SONG Wei-feng.Study on Abstracting and Detecting Method of Osthol in Frutus cnidii[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(02):104-105.
蛇床子中蛇床子素的提取及检测方法的研究
- Title:
- Study on Abstracting and Detecting Method of Osthol in Frutus cnidii
- 文章编号:
- 1002-2767（2013）02-0104-02
- Keywords:
- HPLC; Frutus cnidii; osthol
- 分类号:
- R285.5
- 文献标志码:
- A
- 摘要:
- 为探索蛇床子素的提取捡测方法,通过正交试验方法对蛇床子素的提取工艺进行优化研究。结果表明:蛇床子的最佳提取工艺为用5倍量95%乙醇55℃水浴震荡提取3次,每次3h,提取量为8.463mg.g-1。该研究建立了蛇床子素的高效液相色谱法检测方法,色谱条件:流动相,色谱甲醇∶水(60∶40);柱温35℃;紫外检测器检测波长322nm;进样量10μL;流速1.0mL.min-1。该方法能够准确地对蛇床子素进行定性和定量检测。
- Abstract:
- In order to explore the detection method to extract osthol,the optimal extraction process of the Frutus cnidii was determined through orthogonal experiments.The results showed that shocking and extracting it 3 times with 5 times of the amount of 95% ethanol in 55℃ water bath,3 h each time,the extraction amount was 8.463 mg?g-1.The detecting method of osthol was also discussed with HPLC,chromatographic condition:mobile phase,chromatographic pure methanol and water(60∶40);column temperature was 35℃;and extra-purple detector was used,detection wave length was 322 nm;the injection volume was 10 μL,flow rate was 1.0 mL?min-1-.The experiment indicated that the method could be used to detect the osthol exactly.
参考文献/References:
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备注/Memo
收稿日期：2012-11-28