GUO Qiang,ZHANG Hong-ji,ZHANG Ju-mei,et al.Polymorphism of Lipoxygenase Genes in Russia Spring Wheat Cultivars[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(05):20-25.
俄引春小麦脂肪氧化酶活性基因的标记分析
- Title:
- Polymorphism of Lipoxygenase Genes in Russia Spring Wheat Cultivars
- 文章编号:
- 1002-2767（2014）05-0020-06
- Keywords:
- Russian spring wheat; lipoxygenase; polymorphism
- 分类号:
- S512.12
- 文献标志码:
- A
- 摘要:
- 为了改良黑龙江省小麦品种，加强其种质创新，利用与脂肪氧化酶基因（LOX）活性有关的2个遗传位点相连锁的3对分子标记，对247份俄罗斯引进的春小麦品种进行研究。结果表明：对于SSR标记Xwmc312，142份品种扩增出247 bp特异条带，即Xwmc312-247基因型比例为57.49%，85份品种扩增出235 bp特异条带，Xwmc312-235基因型比例为34.41%，20份品种扩增出227 bp特异条带，Xwmc312-227基因型比例为8.10%。TaLOX-B1位点上，16份品种扩增出标记LOX16的特异条带，即高活性等位基因型TaLox-B1a分布比例是6.48%，230份品种扩增出标记LOX18特异条带，即低活性的等位基因型TaLOXB1b分布比例是93.12%，有一个品种在此位点表现杂合。表明以TaLox-B1b基因型为主。2个位点不同等位基因组合共有6种，Xwmc312-247/TaLOX-B1b分布比例最高（53.85%），Xwmc312-227/TaLOX-B1a分布比例最低（0.41%），高活性组合Xwmc312-235/TaLOX-B1a（分布比例2.43%）等其它4种组合型介于二者之间，表明俄罗斯引进小麦品种以LOX低活性组合型为主。
- Abstract:
-
In order to improve the wheat varieties of Heilongjiang province,strengthen the germplasm innovation,247 spring wheat varieties from Russian were detected by 3 pairs of molecular markers linked with 2 lipoxygenase（LOX）loci.The results showed that for Qlpx.caas1AL loci,there were 142 cultivars amplified Xwmc312-247 alleles with 247 bp specific bands,85 cultivars amplified Xwmc312-235 with 235 bp specific bands,20 cultivars amplified Xwmc312-227 with 247 bp specific bands for SSRXwmc312,distribution ratio of Xwmc312-247,Xwmc312-235 and Xwmc312-227 were 57.49%,34.41%and 8.10% respectively.For TaLOX-B1 loci,16 cultivars amplified high activity allele specific band(TaLOX-B1a)for markerLox16,230 cultivars amplified low activity allele specific band(TaLOX-B1b )for marker Lox18,1 cultivar was hybrid,distribution ratio of TaLOX-B1a and TaLOX-B1b were 6.48% and 93.12% which suggested that TaLOX-B1b was the most in TaLOX-B1 loci.There were six allelic combinations of in 2 loci,Xwmc312-247/TaLOX-B1b distribution ratio was the most(53.85%),and that distribution ratio of Xwmc312-225/TaLOX-B1a was least(0.41%).Other four allelic combinations were the medium.The data indicated that spring wheat varieties introduced from Russian was based on the combination of low LOX activity.
参考文献/References:
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备注/Memo
收稿日期：2014-02-21 基金项目：国家国际科技合作资助项目（2011DFR30840-4） 通讯作者：张宏纪（1969-），男，黑龙江省抚远县人，研究员，从事小麦诱变与生物技术育种研究。Email:fumai@163.com。
第一作者简介：郭强（1969-），男，黑龙江省绥化市人，在读硕士，农艺师，从事作物新品种推广与高产栽培技术研究。E-mail:fumai@163.com。