ZHOU Di,SUN Lian-fa,CHEN Li-jun.Effect of Different Media on the Induction Rate of Embryoid in Wheat Anther Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):9-12.
不同诱导培养基对小麦花药培养胚状体诱导率的影响
- Title:
-
Effect of Different Media on the Induction Rate of Embryoid in Wheat Anther Culture
- 文章编号:
- 1002-2767（2012）06-0009-04
- Keywords:
- wheat anther culture; synthetic hexaploid wheat; induction media; induction rate of embryoid
- 分类号:
- S512.1
- 文献标志码:
- A
- 摘要:
- 为明确W14-F诱导培养基对黑龙江省春小麦品种及人工合成小麦材料的花药培养胚状体诱导率，以6份人工合成小麦材料和4个普通小麦品种为材料，采用4种诱导培养基W14-F、MS、N6和C17进行花药培养比较试验。结果表明：不同的诱导培养基对于小麦材料的胚状体诱导率不同，4种诱导培养基的胚状体诱导能力依次为W14-F> C17 > N6 > MS，W14-F培养基胚状体诱导率明显高于其它3个培养基。人工合成小麦和普通小麦在4种培养基上都得到了相近的胚状体诱导率，说明W14-F培养基同样适合人工合成小麦。利用该技术体系培养人工合成小麦与普通小麦的杂种F1材料，得到了较高的诱导率，这为该技术体系直接应用于利用人工合成小麦改良小麦品种抗病抗逆性研究中提供了可靠的依据。
- Abstract:
- In order to clarify the effect of W14-F induction media on induction rate of embryoid of synthetic hexaploid wheat and common wheat,6 synthetic hexaploid wheat accessions and 4 common wheat varieties were cultivated on four induction media:W14-F，MS，N6 and C17.The results showed that different induction media got different induction rate of embryoid,W14-F>C17>N6>MS,the induction rate of W14-F was higher than that of the other media evidently;The research on the comparison of synthetic hexaploid wheat and common wheat showed that there was no difference between synthetic hexaploid wheat and common wheat cultivars adapted in Heilongjiang on the rate of embryoid in anther culture.The F1 derived from crosses of synthetic hexaploid wheat and common wheat got a higher induction rate of embryoid by this technology system,indicating that W14-F medium could be important for enhancing efficiency of breeding for resistance to bio- and abio-stress using synthetic hexaploid wheat.
参考文献/References:
[1]张晓丽，魏俊杰.小麦花药培养的影响因素[J].农业科技报，2007(4):138-139. [2]徐龙珠.基因型对小麦花药培养的影响[J].河南师范大学学报:自然科学版,1992(2):100-103. [3]郝云凤,莫日·陶格斯,樊俊峰,等.春小麦杂交组合配制和基因型差异对花培的影响[J].内蒙古农业科技,2003(5):14. [4]海燕,康明辉,郭景战,等.稀土和基因型对小麦花培绿苗分化率的影响[J].华北农报,2006，21(2)：234-236. [5]李守岭,庄南生.植物花药培养及其影响因素研究进展[J].亚热带植物科学,2006,35(3):76-80. [6]王培,陈玉蓉.固体培养基上浸润培养提高花粉植株诱导率的研究[J].华北农学报,1992,7(3):59-65. [7]冬梅.影响小麦花药愈伤组织诱导和植株再生的因素[J].青海农业科技,2000(4):11-12. [8]温之雨,王培.小麦花药愈伤组织诱导和新品种冀42选育的研究[J].农业生物技报,2000,8(2):1-4. [9]陈保锋.不同培养基对小麦花药愈伤组织诱导和分化的影响[J].技术与市场,2008(3):46-47.
相似文献/References:
[1]赵远玲,等.人工合成小麦的颖壳硬度和穗发芽抗性关系研究[J].黑龙江农业科学,2008,(04):22.[doi:10.11942/j.issn1002-2767.2008.04.0022]
ZHAO Yuan-ling,SUN Lian-fa,et al.Study on Relationship between Glume Hardness and SproutingResistance in Synthetic Hexaploidy Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(06):22.[doi:10.11942/j.issn1002-2767.2008.04.0022]
[2]赵远玲,孙连发,高凤梅,等.人工合成小麦衍生春小麦新品种龙春1号[J].黑龙江农业科学,2020,(11):128.[doi:10.11942/j.issn1002-2767.2020.11.0128]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(06):128.[doi:10.11942/j.issn1002-2767.2020.11.0128]
备注/Memo
基金项目： 科技部国际合作资助项目（2009DFA32510）