[1]宿飞飞,马 纪,胡林双,等.植物源杀菌剂对马铃薯组织培养中青霉菌的抑菌效果[J].黑龙江农业科学,2012,(03):81-83.
　XV Fei-fei,MA Ji,HU Lin-shuang,et al.Impact of Herbal Fungicide on Inhibiting Penicillium in Medium of Potato Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):81-83.
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植物源杀菌剂对马铃薯组织培养中青霉菌的抑菌效果

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2012年03 页码: 81-83 栏目: 出版日期: 2012-03-01
Title:
Impact of Herbal Fungicide on Inhibiting Penicillium in Medium of Potato Tissue Culture
文章编号:
1002-2767（2012）03-0081-03
作者:
宿飞飞1; 马 纪1; 胡林双1; 王绍鹏1; 刘尚武1; 李 勇1; 刘伟婷2; 吕典秋1
1.黑龙江省农业科学院 植物脱毒苗木研究所，黑龙江 哈尔滨 150086；
2.黑龙江省农业科学院，黑龙江 哈尔滨 150086
Author(s):
XV Fei-fei1; MA Ji1; HU Lin-shuang1; WANG Shao-peng1; LIU Shang-wu1; LI Yong1; LIU Wei-ting2; LV Dian-qiu1
1.Virus-free Seedlings Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086;
2.Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086
关键词:
马铃薯; 青霉菌; 污染; 植物源抑菌剂
Keywords:
potato; penicillium; contamination; herbal fungicide
分类号:
S532
文献标志码:
A
摘要:
在马铃薯脱毒试管苗工厂化快繁过程中，为了抑制试管苗中的真菌污染，减少损失，采用平板生长抑制法用3种植物源杀菌剂苦参、中草药和黑农一号，对植物组织培养中青霉菌抑菌效果进行研究。结果表明：在培养基中加入黑农一号抑菌剂抑制青霉菌的效果最好，浓度在2.0%时，抑菌率达100.0%。
Abstract:
In order to inhibit fungal contamination of virus-free potato in vitro plants in rapid propagation and reduce the loss，plating medium inhibition was applied to study the impact on inhibiting penicillium with three kinds of herbal fungicides，including Sophora flavescens，Chinese medicinal herb and Heinong No.1 during tissue culture to inhibit the contamination in test tubes and realize rapid propagation.The results showed that penicillium was inhibited best in medium added with Heinong No.1.The inhibition rate reach 100.0%.

参考文献/References:

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[2]程逸宇，郑建秋，迟卉，等.植物组织培养中真菌污染防治方法研究[J].贵州科学，2006，24(3):40-43.

[3]方中达.植病研究方法[M].3版.北京:中国农业出版社，1998.

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[5]唐启义，冯明光.实用统计分析及其DPS数据处理系统[M].北京：科学出版社，2002.

[6]薛婷，陈丽，李喜宏，等.7种杀真菌剂对蒜薹灰霉菌抑制效果研究[J].河南农业科学，2006（3）：92-96.


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备注/Memo

基金项目：黑龙江省科技厅资助项目（GA08B102、GB07B105）；马铃薯产业创新体系资助项目（NYCYTX-15）；2011年黑龙江省农业科学院科技创新工程重点资助项目

第一作者简介：宿飞飞（1977-），女，黑龙江省肇东市人，在读博士，助理研究员，从事马铃薯组织培养及试管苗脱毒快繁技术的研究及应用.E-mail:xufeifei03@yahoo.com.cn。

更新日期/Last Update: 2014-10-26