LIU Chun-lai,LIU Xing-long,WANG Ke-qin,et al.A Fungal Spore Separation Device of Solid State Fermentation For Laboratory[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):36-37.
种实验室用固态发酵真菌孢子分离装置的研制
- Title:
- A Fungal Spore Separation Device of Solid State Fermentation For Laboratory
- 文章编号:
- 1002-2767（2013）03-0036-02
- Keywords:
- biocontrol fungi; conidia; separation device; efficiency
- 分类号:
- S476.1
- 文献标志码:
- A
- 摘要:
- 为获得高纯度孢子粉，针对生防真菌孢子粒径微小，易悬浮于空气中，利用气旋原理，研制出实验室用固态发酵真菌孢子分离装置。该装置由物料粉碎机、沉降分离器、气流循环系统和风机等构成。100 g自然阴干的蜡蚧轮枝菌固体发酵培养物，经孢子分离器处理15 min，分离收获到70%以上的蜡蚧轮枝菌分生孢子，且获得的孢子粉重量占分离前培养物重量的5%。
- Abstract:
- In order to obtain high purity spore powder,according to small size of biocontrol fungi spore and easily suspended in the air,a fungal spore separating device was developed to collect spores from solid state fermentation for the laboratory by the cyclone principle.The device consists of material crusher,separator,an air circulation system and fan etc..More than 70% of Lecanicillium lecanii conidia in 100 gram of the natural shade cultures of solid fermentation were harvested by the device for 15 minutes,and the weight of spore powder was only 5% of original sample.
参考文献/References:
[1] 冯明光，李增智.白僵菌及其应用[C]//陈涛.有害生物的微生物防治原理和技术.武汉：湖北科技出版社，1995:217-244.
[2]Deshpande M V.Mycopesticide production by fermentation:Potential and challenges[J].Critical Reviews in Microbiology,1999,25(3):229-243.
[3]Enabling Technology for Product Development.The Mycoharvest 5b[EB/OL].(2012-07-04)[2012-07-13].http://www.mycoharvester.info/. 该文作者还有杨帆和邵天玉，单位同第一作者
相似文献/References:
[1]刘春来,李新民,王克勤,等.微量元素及维生素对蜡蚧轮枝菌分生孢子的影响[J].黑龙江农业科学,2008,(04):9.[doi:10.11942/j.issn1002-2767.2008.04.0009]
LIU Chun-lai,LI Xin-min,WANG Ke-qin,et al.Effects of Micro Elements and Vitamins onSporulation of Verticillium lecanii[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(03):9.[doi:10.11942/j.issn1002-2767.2008.04.0009]
备注/Memo
收稿日期：2012-12-16
基金项目：国家国际科技合作专项资助项目（2012DFR 30810）
第一作者简介：刘春来（1975-），男，山东省平度市人，硕士，副研究员，从事微生物农药研究。E-mail:liuchunlai@163.com。
通讯作者：李新民(1964-),男，甘肃省成县人，硕士，研究员，从事微生物农药研究。E-mail：xinmin63@yahoo.com.cn。