[1]张元国,李 芳,包艳存,等.芦笋全雄品种WF-8茎尖组培快繁技术研究[J].黑龙江农业科学,2012,(12):21-23.
ZHANG Yuan-guo,LI Fang,BAO Yan-cun,et al.Study on Tissus Culture Rapid Reproduction Technology of Stem Tip of All Male Asparagus Cultivar WF-8[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):21-23.
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ZHANG Yuan-guo,LI Fang,BAO Yan-cun,et al.Study on Tissus Culture Rapid Reproduction Technology of Stem Tip of All Male Asparagus Cultivar WF-8[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):21-23.
芦笋全雄品种WF-8茎尖组培快繁技术研究
《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S]
卷:
期数:
2012年12
页码:
21-23
栏目:
出版日期:
2012-12-10
- Title:
- Study on Tissus Culture Rapid Reproduction Technology of Stem Tip of All Male Asparagus Cultivar WF-8
- 文章编号:
- 1002-2767(2012)12-0021-03
- 分类号:
- S644.6
- 文献标志码:
- A
- 摘要:
- 为了快速建立芦笋全雄品种制种田,对全雄新品种WF-8的父母本组培快繁和过渡移栽技术进行了系统研究。结果表明:适合全雄品种WF-8母本启动成苗的培养基配比为6-BA1.0 mg·L-1+NAA0.1 mg·L-1,适合父本启动成苗的培养基配比为6-BA1.0 mg·L-1+NAA0.05 mg·L-1;适宜父本增殖的培养基为MS+6-BA1.0 mg·L-1+NAA0.1mg·L-1,适宜母本增殖的培养基为MS+6-BA1.0 mg·L-1+NAA 0.05 mg·L-1+KT 0.1 mg·L-1;蔗糖浓度由30 g·L-1增加到35 g·L-1,琼脂浓度由4 g·L-1增加到4.5 g·L-1,可以防止芦笋试管苗玻璃化;移栽成活率高达95.3%。
- Abstract:
- In order to quickly build asparagus male varieties for farm,the study on culture callus and transitional cultivating of a new all male asparagus hybrid “WF-8” parents was studied in detail.The results showed that:the suitable hormone proportion of female parent initiation culture callus was NAA 0.1 mg·L-1+ 6-BA 1.0 mg·L-1,the suitable hormone proportion of male parent initiation culture callus was NAA0.05 mg·L-1+ 6-BA1.0 mg·L-1;The suitable proliferation culture medium for male parent was MS+6-BA1.0 mg·L-1+NAA 0.1 mg·L-1,the suitable proliferation culture medium for female parent was MS+6-BA 1.0 mg·L-1+NAA 0.05 mg·L-1+KT 0.1 mg·L-1;The sucrose concentration increased from 30 g·L-1 to 35 g·L-1 and the agar concentration increased from 4 g?L-1 to 4.5 g·L-1 could prevent the asparagus tissue culture seedling from vitrification;The survival rate of transplanting was 95.3%.
参考文献/References:
[1]周维燕.芦笋组织培养及育种中的应用[M].北京:高等教育出版社,1989:46-63. [2]周维燕,费水章,陆朝福,等.石刁柏超雄株培养技术的研究[J].北京农业大学学报,1991(1):65-75. [3]范双喜.芦笋全雄株快繁研究初报[J].中国蔬菜,1995(1):34-35. [4]马凤桐,郭春慧,张巧绒,等.芦笋优良雄株无性系繁殖的研究[J].西北农业学报,1993,2(4):39-42. [5]于继庆,马秀兰.芦笋茎尖组织培养技术的研究[J].山东农业科学,1991(4):16-20. [6]范双喜,解淑贞,谷建田.石刁柏离体培养快速繁育的研究[J].北京农学院学报,1995,10(1):49-53.
备注/Memo
基金项目:山东省现代农业产业技术体系蔬菜产业创新团队建设资助项目(2009-2012)第一作者简介:张元国(1966-),男,山东省临朐县人,硕士,研究员,从事芦笋组织培养和蔬菜集约化育苗研究。E-mail:zyg66205@163.com。
更新日期/Last Update:
2014-10-27