[1]范国权,白艳菊,高艳玲,等.我国马铃薯主产区病毒病发生情况调查[J].黑龙江农业科学,2014,(03):68-72.
　FAN Guo quan,BAI Yan ju,GAO Yan ling,et al.Investigation on Virus Disease of the Main Potato Producing Areas in China[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):68-72.
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我国马铃薯主产区病毒病发生情况调查

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年03 页码: 68-72 栏目: 出版日期: 2014-03-10
Title:
Investigation on Virus Disease of the Main Potato Producing Areas in China
文章编号:
1002-2767（2014）03-0068-05
作者:
范国权; 白艳菊; 高艳玲; 张威; 张抒; 申宇; 李学湛
黑龙江省农业科学院 植物脱毒苗木研究所，黑龙江 哈尔滨 150086
Author(s):
FAN Guoquan; BAI Yanju; GAO Yanling; ZHANG Wei; ZHANG Shu; SHEN Yu; LI Xuezhan
Virusfree Seeding Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086
关键词:
马铃薯病毒病; 普查; 侵染率; DASELISA
Keywords:
potato viruses; investigation; infection rate; DASELISA
分类号:
S435.32
文献标志码:
A
摘要:
为了解我国马铃薯病毒病发生情况，在马铃薯主产区黑龙江、内蒙古、甘肃、云南等马铃薯田采集了具有典型病毒病症状的样品、疑似样品和随机无症样品，试管苗和原原种为随机样品，共649份。应用DASELISA方法筛查6种马铃薯主要病毒：PVX、PVY、PVS、PLRV、PVM和PVA。结果表明:129份样品为阳性，PVY的检出率最高，为9.86%，PVS次之，为6.47%；试管苗PVS检出最多，为32份，原原种PVX检出最多，为10份，大田样品PVY病毒发生比例最高，为54份；有38份样品是由多种病毒复合侵染造成的，大田PVY+PVS复合侵染率最高，为2.93%，PVS+PVY+PLRV次之，为0.98%，试管苗PVS+PVM复合侵染率最高，为4.85%。通过历年的检测结果比较，试管苗中PVS病毒检出率最高，原原种中PVX和PLRV病毒成为危害最为严重的病害，大田样品中PVY检出率一直居高不下。
Abstract:
In order to investigate the occurrence of viruses on potato around China,649 samples were collected in main potato producing areas including Heilongjiang,Inner Mongolia,Gansu and Yunnan province.Six viruses of PVX,PVY,PVS,PVM,PVA and PLRV were tested by DASELISA.The results showed that there were 129 copies of the samples which were tested as positive,PVY had the highest detection rate which was 9.86%,and PVS followed by 6.47% while PVS was the highest detection in the virusfree plantlets which was 32 samples,PVX was the highest in predlitey,PVY was highest in field which was 54 samples;which was 10 samples. 38 samples were infected by infection with multiple viruses,in the field, compound infection rate was the highest of PVY+PVS which was 2.93%,and PVS+PVY+PLRV was 0.98%.PVS+PVM were compound infection rate in the virusfree plantlets which was 4.85%;Comparing the results of over the years,PVS was the highest detection rate in virusfree plantlets,PVX and PLRV become the most serious disease in preelite,the potao virus incidence of PVY was the highest in field.

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备注/Memo

收稿日期：2013-12-28基金项目：现代农业产业技术体系专项资金资助项目（CARS-10-P14）；“十二五”国家科技支撑计划资助项目（2012BAD06B02）；黑龙江省自然科学基金资助项目（C201234）；黑龙江省农业科技创新工程资助项目(2012QN12) 第一作者简介：范国权（1982-），男，黑龙江省木兰县人，硕士，助理研究员，从事马铃薯病害研究。E-mail：fgq_520@126.com。通讯作者：白艳菊（1972-），女，博士，研究员，从事马铃薯病害研究。E-mail:yanjubai@163.com。

更新日期/Last Update: 2014-09-16