[1]刘毅.稻草粉混菌同步糖化发酵产柠檬酸的条件优化[J].黑龙江农业科学,2012,(09):102-105.
LIU Yi.Condition Optimization of Citric Acid Production from Rice Straw Powder by Mixed Culture Simultaneous Saccharification and Fermentation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(09):102-105.
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LIU Yi.Condition Optimization of Citric Acid Production from Rice Straw Powder by Mixed Culture Simultaneous Saccharification and Fermentation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(09):102-105.
稻草粉混菌同步糖化发酵产柠檬酸的条件优化
《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S]
卷:
期数:
2012年09
页码:
102-105
栏目:
出版日期:
2012-09-10
- Title:
- Condition Optimization of Citric Acid Production from Rice Straw Powder by Mixed Culture Simultaneous Saccharification and Fermentation
- 文章编号:
- 1002-2767（2012）09-0102-04
- 分类号:
- Q939.9
- 文献标志码:
- A
- 摘要:
- 将糖化菌群与柠檬酸发酵菌混合接种于同一容器中，以经预处理的稻草粉为主要原料发酵柠檬酸，经单因素试验和正交试验，明确产柠檬酸的优化条件。结果表明：混菌同步糖化发酵产柠檬酸的优化条件为，接入柠檬酸发酵种子时间为3 d，黑曲霉发酵时间为5 d，柠檬酸发酵种子接种量10%(v/v)，初始pH 5.5，此时柠檬酸的产量为85.1 g·L-1。
- Abstract:
- Saccharification fungi group was mixed with citric acid fermentation fungi inoculation in the same container,using pretreatment of straw powder as main raw materials to ferment citric acid by single factors experimental and orthogonal experiment.The result indicated that the mixed optimum conditions of simultaneous saccharification and fermentation of citric acid were:inoculation time of citric acid fermented seeds for 3 days,Aspergillus niger fermentation time for 5 days,inoculation quantity of citric acid fermented seeds for 10%(v/v),the initial pH for 5.5,thus,the citric acid production was 85.1 g·L-1.
参考文献/References:
[1]
朱圣东，吴元欣，喻子牛，等
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[2]朱亨政.柠檬酸发酵[J].食品与发酵工业，1994（6）：69-74. [3]刘洁，李宪臻，高培基.纤维素酶活力测定方法评述[J].工业微生物，1994，24(4)：27-32. [4]Wyk V，Mohulatsi M.Biodegradation of wastepaper by cellulose from Trichoderma viride[J].Bioresource Technology,2003，86(1)：21-23. [5]GB/T8269-1998，柠檬酸[S]. [6]张龙翔.生化实验方法和技术[M].北京：人民教育出版社，1981:6-9.
备注/Memo
基金项目：湖南省科技厅科技计划资助项目（2011FJ3131）作者简介：刘毅（1964-），男，湖南省桃源县人，学士，副教授，从事生物化工技术教学与研究工作。E-mail:liuyi3968@163.com。
更新日期/Last Update:
2014-10-27