[1]赵贵兴.交联酶聚集体的生物催化[J].黑龙江农业科学,2013,(01):120-124.
　ZHAOGui-xing.Cross-LinkedEnzymeAggregatesbyBiologicalCatalysis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):120-124.
点击复制

交联酶聚集体的生物催化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年01 页码: 120-124 栏目: 出版日期: 2013-01-31
Title:
Cross-LinkedEnzymeAggregatesbyBiologicalCatalysis
文章编号:
1002-2767（2013）01-0120-05
作者:
赵贵兴1; 2
1.哈尔滨商业大学，黑龙江哈尔滨150076；
2.黑龙江省农业科学院大豆研究所，黑龙江哈尔滨150086
Author(s):
ZHAOGui-xing1; 2
1.HarbinUniversityofCommerce，Harbin,Heilongjiang150076；2.SoybeanResearchInstituteofHeilongjiangAcademyofAgriculturalSciences,Harbin,Heilongjiang150086
关键词:
 ; 生物催化;  无载体;  固定化;  交联酶聚集体;  下拉域;  自组系统
Keywords:
biologicalcatalysis; carrier-free; immobilization; cross-linkedenzymeaggregates; dropdomain; self-organizedsystem
分类号:
Q814
文献标志码:
A
摘要:
 对一种崭新的无载体酶固定化技术——聚集体(Cross-linked Enzyme Aggregates,CLEAs)技术进行了文献综述。CLEAs技术是一种将蛋白质先沉淀后交联形成不溶性的、稳定的固定化酶。交联酶聚集体技术现已用于大量的合成反应。在沉淀和交联所需但具有潜在致变性化学品缺失的情况下,融合蛋白会将目的酶的相对选择性聚集体或其自组系统转为不溶性微粒,因而具有强劲的发展动力。在选定的生物转化中,用于多轮间歇式反应不溶蛋白颗粒的回收已经得到论证。然而,对于全连续生物催化过程应用来说,低耐机械应力和高压缩性依然是无载体酶颗粒所需要考虑的问题。
Abstract:
Firstly,anewcarrier-freeimmobilizedenzymetechnology-aggregates(Cross-linkedEnzymeAggregates,CLEAs)technologyhasbeenreviewed.CLEAstechnologyisakindofproteindepositedbeforecross-linkingtoforminsoluble,stabilityoftheimmobilizedenzyme.Cross-linkedenzymeaggregatestechnologyisnowusedinalargenumberofsynthesisreaction.Intheprecipitationandcross-linkingrequiredbuthaspotentialmutagenicityofchemicalsisabsent,fusionproteinwillturnobjectiveenzymerelativelyselectiveaggregatesorevenitsself-organizingsystemsintoinsolubleparticles,whichhasastrongdevelopmentmomentum.Inselectedbiotransformation,formanyroundsofintermittenttypereactioninsolubleproteinparticlesrecyclinghasbeendemonstratedby.However,forthecontinuousbiocatalyticprocessapplications,lowresistancetomechanicalstressandhighcompressibilityarestillthequestionthatcarrier-freeenzymeparticlesneedtoconsider.

参考文献/References:

[1]胥成浩，农向.纤维素酶固定化的最新研究进展[J].吉林农业，2011，249(11):46.
[2]谭碧君.固定化酶制备及应用的研究进展[J].上海畜牧兽医通讯，2011(1):12-15.
[3]AytarBS,BakirU.Preparationofcross-linkedtyrosinaseaggregates[J].ProcessBiochem,2008,43:125-131. [4]BetancorL,LuckariftHR.Bioinspiredenzymeencapsulationforbiocatalysis[J].TrendsBiotechnol,2008,26:566-572.
[5]CabirolFL,TanPL,TayB,etal.Linumusitatissimumhydroxynitrilelyasecrosslinkedenzymeaggregates:arecyclableenantioselectivecatalyst[J].AdvSynthCatal,2008,350:2329-2338.
[6]FujitaY,MieM,KobatakeE.Constructionofnanoscaleproteinparticleusingtemperature-sensitiveelastin-likepeptideandpolyasparticacidchain[J].Biomaterials,2009,30:3450-3457.
[7]HanefeldU,GardossiL,MagnerE.Understandingenzymeimmobilization[J].ChemSocRev,2009,38:453-468.
[8]HickeyAM,NgamsomB,WilesC,etal,Amicroreactorforthestudyofbiotransformationsbyacross-linkedc-lactamaseenzyme[J].BiotechnologyJ.，2009,4:510-516.
[9]KimMI,KimJ,LeeJ,etal.Cross-linkedenzymeaggregatesinhierarchically-orderedmesoporoussilica:asimpleandeffectivemethodforenzymestabilization[J].BiotechnolBioeng,2007,96:210-218.
[10]PoulsenN,BerneC,SpainJ,etal.SilicaimmobilizationofanenzymethroughgeneticengineeringofthediatomThalassiosirapseudonana[J].AngewChem,2007,119:1875-1878.
[11]RajanA,AbrahamTE.Studiesoncrystallizationandcross-linkingoflipaseforbiocatalysis[J].BioprocessBiosystEng，2008,31:87-94.
[12]RajendhranJ,GunasekaranP.ApplicationofcrosslinkedenzymeaggregatesofBacillusbadiuspenicillinGacylasefortheproductionof6-aminopenicillanicacid[J].LettApplMicrobiol,2007，44:43-49.
[13]SchoevaartR,WolbersMW,GolubovicM,etal.Preparation,OptimizationandstrucutresofCross-linkedAggregates(CLEAs)[J].BiotechnolLett,2010,32:341-349.
[14]SchwarzA,ThomsenMS,NidetzkyB.Enzymaticsynthesisofb-glucosylglycerolusingacontinuous-flowmicroreactorcontainingthermostableb-glycosidehydrolaseCelBimmobilizedoncoatedmicrochannelwalls[J].BiotechnolBioeng,2009,103:865-872. [15]ZhaoL,ZhengL,GaoG,etal.ResolutionofN-(2-ethyl-6-methylphenyl)alanineviacross-linkedaggregatesofPseudomonassp.lipase[J].JMolCatalB,2008,54:7-12.

