WANG Peng-liang,YANG Li-ping,LI Tie-ying,et al.In Silico Cloning and Bioinformatic Analysisof EcWRKY75 in Eucalyptus camaldulensis [J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):28-32.
赤桉EcWRKY75基因的电子克隆和生物信息学分析
- Title:
- In Silico Cloning and Bioinformatic Analysisof EcWRKY75 in Eucalyptus camaldulensis
- 文章编号:
- 1002-2767（2014）12-0028-05
- 分类号:
- S792.39
- 文献标志码:
- A
- 摘要:
- WRKY75基因在植物的生长发育以及生物和非生物胁迫过程中发挥着重要作用，对其遗传改良进行研究极为必要。以赤桉的EST序列为材料，利用电子克隆技术克隆1个与拟南芥中WRKY75高度同源的WRKY转录因子，命名为EcWRKY75，并对其开展生物信息学分析。结果表明：EcWRKY75开放阅读框长为579 bp，编码192个氨基酸，分子量为21.395 KD，等电点为9.72；该基因编码的蛋白包含1个WRKYGQK保守结构域和1个C2H2的结构域，属GroupⅡ成员；该蛋白很可能在细胞核中起转录、转录调控或信号转导等作用。该基因的电子克隆和生物信息学分析为其实验克隆和功能分析提供可靠的参考依据。
- Abstract:
- The gene WRKY75 plays key roles in the development,abiotic stress and biotic stress of plants,it is essential to study.Based on the EST sequences of Eucalyptus camaldulensis,EcWRKY75 was cloned in silico,and its bioinformatic analysis was carried out.The results indicated that EcWRKY75 with a ORF of 579 bp encoded 192 amio acid with the molecular weight of 21.395 KD and the isoelectric point of 9.72;the encoded protein belonged to the Group Ⅱwith a conserved domain of WRKYGQK and a domain of C2H2,the protein participated in transcription,transcription regulation or singnal transduct in the nuclei,which laid a foundation for the identification and functional analysis of EcWRKY75.
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备注/Memo
基金项目：广西优良用材林资源培育重点实验室开放课题基金资助项目(12A0301)；国家高技术研究发展计划(863计划)资助项目(2013AA102705)；钦州市科学研究和技术开发资助项目(20137003)