MA Wen-dong,LI Xiu-ping,ZHANG Xian-guo,et al.Study on Determination Method of Amylose Content of Cold Region Rice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):105-108.
寒地水稻直链淀粉含量测定方法的研究
- Title:
- Study on Determination Method of Amylose Content of Cold Region Rice
- 文章编号:
- 1002-2767(2014)02-0105-04
- 关键词:
- 水稻; 直链淀粉含量; 近红外谷物分析仪测定法; 改良的标准测定法; 单粒法
- Keywords:
- rice; amylose content; determination of near infrared grain analyzer; modified standard method; singlegain method
- 分类号:
- S511
- 文献标志码:
- A
- 摘要:
- 水稻直链淀粉含量是水稻品质育种的重要衡量指标,为加速水稻品质育种进程,以10份寒地水稻品种为试验材料,采用近红外谷物分析仪测定法、改良的标准测定法以及单粒法3种测定方法分别对精米粒、糙米粒、精米粉和糙米粉进行直链淀粉含量的测定。结果表明:3种测定方法适用范围不同,且具有各自的分析特点。单粒法测定结果与改良的标准测定法结果平行可靠,可为水稻直链淀粉含量遗传分析和早期世代选择提供有效的测定方法,根据单粒法对糙米粉直链淀粉含量的测定结果重新建立了分级标准。
- Abstract:
- Rice amylose content is an important measurement index in rice quality breeding,in order to accelerate quality breeding process of rice,taking 10 rice cultivars in cold region as experimental materials,amylose content of fine grain,brown rice,milled rice flour and brown rice flour were determinated by near infrared grain analyzer,modified standard method and the single gain method.The results showed that the applicable scope of three determination methods was different with analysis features.The results of singlegain method were reliable which were parallel with that of modified standard method,it could provide an effective determination method for genetic analysis of rice amylose content and early generation selection.On the basis of the determination results for amylose content of brown rice flour,the grading standard was reestablished by singlegain method.
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备注/Memo
收稿日期:2013-09-21基金项目:“十二五”国家科技支撑计划资助项目(2011 BAD35B02-01-01,2012BAD04B01-01-01);佳木斯大学校级人才培养计划资助项目(RC2010-025)第一作者简介:马文东(1980-),男,黑龙江省桦南县人,硕士,助理研究员,从事水稻遗传育种研究。E-mail:sdsmawendong@163.com。通讯作者:李修平(1981-),女,山东省莱西市人,博士,副教授,从事作物遗传育种与生物技术研究。E-mail:lixiuping200@163.com。