WU Shu-qi,MA Jin-ping,WANG Jia-jia,et al.Effects of Different Pretreatments on Enhancing ReducingSugar Hydrolysis Result of Cotton Stalk[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(05):74-77.
不同预处理提高棉花秸秆还原糖酶解效果的研究
- Title:
- Effects of Different Pretreatments on Enhancing ReducingSugar Hydrolysis Result of Cotton Stalk
- 文章编号:
- 1002-2767（2012）05-0074-04
- 分类号:
- S811.5
- 文献标志码:
- A
- 摘要:
- 为缓解饲草短缺的问题，采用硫酸处理、微波处理、蒸汽爆破处理等9种方法预处理棉花秸秆，软化棉花秸秆中的木质素，增强饲喂时的适口性，提高棉花秸秆的利用率。结果表明：蒸汽爆破处理棉花秸秆的损失量最大，为39.00%。纤维素含量除了蒸汽爆破处理较低，其它处理均高于对照。木质素含量，除氨化（16℃）与对照接近外，其它的处理均低于对照。对预处理的棉花秸秆分别添加筛选自牛粪一组纤维素降解复合系和绿色木霉进行酶解试验。酶解结果表明：微波处理、氨化处理（16℃）、碱+微波处理、双氧水处理、蒸汽爆破处理绿色木霉的活性强于纤维素复合酶，碱/微波处理、氨化处理（30℃）、碱处理、硫酸处理的预处理棉花秸秆进行酶解效果纤维素复合酶的作用优于绿色木霉的效果。微波处理、氨化处理、碱+微波处理、双氧水处理、硫酸预处理酶解的效果较好。碱+微波波处理失重率最高，达到19.32%、酶解率最高，达到32.20%；硫酸处理糖化率最高，达到18.20%、转化率最高，达到20.23%；碱+微波处理葡萄糖得率最高，达到1.013%。
- Abstract:
- With the aim of alleviating the shortage of forage problems,using nine pretreatments of cotton stalk,such as sulfuric acid,microwave processing,steam explosion processing and so on,to soften the lignin in cotton stalks,enhance the palatability of feeding and improve the efficiency of cotton stalk.The results showed that:under steam explosion,the loss rate of cotton straw was the biggest,39.00%.cellulose content of the pretreatments were higher than blank control,except the steam explosion process.Lignin content of pretreatments were lower than the control,except for ammonification (16℃) treatment nearly with the control.The enzyme solution experiment was conducted by adding cellulose degradation of composite performance screening from a group of cow dung and trichoderma viride on the pretreatment cotton stalk respectively.Crude enzyme liquid was extracted.The pretreatment cotton straw,which was pretreated with microwaves,ammonia water,alkali and microwaves,hydrogen peroxide and steam exploded,was decomposed by cellulase of Trichoderma vride more than cellulose degrading microbial flora.And others cellulose degrading microbial flora more than Trichoderma vride.Overall microwaves,ammonia water,alkali and microwaves,hydrogen peroxide,sulphuric acid pretreatment enzymolysis were better than others.Alkali and microwaves lose weight was the highest,19.32%,and the rate of enzymolysis was the highest,32.20%.Sulphuric acid pretreatment glycosylated ratio was the most of 18.20%and the highest convert ratio was 20.23%.Alkali and microwaves glucose yield was the highest,1.013%.