相似文献/References:

[1]马勇. 部分小麦种质中抗秆锈病基因的初步分子检测[J].黑龙江农业科学,2013,(01):7.
　MAYong.MolecularDetectionoftheResistanceGene Sr22toStemRustinWheatGermplasm[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):7.
[2]王玉洋,蒋柳青,董雪梅,等. 不同因素对水稻愈伤组织形成的影响[J].黑龙江农业科学,2013,(01):11.
　WANGYu-yang,JIANGLiu-qing,DONGXue-mei,et al.EffectofDifferentFactorsonRiceCallusFormation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):11.
[3]丁佳红,薛正莲,杨超英. 水杨酸对铜胁迫下水稻幼苗膜脂过氧化作用的影响[J].黑龙江农业科学,2013,(01):14.
　DINGJia-hong,XUEZheng-lian,YANGChao-ying.EffectofSalicylicAcidonMembraneLipidPeroxidationinRiceSeedlingsunderCopperStress[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):14.
[4]王家军,于佰双,张瑞萍,等. 龙引扁豆1号与青贮玉米混种技术研究[J].黑龙江农业科学,2013,(01):19.
　WANGJia-jun,YUBai-shuang,ZHANGRui-ping,et al.StudyonMixingPlantingTechnologyofLablabpurpureus（LongyinLentilNo.1）andSilageMaize[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):19.
[5]吕静,韩莉梅,张所有.超氧化物歧化酶模拟物（SODM）对大豆保护酶系统的影响[J].黑龙江农业科学,2013,(01):23.
　LYUJing,HANLi-mei,ZHANGSuo-you.EffectsofSuperoxideDismutaseMimics(SODM)onSoybeanProtectiveEnzymeSystem[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):23.
[6]王岩成,胡焕春,岳萍. 一定体积的水稻种子间隙吸水量的定量试验[J].黑龙江农业科学,2013,(01):27.
　WANGYan-cheng,HUHuan-chun,YUEPing.QuantitativeTestofRiceSeedsGapWaterAbsorptionofCertainVolume[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):27.
[7]唐克. 不同剂量烟嘧磺隆对不同叶龄玉米幼苗的药害影响[J].黑龙江农业科学,2013,(01):53.
　TANGKe.StudyonEffectofDifferentDosesofNicosulfurononSuiyuNo.7atDifferentLeafAges[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):53.
[8]董爱书,胡新,邵晓梅,等. 大豆田苗后施用噻吩磺隆安全性研究[J].黑龙江农业科学,2013,(01):56.
　DONGAi-shu,HUXin,SHAOXiao-mei,et al.StudyonSoybeanSafetyofApplyingThifensulfuronMethylafterSeedling[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):56.
[9]瞿逸舟,阳检,吴林海. 分散农户农药施用行为与影响因素研究[J].黑龙江农业科学,2013,(01):60.
　QUYi-zhou,YANGJian,WULin-hai.TheScatteredFarmers’BehaviorsofPesticideApplicationandtheInfluencingFactors[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):60.
[10]武琳琳,赵秀梅,王丽达,等.柞蚕卵的保存方式对松毛虫赤眼蜂繁殖的影响[J].黑龙江农业科学,2013,(01):65.
　WULin-lin,ZHAOXiu-mei,WANGLi-da,et al.EffectofDifferentStorageModeofAntheraeapernyiEggs onReproductionoftheTrichogrammadendrolimi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):65.

备注/Memo

收稿日期：2012-11-19
作者简介：赵贵兴（1978-），男，吉林省永吉县人，在读博士，副研究员，从事大豆加工及品质分析研究。E-mail:zhaoguixing@163.com。

更新日期/Last Update: 2013-12-31