LUO Zhi-wen,LIU Juan,ZHAO Yong-xun.Enzymatic Extraction of?Eupolyphaga sinensisPolysaccharide Technology Optimization[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(08):102-104.
酶解法提取地鳖虫多糖工艺的优化
- Title:
- Enzymatic Extraction of?Eupolyphaga sinensisPolysaccharide Technology Optimization
- 文章编号:
- 1002-2767（2012）08-00102-03
- Keywords:
- Eupolyphaga sinensispolysaccharide; enzymatic hydrolysis; extraction; extraction condition optimization
- 分类号:
- X174
- 文献标志码:
- A
- 摘要:
- 采用酶解法对地鳖虫多糖进行提取试验,优化提取工艺。通过单因素和正交试验方法，筛选提取条件,测定多糖含量，摸索地鳖虫多糖提取的最佳酶用量、提取时间和温度。结果表明：地鳖虫多糖提取的最佳酶用量为300 μg·g-1，提取温度50℃，提取时间3 h,多糖含量为14.326%。
- Abstract:
- The Eupolyphaga sinensispolysaccharides extraction test was conducted and extraction process was optimized by enzymatic hydrolysis.The extraction condition was selected and the content of polysaccharide was determined through the single factor and orthogonal test method,and the optimum enzyme dosage,extraction time and temperature ofEupolyphaga sinensispolysaccharide were studied.The results showed that the optimum conditionsof Eupolyphaga sinensispolysaccharide was extraction enzyme dosage of 300 μg·g-1,extraction temperature of 50℃,extraction time 3 h,the content of polysaccharides was 14.326%．
参考文献/References:
[1]汪世宏 . 我国药用地鳖虫的研究 [J]. 中药材 ,1990,13(6):11 - 12. [2]张安宁,桂仲争.地鳖虫的利用价值及其开发前景[J].生物学杂志,2008,25(2):59-61. [3]王丹.药用地鳖虫的研究进展[J].科技经济市场,2010(6):108-109. [4]田军鹏,黄文,雷朝亮.地鳖虫药理作用研究概况[J].时珍国医国药,2006,17(3):418-419. [5]暴昕,林晶.中药地鳖虫药用价值介绍[J].中国城乡企业卫生,2011(4):119-120. [6]尹鲁生.土鳖虫中氨基酸的含量[J].中草药,1987,18(2):42. [7]李兴暖,牛力,赵勇.地鳖虫纤溶活性蛋白组分抑制肿瘤作用研究[J].时珍国医国药,2011,22(5):1149-1151. [8]苏德民,沈烈行,徐瑞军，等.土鳖虫的化学成分及药理作用研究进展[J].时珍国药研究,1997,8(2):153. [9]卢颖,江佩芬.土鳖虫化学成分研究[J].中国中药杂志,1992,17(8):487-488.
相似文献/References:
[1]苏云珊.澄清型黑加仑果汁的研制[J].黑龙江农业科学,2012,(11):128.
SU Yun-shan.Development of Blackcurrant Juice Clarification[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(08):128.
备注/Memo
基金项目：黑龙江省教育厅科学技术资助项目(11531384)