[1]孙海龙.金叶连翘组培快繁技术研究[J].黑龙江农业科学,2014,(05):35-37.
SUN Hai-long.Study on Rapid Propagation Technique for Forsythia koreanna by Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(05):35-37.
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SUN Hai-long.Study on Rapid Propagation Technique for Forsythia koreanna by Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(05):35-37.
金叶连翘组培快繁技术研究
《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S]
卷:
期数:
2014年05
页码:
35-37
栏目:
出版日期:
2014-05-10
- Title:
- Study on Rapid Propagation Technique for Forsythia koreanna by Tissue Culture
- 文章编号:
- 1002-2767(2014)05-0035-03
- Keywords:
- Forsythia koreanno ; immature stem; tissue culture
- 分类号:
- S685.24
- 文献标志码:
- A
- 摘要:
- 为了建立金叶连翘组培快繁体系,采用金叶连翘新生枝梢为外植体,采用不同浓度激素处理,研究组培快繁过程中外植体分化、增殖、生根及相关因素对金叶连翘快繁的影响。结果表明:金叶连翘最佳的初代培养体系为MS+6-BA 3 mg·L-1+NAA 0.1 mg·L-1,增殖培养体系为MS+6-BA 2.5 mg·L-1+NAA 0.1 mg·L-1,生根培养体系为1/2MS+NAA 0.2 mg·L-1或1/2MS+IBA 0.2 mg·L-1。
- Abstract:
- In order to establish rapid propagation system for Forsythia koreanna,taking new branch of Forsythia koreanna as explant,Hormone treatmens with different concentration were adopted,the effects of explant differentiation,proliferation,rooting and related factors on rapid propagation of Forsythia koreanna were studied.The results showed that the best primary culture medium was MS+6-BA 3 mg·L-1+NAA 0.1 mg·L-1,and optimal medium for proliferation was MS+6-BA 2.5 mg·L-1+NAA 0.1 mg·L-1,rooting medium was 1/2 MS+NAA 0.2 mg·L-1or 1/2 MS+IBA 0.2 mg·L-1.
参考文献/References:
[1]白雅鹃,章林,陈建军,等.金叶连翘组培快繁技术的研究[J].吉林林业科技,2006,35(1):15-19. [2]马燕,韩瑞超,臧德奎,等.木本观赏植物组织培养研究进展[J].安徽农业科学,2012,40(4):1956-1958,2036.
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[1]顾利利,潘丹丹,唐晓倩,等.红花玫瑰植物组织培养的研究[J].黑龙江农业科学,2014,(12):23.
GU Li-li,PAN Dan-dan,TANG Xiao-qian,et al.Study on Plant Tissue Culture of Red Rose[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(05):23.
备注/Memo
收稿日期:2014-01-15
作者简介: 孙海龙(1981-),男,黑龙江省牡丹江市人,硕士,讲师,从事园林工程现场施工与管理方面研究。E-mail:412760558@qq.com。
更新日期/Last Update:
2014-08-08