGJIANG AGRICULTURAL SCIENCES,1995,(06):41.[doi:10.11942/j.issn1002-2767.1995.03.0041]
[10]陆　兵.铁皮石斛组织培养研究进展[J].黑龙江农业科学,2009,(02):164.
　LU Bing.Advance on the Tissue Culture of Dendrobiumcandidum Wall .ex Lindl[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(06):164.

备注/Memo

收稿日期：2015-01-04

基金项目：三门峡职业技术学院技术应用资助项目（SZY-2013-017）

作者简介：王小国（1977-），男，山西省长治市人，硕士，讲师，从事生物技术及其应用研究。E-mail：314087922@qq.com。

更新日期/Last Update: 2015-07-13