MA Gui lan,BAO Yan min,XU Shui lin,et al.Effect Comparison of Fetal Bovine Serum and Newborn Bovine Serum for Animal Cell Culture in vitro[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):65-68.
胎牛血清和新生牛血清体外培养动物细胞的效果比较
- Title:
- Effect Comparison of Fetal Bovine Serum and Newborn Bovine Serum for Animal Cell Culture in vitro
- 文章编号:
- 1002-2767（2014）02-0065-04
- Keywords:
- fetal bovine serum; newborn bovine serum; adherent cell culture; growth curve; colony formation rate
- 分类号:
- S852.5
- 文献标志码:
- A
- 摘要:
- 为了比较进口胎牛血清、国产胎牛血清和国产新生牛血清的细胞培养效果，以2批进口胎牛血清、3批国产胎牛血清和2批国产新生牛血清为试验材料，采用细胞贴壁传代培养、最大增殖浓度和倍增时间以及集落形成率3种方法，比较了7批供试血清对VERO、CHO-K1、MDCK和Hela 4种细胞的培养效果。结果表明：连续传代培养时所有牛血清均有较好的促细胞生长的效果；进口胎牛血清、国产胎牛血清和国产新牛血清对4种细胞的平均最大增殖密度为67.8×104、62.1×104和64.8×104cfu?mL-1，平均倍增时间为22.2、22.5和22.7 h；对VERO、CHO-K1和Hela细胞的平均集落形成率为59.2%、57.6%和50.9%。3种牛血清对群体性细胞培养时效果差别不明显，对单细胞克隆培养时胎牛血清优于新生牛血清。
- Abstract:
- In order to compare the culture effectiveness of imported,domestic and domestic newborn bovine serum for animal cells in vitro ,taking 2 batches of imported fetal bovine serum,3 batches of domestic fetal bovine serum and 2 batches of domestic newborn bovine serum as materials,adherent cell subculture,low cell density growth curve and colony efficiency three methods were used to compare the cells culture effect of four cells of VERO,CHOK1,MDCK and Hela.The results showed that all bovine serum had better cell growth promoting effect by continuous subculture;average maximum density of the imported fetal bovine serum,fetal bovine serum and domestic new domestic bovine serum were 67.8×104,62.1×104 and 64.8×104 cfu?mL-1respectively,average doubling time were 22.2,22.5 and 22.7 h;the average colony formation rate of VERO,CHOK1 and Hela cells were 59.2%,57.6% and 50.9%.The effect of three types of bovine serum on different groups of cells in vitro were not obvious,the individual cells clone culture fetal bovine serum were better than newborn calf serum.
参考文献/References:
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备注/Memo
收稿日期：2013-09-26基金项目：西北民族大学中央高校基本科研业务费专项资金资助项目（31920130066）；甘肃省科技重大专项资助项目（1203-FKDA030）；国家科技基础平台资助项目（2005 DKA21101-29）第一作者简介：马桂兰(1983-)，女，回族，甘肃金昌县人，在读硕士，从事动物血清研发和质量管理工作。E-mail:441407984@qq.com。通讯作者：乔自林（1976-），男，学士，高级实验师。E-mail:670267497@qq.com。