YU Yang,JIANG Shi-cui,WANG Kang-yu,et al.Research Progress of Large Fragment DNA Cloning Vector[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(02):147-150.[doi:10.11942/j.issn1002-2767.2015.02.0147]
大片段DNA克隆载体的研究进展
- Title:
- Research Progress of Large Fragment DNA Cloning Vector
- 文章编号:
- 1002-2767（2015）02-0147-04
- Keywords:
- cloning vector; BIBAC; large fragment DNA
- 分类号:
- Q819
- 文献标志码:
- A
- 摘要:
- DNA克隆技术是分子生物学和基因工程研究中非常重要的一项技术。自第一个质粒载体pSC101作为第一个克隆载体以来，随着分子生物学技术的迅猛发展克隆载体的整体结构、容载能力和转化效率都有了很大的改善。通过综述克隆载体的发展概况，以及大片段DNA文库的构建和应用，对大片段DNA遗传转化的发展做了展望。
- Abstract:
- DNA cloning techniques is a very important technology in molecular biology and genetic engineering research.Since the first pSC101 plasmid cloning vector as the first cloning vector,the rapid development of molecular biology techniques,the overall structure of the cloning vector,the capacity-load capacity and conversion efficiency had been greatly improved.Through the reviews of the development situation of the cloning vector,as well as construction and application of large fragments of DNA libraries,and large pieces of DNA genetic transformation of development were put forward.
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备注/Memo
收稿日期：2014-12-20
基金项目：国家高技术研究发展计划（863计划）资助项目（2013AA102604-3）
第一作者简介： 于洋（1988-），女，吉林省长春市人，在读硕士，从事植物分子生物学与基因工程研究。E-mail：835312991@qq.com。
通讯作者：王义（1964-），男，博士，教授，硕士生导师，从事药用植物细胞工程研究。E-mail：wanglaoshi2007@tom.com。