ZHANG Lei,SUN Hui-ying,GAO Rong,et al.Effect of Different Concentration PEG Simulating DroughtStress on the Clematis heracleifolia[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(07):69-72.
不同浓度PEG模拟干旱胁迫对大叶铁线莲的影响
- Title:
- Effect of Different Concentration PEG Simulating DroughtStress on the Clematis heracleifolia
- 文章编号:
- 1002-2767（2012）07-0069-04
- 分类号:
- S682.36
- 文献标志码:
- A
- 摘要:
- 大叶铁线莲具有耐旱植物特征，根系长达60 cm以上。为研究其是否能够在大庆草原沙土干旱生境应用于园林绿化，选择大叶铁线莲一年生实生苗为试材，以30%、50%、70%浓度的PEG溶液人工模拟干旱渗透胁迫，测定其在不同干旱胁迫下，叶面积、叶片含水量、根系活力的变化。结果表明：大叶铁线莲的叶面积和叶片的绝对含水量与PEG的浓度及PEG的处理时间呈负相关：其中以30%PEG处理12 h叶片开始出现皱缩，叶面积减少16.67%；50% PEG处理8 h减少21.69%;70% PEG处理4 h减少34.00%。根系活力与PEG的浓度及PEG的处理时间呈负相关，胁迫28 h，30%、50%、70%PEG处理的根系活力分别下降53.36%、72.25%和81.62%。大叶铁线莲在不同干旱胁迫下具有一定的抗旱能力，30%PEG胁迫20 h以内，70%植株能恢复，PEG对恢复后的植株仍有伤害，表现为形态不正常，但能长出新叶；50%PEG胁迫16 h以内，65%植株能恢复；70%PEG胁迫12 h植株就失去生命活力。复水28 h后，30%、50%PEG根系活力各恢复44%和14%；70%PEG处理的根系活力没有恢复。
- Abstract:
- Clematis heracleifolia has drought-tolerant plants characteristics with the root over 60 cm.For the purpose of studying whether it could be used for landscaping in area of drought sand habitat like Daqing grassland，one-year Clematis heracleifoliawas chosen as test materials and 30%，50%，70% PEG simulation drought osmotic stress were used to determine the leaf area，leaf water content，root vigor changes under different drought stress.The results showed that:the leaf area and leaf water content of Clematis heracleifolia had negative correlation with the concentration of the PEG and PEG processing time:30% PEG processing 12 h，leaves began to appear shrink，leaf area reducing 16.67%;50% PEG processing 8 h reduce 21.69%，and 70% PEG processing 4 h reduced by 34.00%.Root vigor has negative correlation with the concentration of the PEG and PEG processing time.Stressing 28 h，root activity of 30%，50%，70% PEG processing were reduced by 53.36%，72.25%，81.62%.Clematis heracleifolia had certain ability of fighting drought in stress:30% PEG stressing within 20 h，70% plant could resume，but PEG still hurt the restoration of the plant，for the performance of the form of the Clematis heracleifolia was not normal，but Clematis heracleifolia could leaf out;50% PEG stressing within 16 h，65% plant could restore;70% PEG stressing 12 h，plant lost life energy.After watering 28 h，30%，50% PEG root activity each restoration 44% and 14%，70% PEG root vigor had not restored.
参考文献/References:
[1] 岳桦，孙笑丛 .PEG 渗透胁迫对对开蕨生理特性影响 [J]. 北方园艺， 2011(1):91 - 94. [2]吴银明，王平，刘洪升，等.分根PEG胁迫对羊草幼苗植物量、活性氧代谢及脯氨酸含量的影响[J].甘肃农业大学学报，2008，4(2):114-119. [3]华东师范大学生物系植物生理教研组.植物组织含水量测定，植物生理学试验指导[M].北京:高等教育出版社，1980:225. [4]孟健男，于晶，苍晶，等.PEG胁迫对两种冬小麦苗期抗旱生理特性的影响[J].东北农业大学学报，2011，42(1):40-44. [5]Levit t A D.Responses of plant to environmental stresses[M].〖JP〗New York:Academic Press，1990：89-99. [6]Schonefeld M A，Johns on R C，Carver B F，et al.Water relations in winter wheat as drought resistance indicators[J].Crop Science，1988，28(3):526-531. [7]刘祖祺，张石城.植物抗性生理[M].北京:中国农业出版社，1994:222-223. [8]郝再彬，苍晶，徐仲，等.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社，2004：24-25. [9]周宜君，金朝，马文文，等.植物抗逆分子机制研究进展[J].中央民族大学学报:自然科学版，2006，15(2):169-175.
相似文献/References:
[1]牛同旭,郑桂萍,吕艳东,等.寒地水稻种质资源芽期抗旱性筛选与评价[J].黑龙江农业科学,2018,(01):1.[doi:10.11942/j.issn1002-2767.2018.01.0001]
NIU Tong-xu,ZHENG Gui-ping,LYU Yan-dong,et al.Screening and Evaluation of Drought Resistance of Rice Germplasm Resources in Cold Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(07):1.[doi:10.11942/j.issn1002-2767.2018.01.0001]
[2]李柳柳,葛人杰,王亚丽,等.干旱胁迫对黄芩幼苗保护酶活性的影响[J].黑龙江农业科学,2018,(12):14.[doi:10.11942/j.issn1002-2767.2018.12.0014]
LI Liu-liu,GE Ren-jie,WANG Ya-li,et al.Effects of Drought Stress on Protective Enzymes Activitiesof Scutellaria baicalensis Seedlings[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(07):14.[doi:10.11942/j.issn1002-2767.2018.12.0014]
备注/Memo
基金项目：东北林业大学本科生国家级创新实验资助项目（101022517）