[1]董学志,胡林双,魏琪,等.马铃薯黑胫病菌分离纯化体系的建立[J].黑龙江农业科学,2013,(07):45-48.
　DONG Xue-zhi,HU Lin-shuang,WEI Qi,et al.Establishment of Isolation and Purification System of Potato Black Leg Pathogen[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):45-48.
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马铃薯黑胫病菌分离纯化体系的建立

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年07 页码: 45-48 栏目: 出版日期: 2013-07-30
Title:
Establishment of Isolation and Purification System of Potato Black Leg Pathogen
文章编号:
1002-2767（2013）07-0045-04
作者:
董学志1; 2; 胡林双2; 魏琪2; 邱彩玲1; 2; 闵凡祥2; 杨松3; 李永刚1
1.东北农业大学 农学院，黑龙江 哈尔滨 150030；
2.黑龙江省农业科学院 植物脱毒苗木研究所，黑龙江 哈尔滨 150086；
3.黑龙江省华山监狱，黑龙江 五大连池 164153
Author(s):
DONG Xue-zhi1; 2; HU Lin-shuang2; WEI Qi2; QIU Cai-ling1; 2; MIN Fan-xiang2; YANG Song3; LI Yong-gang1
1.College of Agriculture,Northeast Agricultural University,Harbin,Heilongjiang 150030；
2.Virus-free Seedling Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086；
3.Huashan Prison in Heilongjiang Province,Wudalianchi,Heilongjiang 164153
关键词:
马铃薯黑胫病; 分离; 纯化; 菌株保存; 致病性鉴定
Keywords:
potato black leg; isolation; purification; strains preservation; identification of pathogenic
分类号:
S435.32
文献标志码:
A
摘要:
为了指导马铃薯黑胫病相关试验，利用CVP培养基，对疑似感染了黑胫病的马铃薯植株进行病原菌的分离、鉴定和纯化，并对菌株的3种保存方法进行比较和探讨，同时采用病原菌回接的方法对菌株致病性进行鉴定，最终成功建立了马铃薯黑胫病菌分离纯化体系。
Abstract:
In order to guide relevant test of potato black leg,CVP medium was used to isolate,identify and purify the pathogen of the potato plant that was suspected infected with black leg.Three preservation methods of strain were compared and discussed.Inoculation test were used to identify the virulence of pathogenic.At last,the separation and purification system of potato black leg pathogen was established successfully.

参考文献/References:

[1] 牛志敏.马铃薯黑胫病的发生与防治[J].中国马铃薯,2002,16(2):116-117.
[2]孙秀梅.黑龙江省马铃薯黑胫病的发生与防治[J].中国农村小康科技,2005(12):44.
[3]于恒纯,滕丽雅,闫明宇.黑龙江省马铃薯细菌病害调查初报[J].中国马铃薯,2003,7(2):122-123.
[4]Elphinstona T G.Contamination of progeny tubers of potato plants by seed and leaf borne Erwinia carotorora[J].Potato Research,1986,29(1):77-93.
[5]Schaad N W，Jones J B，Chun W.植物病原细菌鉴定实验指导[M].3版.赵廷昌，译.北京:中国农业科学技术出版社,2011:43-44.

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备注/Memo

收稿日期：2013-03-28
基金项目：黑龙江省青年科学基金资助项目（QC2011C082）;国家马铃薯产业技术体系资助项目(CARS-10-P/4);“十二五”国家科技支撑计划资助项目（2012BAD06B02）
第一作者简介：董学志（1983-），男，黑龙江省哈尔滨市人，在读硕士，研究实习员，从事马铃薯病害研究。E-mail:dongxuezhi_96@126.com。

更新日期/Last Update: 2014-10-30