MA Huai-liang,CHEN Huan,MI Chun-xia,et al.Effects of High Temperature Stewing Time and Sodium Carbonate or Sodium Bicarbonate Concentrations on Cell Disruption of Auricularia auricula Judae[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(02):115-117.[doi:10.11942/j.issn1002-2767.2015.02.0115]
高温蒸煮时间和碳酸(氢)钠浓度对黑木耳破壁的影响
- Title:
- Effects of High Temperature Stewing Time and Sodium Carbonate or Sodium Bicarbonate Concentrations on Cell Disruption of Auricularia auricula Judae
- 文章编号:
- 1002-2767(2015)02-0115-03
- Keywords:
- stewing; sodium carbonate; sodium bicarbonate; cell disruption; Auricularia auricula Judae
- 分类号:
- S646.6
- 文献标志码:
- A
- 摘要:
- 为有效地提取黑木耳中的成分以利于黑木耳深层次开发,以可溶性物质含量为指标,应用均匀设计法研究了高温蒸煮(121℃)时间(20~120 min)、碳酸钠和碳酸氢钠浓度(0~0.5%)对黑木耳破壁的影响。结果表明:可溶性物质(y)与Na2CO3浓度(x1)和时间(x3)以及NaHCO3浓度(x2)呈函数关系,即y=-1.392 1+135.864 4x1+0.101 9x2-70.209 6x12(R2=0.992 8,P<0.01);y=12.957 2+154.161 7x22(R2=0.902 2,P<0.01)。根据方程理论计算和实际验证,Na2CO3浓度为0.5%,时间为120 min,破壁效果最好;NaHCO3浓度为0.5%,破壁效果最好。
- Abstract:
- In order to effectively extract ingredients and lay a foundation for deep development for fruit bodies of Auricularia auricula Judae,the method of uniform design was applied to study the effects of high temperature(121℃)stewing time(20~120 min)and concentrations of sodium carbonate or sodium bicarbonate (0~0.5%)on cell disruption of fruit bodies indicated by solute contents.The results showed that solute contents (y) had a functional relation with time and concentrations of sodium carbonate,and concentrations of sodium bicarbonate,y=-1.3921+135.864 4x1+0.101 9x2-70.209 6x12(R2=0.992 8,P<0.01), y=12.957 2+154.161 7x22(R2=0.902 2, P<0.01).Based on theoretical calculations of equations and further actual verifications, the optimum conditions of cell disruption were 120 min and the sodium carbonate concentration was 0.5%; sodium bicarbonate concentration was 0.5%, respectively.
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备注/Memo
收稿日期:2014-08-10
基金项目:牡丹江师范学院资助项目(QY201220)
第一作者简介: 马怀良(1976-),男,黑龙江省牡丹江市人,在读博士,副教授,从事应用微生物研究。E-mail:mzhuailiang@yeah.net。