参考文献/References:
[1]杨涛.水稻秸秆纤维素发酵转化燃料乙醇的研究[D].长沙：湖南农业大学,2008. [2]宋安东,任天宝,谢慧,等.化学与处理对玉米秸秆酶解糖化效果的实验[J].化学与生物工程，2006,23(8):22-27. [3]Haykir. A comparative study on lignocellulose pretreatments for bioethanol production from cotton stalk[J].New biotechnology,2009,25(1):253-254. [4]邓辉,李春,李飞,等.棉花秸秆糖化碱预处理条件优化[J].农业工程学报,2009,25(1):208-212. [5]张琴,李艳宾,岳耀峰,等.棉花秸秆的微生物降解及糖化工艺研究[J].食品与发酵工业,2009,35(2):142-145. [6]李为,薛红蕾,王江丽,等.一组高效棉花秸秆降解菌复合系的构建及其降解特性的研究[J].新疆农业科学，2009,46(1):72-77. [7]尹礎,夏乐先,柳建设,等.一株纤维素降解菌株的分离鉴定及产酶特征研究[J].环境科学与技术,2009,32(3):50-53. [8]刘德海,杨玉华,张发旺,等.饲用纤维素酶活力的测定方法的探讨[J].饲料工业,2002,23(4):34-35. [9]魏敏,雒秋江,王东宝,等.棉花秸秆作为绵羊粗饲料的研究[J].草食家畜,2003（3）:47-49. [10]Zhu Shengdong,Wu Yuanxin,Yu Ziniu,et al.Pretreatment by microwave/alika of rice straw and its enzymic hydrolysis[J].Prosess Chemistry,2005,40,3082-3086. [11]Riedel K,Bronnenmeier K.Active-site mutations which change the substratespecificity of the Clostridium stercorarium cellulase Ce1Z implications for synergism[J].Eur J Biochem,1999,262(1):218-223.
相似文献/References:
[1]张雪峰,胡 滨,金 丹.不同外源药剂预处理对低温胁迫下玉米种子萌发的影响[J].黑龙江农业科学,2011,(04):69.
ZHANG Xue-feng,HU Bin,JIN Dan.Effect of Exogenous Chemical Substances Pretreatment onMaize Seed Germination under Low Temperature Stress[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(05):69.
[2]刘 新,李 静,晏飞来,等.木质纤维素预处理工艺的研究现状[J].黑龙江农业科学,2010,(02):112.
LIU Xin,LI Jing,YAN Fei-lai,et al.Research Progress in Pretreatment of Lignocellulose[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(05):112.
[3]荀冬雪,米 平,张身厚,等.超声波预处理对褐菇多糖提取工艺的研究[J].黑龙江农业科学,2010,(12):128.
XUN Dong-xue,MI Ping,ZHANG Shen-hou,et al.Study on Extraction Technology of Polysaccharide fromPortabella by the Ultrasonic Pretreatment[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(05):128.
[4]杜娟,廖新福,热比古丽?哈力克,等.桑葚热风烘干工艺研究[J].黑龙江农业科学,2017,(07):66.[doi:10.11942/j.issn1002-2767.2017.07.0066]
DU Juan,LIAO Xin-fu,RebiguliHalike,et al.Study on Drying Technology of Mulberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(05):66.[doi:10.11942/j.issn1002-2767.2017.07.0066]
[5]程晓东,索玉祥,赵胜楠,等.厌氧消化预处理抗生素废物技术研究进展[J].黑龙江农业科学,2017,(11):113.[doi:10.11942/j.issn1002-2767.2017.11.0113]
CHENG Xiao-dong,SUO Yu-xiang,ZHAO Sheng-nan,et al.Research Progress of Anaerobic Digestion Pretreatment of Antibiotic Waste[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(05):113.[doi:10.11942/j.issn1002-2767.2017.11.0113]
[6]杜娟,廖新福,热比古丽?哈力克,等.热风烘干对桑葚制干效果的影响[J].黑龙江农业科学,2018,(03):113.[doi:10.11942/j.issn1002-2767.2018.03.0113]
DU Juan,LIAO Xin-fu,REBIGULIHalike,et al.Effects of Hot Air Drying on the Drying Effect of Mulberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(05):113.[doi:10.11942/j.issn1002-2767.2018.03.0113]
备注/Memo
基金项目：国家自然基金资助项目(3096026）；“十二五”国家科技支撑计划资助项目（2011BAD17B05）；国家牧草产业技术体系塔里木综合试验站资助项目(CARS-35)