[1]张勇.玉米SSR分子标记试验体系优化及其遗传多样性研究[J].黑龙江农业科学,2014,(09):1-7.
　ZHANG Yong.System Optimization on Maize SSR Molecular Marker and Research of Genetic Diversity[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(09):1-7.
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玉米SSR分子标记试验体系优化及其遗传多样性研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年09 页码: 1-7 栏目: 出版日期: 2014-09-10
Title:
System Optimization on Maize SSR Molecular Marker and Research of Genetic Diversity
文章编号:
1002-2767（2014）09-0001-07
作者:
张勇
北京林业大学 生物科学与技术学院/北京林业大学林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室，北京 100083
Author(s):
ZHANG Yong
College of Biological Sciences and Biotechnology/National Engineering Laboratory for Tree Breeding/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education，Beijing Forestry University,Beijing 100083
关键词:
SSR; 体系优化; 遗传多样性
Keywords:
SSR markers; system optimization; genetic variation
分类号:
S513.52
文献标志码:
A
摘要:
为优化玉米SSR分子标记体系，丰富种质资源的多样性，以基础玉米自交系CK及其诱变系M为材料，优化了SSR分子标记试验体系，并对诱变玉米自交系遗传多样性进行了分析。结果表明：（1）优化后SSR分子标记试验体系，可以有效提高试验效率，减少试验误差；（2）利用23对SSR引物对诱变玉米自交系进行PCR扩增，共扩增得到59个等位基因，平均每对引物检测到2.565个等位基因；位点的多态信息量（PIC）和基因型多样性指数变化范围为0.110～0.525和0.234～1.061，平均值分别为0.331和0.643，说明基础材料CK与诱变材料存在真实的遗传差异。
Abstract:
In order to optimize the SSR molecular marker system and rich the diversity of germplasm resources, taking maize inbred line CK and its seven mutants as materials, the experiment system was optimized and the genetic difference between mutants and the corresponding basic material was studied based on SSR markers with selected 23 pairs of polymorphism SSR primers.The results showed that(1)The optimization of SSR molecular marker experiment system could effectively improve the efficiency of the experiment,reduce the experimental error;(2)By using the 23 pairs of SSR primer,a total of 59 polymorphic amplified fragments was produced.The average number of allele per SSR locus was 2.565.The polymorphism information content(PIC)for the SSR loci varied from 0.110 to 0.525 with an average of 0.331.The amplitude of genetic diversity indexes ranged from 0.234 to 1.061 with an average of 0.643.The phenomenon indicated that massive genetic variation could appear in maize(CK)progenies due to mutagenesis.

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备注/Memo

收稿日期：2014-03-13
作者简介：张勇(1987-)，男，重庆市开县人，在读硕士，从事植物生物技术研究。E-mail:zhangy_0128@126.com。

更新日期/Last Update: 2014-12-03